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An Effect of Resveratrol on the Formation of Lipid Membrane Fragmentations Caused by the Interaction Between Antimicrobial Peptide Pexiganan (MSI-78) and Lipid Bilayer Membranes  

You, Changsoo (Department of Fine Chemistry, Seoul National University of Science & Technology)
Jung, Junho (Department of Fine Chemistry, Seoul National University of Science & Technology)
Lee, Dongkuk (Department of Fine Chemistry, Seoul National University of Science & Technology)
Publication Information
Biomaterials Research / v.17, no.4, 2013 , pp. 168-175 More about this Journal
Abstract
This study has investigated the effect of Resveratrol(3,5,4'-trihydroxy-trans-stilbene) on membrane fragmentation of model lipid bilayer membranes caused by the antimicrobial peptide, Pexiganan (MSI-78). Large unilamellar vesicles (LUVs) of 7:3 POPC/POPG has been used for the model cellular membrane in the study of interaction between biomolecules and lipid molecules. Recently, this model was used to investigate lipid composition-dependent membrane fragmentation and pore-forming mechanism of membrane disruption by Pexigan (MSI-78). Results showed that the peptide induces micelle-like fragments evidenced by the $^{31}P$ NMR signal at 0 ppm compared to the typical powder pattern of unperturbed lipid bilayers. In the present study, Resveratrol molecules were added to the LUVs before the peptide was applied to them. $^{31}P$ spectra were collected for each sample and monitored any changes in the $^{31}P$ NMR spectra. Our observations show that resveratrol inhibits MSI-78 interaction to POPC/POPG lipid bilayer and protect the lipid membrane evidenced by the disappearance of the isotropic $^{31}P$ NMR intensity near 0.0 ppm. We suggest that resveratrol binds to the surface of the POPC/POPG bilayers and alters the properties of the lipid membrane.
Keywords
MSI-78; Pexiganan; Resveratro;
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