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http://dx.doi.org/10.5762/KAIS.2010.11.12.4826

Studies on Bacterial and Fungal Contamination in the Herbal Medicines  

Lee, Jin-Sung (Department of Genetic Engineering, SungKyunKwan University)
Yoon, Young-Sik (Professional Graduate School of Oriental Medicine Wonkwang University)
Publication Information
Journal of the Korea Academia-Industrial cooperation Society / v.11, no.12, 2010 , pp. 4826-4832 More about this Journal
Abstract
The study has been done for about two months through June 2 to July 30 of 2010. The study subjects are three herbal-pharmaceutical companies located in Seoul. Each of them purchased thirteen types of medicinal herbs, then the study did analysis for microbial contamination status of bacteria and fungi. Here, the study focuses on settling out fundamental data bases regarding the investigation standards of microbial contamination. As comparing the study results with contamination limits of bacteria and fungi which are represented by $10^7$ CFU/g and $10^4$ CFU/g in number respectively, the total percentage of fungi contamination which is 12.8% is higher than that of bacteria is only 7.7%. In the DNA homology analysis regarding 16S rRNA gene, 117 of colonization have been selected as study subjects. Including B. cereus composing of resistant spores, soil microbes account for approximately 96.6%. It indicates that it is important to establish collection and preservation systems in handling medicinal herbs. Also, it is critical to manage microbial contamination limits. In conclusion, the study proposes the needs to study on possible mingling of bacteria and fungi in manufacturing process, and microbial contamination status in medicinal herbs.
Keywords
Herbal Medicine; Contamination; Aerobic bacteria; fungus; 16S rRNA;
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