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Introduction of CAX1 into 'Hongro' Apple via Agrobacterium tumefaciens  

Kim, Jeong-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Kim, Dae-Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Hwang, Jeong Hwan (Fruit Research Division, National Institute of Horticultural & Herbal Science)
Publication Information
Korean Journal of Breeding Science / v.42, no.5, 2010 , pp. 534-539 More about this Journal
Abstract
'Hongro' is early-mid maturing cultivar with good quality like 'Tsugaru' and it has not preharvest drop. The CAX1 gene was introduced into Korean apple cultivar 'Hongro' by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain transgenic apple with enhanced Ca level. The CAX1 gene playing the role of $H^+/Ca^{2+}$ transporter from Arabidopsis thaliana increases Ca concentration in several plants. Regenerated transgenic lines were confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of CAX1 gene. Southern blot analysis of 'Hongro' transformants showed that two putative transgenic lines were integrated with CAX1 gene in genomic DNA. The CAX1 comparative expression levels of two transgenic lines were higher than that of non-transformant evaluated by comparative quantification analysis using a real-time PCR. These two lines were multiplied in vitro, and micro-grafted on apple rootstocks 'M.9' in the isolated greenhouse. Since two years after micro-grafting, the fruits came into bearing. Compared to Ca level of the non-transgenic 'Hongro', that of the CAX1 transgenic 'Hongro' in the flesh and leaves was higher.
Keywords
Agrobacterium tumefaciens; Ca level; Hongro; CAX1;
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