Browse > Article

Biological Evaluation of Cellulose Hydrogel with Temperature-Responsive Particles  

Kim, Ah Ram (Department of Chemical Engineering and Biomaterials Seoul National University of Science and Technology)
Park, Hyo Seung (Department of Chemical Engineering and Biomaterials Seoul National University of Science and Technology)
Kim, Sae Soon (Department of Chemical Engineering and Biomaterials Seoul National University of Science and Technology)
Noh, Insup (Department of Chemical Engineering and Convergence Institute of Biomedical Engineering and Biomaterials Seoul National University of Science and Technology)
Publication Information
Biomaterials Research / v.17, no.4, 2013 , pp. 181-186 More about this Journal
Abstract
Carboxymethyl cellulose (CMC) hydrogel has been synthesized in this study by chemically cross-linking of its hydroxyl groups with 1,4-butanediol diglycidyl ether (BDDE), and then converted into a CMC hydrogel by incorporating temperature-responsive (TR) particles with freeze-drying methods. The CMC hydrogel with TR particles was evaluated with physicochemical and biological methods such as color changes over time and temperatures and by in vitro culture of fibroblast cells under the surface of the CMC hydrogels with TR particles, respectively. The CMC hydrogel with TR particles were changed in its colors over temperatures, where its colors depended on the concentrations of TR particles such as 0.1, 0.2 and 0.3%. The results of in vitro assays of the CMC hydrogel with TR particles with bromodeoxy uridune, 3-(4,5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, and neutral red showed excellent cell compatibility compared to those of latex, which was employed as a negative control. Some cells seeded in vitro on the CMC hydrogel were however dead at day 7, even though no significant damages were observed by the extracts of the CMC hydrogel with TR particles. These results indicated that the CMC hydrogel with TR particles should be employed within limited times for its applications in the human contacting areas.
Keywords
Hydrogel; Temperature-Responsive Particles; Carboxymethyl Cellulose;
Citations & Related Records
연도 인용수 순위
  • Reference
1 P. A. Ramires, and E. Milella, "Biocompatibility of poly(vinyl alchol)-hyaluronic acid and poly(vinyl alchol)-gellan membranes crosslinked by glutaraldehyde vapors" J. Materials Science: Materials In Medicine, 13, 119-123 (2002).   DOI   ScienceOn
2 H. Dai, Q. Chen, H. Qin, Y. Guan, D. Shen, Y. Hua, Y. Tang, and J. Xu, "A Temperature-Responsive Copolymer Hydrogel in Controlled Drug Delivery," J. Macromolecules, 39, 6584-6589 (2006).   DOI   ScienceOn
3 T. G. Park, "Temperature modulated protein release from ph/temperature-sensitive hydrogels," J. Biomaterials, 20, 517-521 (1999).   DOI   ScienceOn
4 Y. Luo, K. R. Kirker, and G. D. Prestwich, "Cross-linked hyaluronic acid hydrogel films: new biomaterials for drug delivery," J. Controlled Release, 69, 169-184 (2000).   DOI   ScienceOn
5 A. M. Adel, H. Abou-Youssef, A. A. El-Gendy and A. M. Nada, "Carboxymethylated cellulose hydrogel; sorption behavior and characterization," J. Nat and Sci, 8, 8 (2010).
6 C. Chang, L. Zhang, J. Zhou, L. Zhang, J. F. Kennedy "Structure and properties of hydrogels prepared from cellulose in NaOH/urea aqueous solutions," J. Carbohydrate Polymers, 82, 122-127 (2010).   DOI   ScienceOn
7 X. Feng, R. Pelton, "Carboxymethyl cellulose: poly(vinyl amine) complex hydrogel swelling," J. macromolecules, 40, 1624-1630 (2007).   DOI   ScienceOn
8 H. W. Sung, D. M. Huang, W. H. Chang, and R. N. Huang, J. C. Hsu, "Evaluation of gelatin hydrogel crosslinked with various crosslinking agents as bioadhesives: In vitro study," J. Biomedical Materials Research, 46, 520-530 (1999).   DOI   ScienceOn
9 J. Yeom, S. H. Bhang, B. S. Kim, M. S. Seo, E. J. Hwang, I. H. Cho, J. K. Park, and S. K. Hahn, "Effect of Cross-Linking Reagents for Hyaluronic Acid Hydrogel Dermal Fillers on Tissue Augmentation and Regeneration," J. Bioconjugate Chem, 21, 240-247 (2010).   DOI   ScienceOn
10 M. Rafat, F. Li, P. Fagerholm, N. S. Lagali, M. A. Watsky, R. Munger, T. Matsuura, and M. Griffith, "PEG-stabilized cabodiimide crosslinked collagen-chitosan hydrogels for corneal tissue engineering" J. Biomaterias, 29, 3960-3972 (2008).   DOI   ScienceOn
11 K. P. Vercruysse, D. M. Marecak, J. F. Marecek, and G. D. Prestwich, "Synthesis and in vitro Degradation of New Polyvalent Hydrazide Cross-Linked Hydrogels of Hyaluronic Acid" J. Bioconjugate Chem, 8, 686-694 (1997).   DOI   ScienceOn
12 Jindrich Kopecek, "Hydrogel biomaterials: A smart future?," J. Biomaterials, 28, 5185-5192 (2007).   DOI   ScienceOn
13 A. C. Jen, M. C. Wake, and A. G. Mikos, "Review: Hydrogels for Cell immobilization," J. Biotechnology and Bioengineering, 50, 357-364 (1996).   DOI   ScienceOn
14 K. Kabiri, H. Omidian, M. J. Zohuriaan-Mehr, and S. Doroudiani, "Superabsorbent Hydrogel Composites and Nanocomposites: A Review," J. Polymer Composites, 32, 277-289 (2011).   DOI   ScienceOn
15 김소연, "생체공학적 응용을 위한 고분자 하이드로젤의 연구 동향, " J. Tissue Engineering and Regenerative Medicine, 5, 14-25 (2008).
16 J. L. Drury, D. J. Mooney, "Hydrogels for tissue engineering: scaffold design variables and applications," J. Biomaterials, 24, 4337-4351 (2003).   DOI   ScienceOn
17 S. V. Vlierberghe, P. Dubruel, and E. Schacht, "Biopolymer-based hydrogels as scaffolds for tissue engineering applications: a review," J. Biomacromolecules, 12, 1387-1408 (2011).   DOI   ScienceOn
18 E. R. Gariepy, and J. C. Leroux, "In situ-forming hydrogels-review of temperature-sensitive systems," J. Pharmaceutics and Biopharmaceutics, 58, 409-426 (2004).   DOI   ScienceOn
19 R. M. K Ramanan, P. Chellamuthu, L. Tang, and K. T. Nguyen, "Development of a Temperature-Sensitive Composite Hydrogel for Drug Delivery Applications," J. Biotechnol. Prog, 22, 118-125 (2006).   DOI   ScienceOn
20 Y. Qiu, and K. Park, "Environment-sensitive hydrogels for drug delivery," J. Advanced Drug Delivery Review, 64, 49-60 (2012).   DOI   ScienceOn
21 M. K. Lim, Z. Zhang, W. T. Goh, "An iterative agent bidding mechanism for responsive manufacturing," J. Engineering Applications of Artificial intelligence, 22, 1068-1079 (2009).   DOI   ScienceOn