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Physical and Biological Evaluations of Cross-linked Hyaluronic Acid Film  

Park, Hyo Seung (Department of Chemical and Biomacromolecular Engineering, Seoul National University of Science and Technology)
kim, Ah Ram (Department of Chemical and Biomacromolecular Engineering, Seoul National University of Science and Technology)
Noh, Insup (Department of Chemical and Biomacromolecular Engineering, Convergence Institute of Biomedical Engineering and Biomaterials, Seoul National University of Science and Technology)
Publication Information
Biomaterials Research / v.17, no.4, 2013 , pp. 153-159 More about this Journal
Abstract
Hyaluronic acid (HA) has been employed as a typical biomaterial in various medical areas such as dermal filler, scaffolds for tissue engineering and drug delivery, and etc. In this report, the HA films were fabricated by controlling the concentrations of 1,4-butanediol diglycidyl ether (BDDE) as a cross-linking agent, and their physical and biological properties have been evaluated. After swelling in water and dehydration by lyophilization of the cross-linked HA films, their morphologies and swelling behaviors after swelling in water for 24 hrs have been observed with scanning electron microscopy and measured with a microbalance, respectively. After homogenizing the films into microparticles, cell viability of the films was indirectly performed on the particles by covering the films with the HA films. Their cell compatibilities were evaluated by the assays of live & dead, thiazolyl blue tetrazolium bromide (MTT), bromodeoxyuridine (BrdU) and neutral red, by using extracts of the microparticles of HA films. The HA particles showed excellent cell compatibilities, showing possibilities of its applications as biomaterials.
Keywords
Biomaterials; Hyaluronic Acid; Film; Biocompatibility;
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