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Effects of Dental Mineral Trioxide Aggregate on Cellular Reactions Involved in Inflammation and Differentiation  

Kim, Jong-Gil (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
Son, Kyung Mi (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
Park, Hee Chul (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
Zhu, Tingting (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
Kwon, Ji Hyun (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
Yang, Hyeong-Cheol (Department of Dental Biomaterials Science and Dental Research Institute, School of Dentistry, Seoul National University)
Publication Information
Biomaterials Research / v.17, no.1, 2013 , pp. 7-12 More about this Journal
Abstract
Mineral trioxide aggregate (MTA) is receiving more attentions in endodontic dental clinics due to its high sealing ability and biocompatibility. Although previous studies have reported the effects of MTA on dental pulp tissue, the mechanisms underlying on inflammation and differentiation at cellular levels are not yet fully understood. In this study, we investigated various molecular aspects of cellular reactions involved in inflammation and differentiation to commercially available MTAs including ProRoot MTA, Ortho MTA and MTA-Angelus. mRNA of COX-2 gene which is involved in inflammatory reaction was not increased by all tested MTAs, and expression of COX-2 by lipopolysaccharide was not severely altered by MTAs, demonstrating that COX-2 is not involved in inflammatory reactions to MTA. The extracts of all MTAs obtained for early 24 hrs increased ALP activities of human dental pulp (HDP) cells, while the extracts of ProRoot MTA and MTA-Angelus obtained for 1-3 and 3-6 day decreased ALP activity, indicating the presence of inhibitory components in the two MTAs. ProRoot MTA enhanced mRNA levels of DMP-1, but not DSPP. However, the other MTAs did not affect mRNA expression of DSPP, DMP-1, OCN and OPN, which is not consistent with ALP activities. Thus, further studies are needed to conclude the effects of MTA on differentiation of dental pulp cells.
Keywords
mineral trioxide aggregate; cytotoxicity; cyclooxygenase-2; inflammation; differentiation;
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1 E .A. Koulaouzidou, N. Economides, P. Beltes, G. Geromichalos and K. Papazisis, "In vitro evaluation of the cytotoxicity of ProRoot MTA and MTA Angelus," J. Oral Sci., 50, 397-402 (2008).   DOI   ScienceOn
2 S. -J. Ding, C. -T. Kao, C. -L. Chen, M. -Y. Shie and T. -H. Huang, "Evaluation of human osteosarcoma cell line genotoxicity effects of mineral trioxide aggregate and calcium silicate cements," J. Endod., 36, 1158-1162 (2010).   DOI   ScienceOn
3 M. J. B. Sliva, L. Q. Vieira, A. P. R. Sobrinho and B. Horizonte, "The effects of mineral trioxide aggregates on cytokine production by mouse pulp tissue," Oral Surg. Oral Med. Oral Pathol, Oral Radiol. Endod., 105, e70-e76 (2008).   DOI   ScienceOn
4 Y. Yasuda, M. Ogawa, T. Arakawa, T. Kadowaki and T. Saito, "The effects of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells: an in vitro study," J. Endod., 34, 1057-1060 (2008).   DOI   ScienceOn
5 K. Keiser, C. C. Johnson, D. A. Tipton, "Cytotoxicity of mineral trioxide aggregate using human periodontal ligament fibroblasts," J. Endod., 26, 288-291 (2000).   DOI   ScienceOn
6 T. Zhu, B. -S. Lim, H. C. Park, K. M. Son, H. -C. Yang, "Effects of the iron-chelating agent deferoxamine on triethylene glycol dimethacrylate, 2-hydroxylethyl methacrylate, hydrogen peroxideinduced cytotoxicity," J. Biomed. Mater. Res. Part B: Appl. Biomater., 100B, 197-205 (2012).   DOI   ScienceOn
7 N. R. Kim, B. -S. Lim, H. C. Park, K. M. Son and H. -C. Yang, "Effects of N-acetylcysteine on TEGDMA- and HEMA-induced suppression of osteogenic differentiation of human osteosarcoma MG63 cells," J. Biomed. Mater. Res. Part B: Appl. Biomater., 98B, 300-307 (2011).   DOI   ScienceOn
8 N. R. Kim, H. C. Park, I. Kim, B. -S. Lim and H. -C. Yang, "In vitro cytocompatibility of N-acetylcysteine-supplemented dentin bonding agents," J. Endod., 36, 1844-1850 (2010).   DOI   ScienceOn
9 E. -C. Kim, B. -C. Lee, H. -S. Chang, W. Lee, C. -U. Hong and K. -S. Min, "Evaluation of the radiopacity and cytotoxicity of Portland cements containing bismuth oxide," Oral Surg. Oral Med. Oral Pathol, Oral Radiol. Endod., 105, e54-e57 (2008).
10 D. A. Ribeiro, M. M. Sugui, M. A. Matsumoto, M. A. H. Duarte, M. E. A. Marques and D. M. F. Salvadori, "Genotoxicity and cytotoxicity of mineral trioxide aggregate and regular and white Portland cements on Chinese hamster ovary (CHO) cells in vitro," Oral Surg. Oral Med. Oral Pathol, Oral Radiol. Endod., 101, 258-261 (2006).   DOI   ScienceOn
11 S. E. A. Camargo, C. H. R. Camargo, K. -A. Hiller, S. M. Rode, H. Schweikl and G. Schmalz, "Cytotoxicity and genotoxicity of pulp capping materials in two cell lines," Int. Endod. J., 42, 227-237 (2009).   DOI   ScienceOn
12 M. Parirokh, S. Asgary, M. J. Eghbal, S. Kakoei and M. Sammiee, "A Comparative study of using a combination of calcium chloride and mineral trioxide aggregate as the pulp-capping agent on dogs' teeth," J. Endod., 37, 786-788 (2011).   DOI   ScienceOn
13 I. M. Faraco Jr and R. Holland, "Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement," Dent. Traum., 17, 163-166 (2001).   DOI   ScienceOn
14 M. L. R. Accorinte, A. D. Loguercio, A. Reis, J. R. O. Bauer, R. H. M. Grande, S. S. Murata, V. Souza and R. Holland, "Evaluation of two mineral trioxide aggregate compounds as pulp-capping agents in human teeth," Int. Endod. J., 42, 122-128 (2009).   DOI   ScienceOn
15 N. Tani-Ishii, N. Hamada, K. Watanabe, T. Tujimoto, T. Teranaka and T. Umemoto, "Expression of bone extracellular matrix proteins on osteoblast cells in the presence of mineral trioxide," J. Endod., 33, 836-839 (2007).   DOI   ScienceOn
16 H. Perinpanayagam and E. Al-Rabeah, "Osteoblasts interact with MTA surfaces and express Runx2," Oral Surg. Oral Med. Oral Pathol, Oral Radiol. Endod., 107, 590-596 (2009).   DOI   ScienceOn
17 D. Hashiguchi, H. Fukushima, H. Yasuda, W. Masuda, M. Tomikawa, K. Morikawa, K. Maki and E. Jimi, "Mineral trioxide aggregate inhibits osteoclastic bone resorption," J. Dent. Res., 90, 912-917 (2011).   DOI