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http://dx.doi.org/10.1186/s40824-016-0078-y

Polypyrrole-incorporated conductive hyaluronic acid hydrogels  

Yang, Jongcheol (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST))
Choe, Goeun (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST))
Yang, Sumi (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST))
Jo, Hyerim (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST))
Lee, Jae Young (School of Materials Science and Engineering, Gwangju Institute of Science and Engineering (GIST))
Publication Information
Biomaterials Research / v.20, no.4, 2016 , pp. 236-242 More about this Journal
Abstract
Background: Hydrogels that possess hydrophilic and soft characteristics have been widely used in various biomedical applications, such as tissue engineering scaffolds and drug delivery. Conventional hydrogels are not electrically conductive and thus their electrical communication with biological systems is limited. Method: To create electrically conductive hydrogels, we fabricated composite hydrogels of hyaluronic acid and polypyrrole. In particular, we synthesized and used pyrrole-hyaluronic acid-conjugates and further chemically polymerized polypyrrole with the conjugates for the production of conductive hydrogels that can display suitable mechanical and structural properties. Results: Various characterization methods, using a rheometer, a scanning electron microscope, and an electrochemical analyzer, revealed that the PPy/HA hydrogels were soft and conductive with ~ 3 kPa Young's modulus and ~ 7.3 mS/cm conductivity. Our preliminary in vitro culture studies showed that fibroblasts were well attached and grew on the conductive hydrogels. Conclusion: These new conductive hydrogels will be greatly beneficial in fields of biomaterials in which electrical properties are important such as tissue engineering scaffolds and prosthetic devices.
Keywords
Polypyrrole; Hyaluronic Acid; Hydrogel; Conductive; Biomaterials;
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1 Pires F, Ferreira Q, Rodrigues CA, Morgado J, Ferreira FC. Neural stem cell differentiation by electrical stimulation using a cross-linked PEDOT substrate: expanding the use of biocompatible conjugated conductive polymers for neural tissue engineering. Biochim Biophys Acta BBA-Gen Subj. 2015;1850: 1158-68.   DOI
2 Green RA, Baek S, Poole-Warren LA, Martens PJ. Conducting polymerhydrogels for medical electrode applications. Sci Technol Adv Mater. 2016.
3 Guimard NK, Gomez N, Schmidt CE. Conducting polymers in biomedical engineering. Prog Polym Sci. 2007;32:876-921.   DOI
4 Bendrea A-D, Cianga L, Cianga I. Review paper: progress in the field of conducting polymers for tissue engineering applications. J Biomater Appl. 2011;26:3-84.   DOI
5 Guiseppi-Elie A. Electroconductive hydrogels: Synthesis, characterization and biomedical applications. Biomaterials. 2010;31:2701-16.   DOI
6 Qu B, Chen C, Qian L, Xiao H, He B. Facile preparation of conductive composite hydrogels based on sodium alginate and graphite. Mater Lett. 2014;137:106-9.   DOI
7 Peng R, Yu Y, Chen S, Yang Y, Tang Y. Conductive nanocomposite hydrogels with self-healing property. RSC Adv. 2014;4:35149-55.   DOI
8 Guarino V, Alvarez-Perez MA, Borriello A, Napolitano T, Ambrosio L. Conductive PANi/PEGDA macroporous hydrogels for nerve regeneration. Adv Healthc Mater. 2013;2:218-27.   DOI
9 Kim D, Abidian M, Martin DC. Conducting polymers grown in hydrogel scaffolds coated on neural prosthetic devices. J Biomed Mater Res A. 2004; 71:577-85.
10 Thrivikraman G, Madras G, Basu B. Intermittent electrical stimuli for guidance of human mesenchymal stem cell lineage commitment towards neural-like cells on electroconductive substrates. Biomaterials. 2014;35:6219-35.   DOI
11 Zhu H, Mitsuhashi N, Klein A, Barsky LW, Weinberg K, Barr ML, et al. The role of the hyaluronan receptor CD44 in mesenchymal stem cell migration in the extracellular matrix. Stem Cells. 2006;24:928-35.   DOI
12 Prestwich GD. Hyaluronic acid-based clinical biomaterials derived for cell and molecule delivery in regenerative medicine. J Controlled Release. 2011; 155:193-9.   DOI
13 Segura T, Anderson BC, Chung PH, Webber RE, Shull KR, Shea LD. Crosslinked hyaluronic acid hydrogels: a strategy to functionalize and pattern. Biomaterials. 2005;26:359-71.   DOI
14 Kogan G, Soltes L, Stern R, Gemeiner P. Hyaluronic acid: a natural biopolymer with a broad range of biomedical and industrial applications. Biotechnol Lett. 2007;29:17-25.
15 Yoo HS, Lee EA, Yoon JJ, Park TG. Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering. Biomaterials. 2005;26:1925-33.   DOI
16 Solis MA, Chen Y-H, Wong TY, Bittencourt VZ, Lin Y-C, Huang LL. Hyaluronan regulates cell behavior: a potential niche matrix for stem cells. Biochem Res Int. 2012;2012.
17 Miyake K, Underhill CB, Lesley J, Kincade PW. Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition. J Exp Med. 1990;172:69-75.   DOI
18 Lei Y, Gojgini S, Lam J, Segura T. The spreading, migration and proliferation of mouse mesenchymal stem cells cultured inside hyaluronic acid hydrogels. Biomaterials. 2011;32:39-47.   DOI
19 Ateh D, Navsaria H, Vadgama P. Polypyrrole-based conducting polymers and interactions with biological tissues. J R Soc Interface. 2006;3:741-52.   DOI
20 Collins MN, Birkinshaw C. Hyaluronic acid based scaffolds for tissue engineering-A review. Carbohydr Polym. 2013;92:1262-79.   DOI
21 Abu-Rabeah K, Polyak B, Ionescu RE, Cosnier S, Marks RS. Synthesis and characterization of a pyrrole-alginate conjugate and its application in a biosensor construction. Biomacromolecules. 2005;6:3313-8.   DOI
22 Stewart E, Kobayashi NR, Higgins MJ, Quigley AF, Jamali S, Moulton SE, et al. Electrical stimulation using conductive polymer polypyrrole promotes differentiation of human neural stem cells: a biocompatible platform for translational neural tissue engineering. Tissue Eng Part C Methods. 2014;21: 385-93.
23 Ahuja T, Mir IA, Kumar D. Biomolecular immobilization on conducting polymers for biosensing applications. Biomaterials. 2007;28:791-805.   DOI
24 Shi Z, Gao H, Feng J, Ding B, Cao X, Kuga S, et al. In situ synthesis of robust conductive cellulose/polypyrrole composite aerogels and their potential application in nerve regeneration. Angew Chem Int Ed. 2014;53:5380-4.   DOI
25 Hur J, Im K, Kim SW, Kim J, Chung D-Y, Kim T-H, et al. Polypyrrole/agarosebased electronically conductive and reversibly restorable hydrogel. ACS Nano. 2014;8:10066-76.   DOI
26 Balint R, Cassidy NJ, Cartmell SH. Conductive polymers: Towards a smart biomaterial for tissue engineering. Acta Biomater. 2014;10:2341-53.   DOI
27 O'brien FJ. Biomaterials & scaffolds for tissue engineering. Mater Today. 2011;14:88-95.   DOI
28 Hardy JG, Lee JY, Schmidt CE. Biomimetic conducting polymer-based tissue scaffolds. Curr Opin Biotechnol. 2013;24:847-54.   DOI