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http://dx.doi.org/10.1186/2055-7124-18-8

Effect of osteoconductive hyaluronate hydrogels on calvarial bone regeneration  

Yeom, Junseok (Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH))
Hwang, Byung Woo (Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH))
Yang, Dong Jun (MegaGen Research Institute of Science and Technology)
Shin, Hong-In (Department of Oral Pathology, School of Dentistry, IHBR, Kyungpook National University)
Hahn, Sei Kwang (Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH))
Publication Information
Biomaterials Research / v.18, no.3, 2014 , pp. 67-72 More about this Journal
Abstract
Background: Without exploitation of possibly immunogenic and carcinogenic bone morphogenetic protein, we developed simple but clinically feasible artificial bone graft using osteoconductive hyaluronate (HA) hydrogels and bioactive MegaGen synthetic bone (MGSB). Methods: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. The bone regeneration by the MGSB/HA-GEL hydrogel complex was assessed in the skull of New Zealand white male rabbits in 4 and 8 weeks. Results: HA hydrogels were synthesized by the crosslinking reaction between carboxyl groups of HA and amine groups of gelatin (GEL). Then, artificial bone grafts were prepared by mixing MGSB with HA-GEL hydrogels. In vitro proliferation of preosteogenic cells was enhanced with increasing molecular weight of HA. In addition, histological analysis of dissected tissues with hematoxylin and eosin staining confirmed the effective in vivo bone regeneration by the MGSB/HA-GEL hydrogel complex. The MGSB/HA-GEL hydrogels were well resorbed and partially substituted to the lamellar bone after implantation for 8 weeks. Conclusions: The novel artificial bone graft of MGSB/HA-GEL hydrogel complex for effective bone regeneration might be clinically feasible for further development.
Keywords
Hyaluronic acid; Gelatin; Hydrogel; Synthetic bone; Bone regeneration;
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