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Changes in Polygalacturonase and Ethylene Biosynthesis of Three Varieties of Apple During Fruit Ripening  

Kim, Se Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Han, Sang Eun (Research Service Division, SolGent. Co., Ltd)
Lee, Hye Eun (Vegetable Research Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Mi-Ae (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Shin, Il Sheob (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Jeong-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Cho, Kang-Hee (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Kim, Dae-Hyun (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Hwang, Jeong Hwan (Fruit Research Division, National Institute of Horticultural & Herbal Science, RDA)
Publication Information
Korean Journal of Breeding Science / v.42, no.5, 2010 , pp. 481-487 More about this Journal
Abstract
The ripening behavior of three apple cultivars, 'Tsugaru', 'Hongro' and 'Fuji' was distinctive and the involvement of POLYGALACTURONASE(PG) in the fruit softening process was confirmed to be ethylene dependent. Fruit softening is genetically coordinated by the action of several cell wall enzymes, including PG which depolymerizes cell wall pectin. Also, loss of firmness is associated with increasing of the ripening hormone, ethylene. In this work, climacteric ripening of three apple cultivars, Tsugaru, Hongro and Fuji, producing different ethylene levels and ripening responses, was examined. Correspondingly in Fuji, a linear and basal ethylene level was observed over the entire period of measurements, and Tsugaru and Hongro displayed a typical climacteric rise in ethylene production. Transcript accumulation of genes involved in ethylene biosynthesis (MdACS3 and MdACO1) and MdPG1 was studied in Tsugaru, Hongro and Fuji cultivars. Expression of MdACO1 transcripts was shown in all three ripened apple fruits. However, the MdACS3 and MdPG1 were transcribed differently in these cultivars. Comparing the MdPG1 of 'Tsugaru', 'Hongro' and 'Fuji', structural difference was discovered by genomic Southern analysis. Overall results pointed out that MdACS3 and MdPG1 play an important role in regulation of fruit ripening in apple cultivar.
Keywords
ACS3 (1-aminocyclopropane-1-carboxylate synthase) gene; ACO1 (1-aminocyclopropane-1-carboxylate oxidase) gene; PG1 (Polygalacturonase) gene; Ethylene pathway; Apple ripening;
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1 Adams DO, Yang SF. 1979. Ethylene biosynthesis: Identification of 1-aminocyclopropane-1-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proc. Natl, Acad. Sci. USA. 76:170-174.   DOI   ScienceOn
2 Aide W, Junko Y, Hisayuki K, Yuhya W, Yoshimichi H, Megumi I, Atsushi K, Tianzhong L, Takeo H. 2009. Null mutation of the MdACS3 gene, coding for a ripeningspecific 1-Aminocyclopropane-1-carboxylate synthase, leads to long shelf life in apple fruit. Plant Physiol. 151:391- 399.   DOI   ScienceOn
3 Atkinson RG, Schroder R, Hallett IC, Cohen D, Macrae EA. 2002. Overexpression of polygalacturonase in transgenic apple trees leads to a range of novel phenotypes involving changes in cell adhesion. Plant Physiol. 129:122-133.   DOI   ScienceOn
4 Barry, CS, Llop-Topus MI, Grierson D. 2000. The regulation of 1-aminocyclopropane-1-carboxylate synthase gene expression during the transition from system-1 to system-2 ethylene synthesis in tomato. Plant Physiol. 123: 979-986.   DOI
5 Brackman A, Streif J. 1994. Ethylene, $CO_2$ and aroma volatiles production by apple cultivars. Acta Hort. 368: 51-57.
6 Crookes PR, Grierson D. 1983 Ultrastructure of tomato fruit ripening and the role of polygalacturonase isoenzymes in cell wall degradation. Plant Physiol. 72:1088-1093.   DOI   ScienceOn
7 Dal Cin V, Rizzini FM, Botton A, Tonutti P. 2006. The ethylene biosynthetic and signal transduction pathways are differently affected by 1-MCP in apple and peach fruit. Postharvest Biol. Technol. 42:125-133.   DOI   ScienceOn
8 DellaPenna D, Lincoln JE, Fischer RL, Bennet AB. 1989. Transcriptional analysis of polygalacturonase and other ripening associated genes in Rutgers, rin, nor and Nr tomato fruit. Plant Physiol 90:1372-1377.   DOI   ScienceOn
9 Dong JG, Kim WT, Yip WK, Thompson GA, Li L, Bennett AB, Yang SF. 1991. Cloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruit. Planta. 185:38-45.
10 Emma T, Hilary I, Kularajathevan G, Sakuntala K, Daisy W, Keith S, Judith B, Ross GA, Jason WJ, Jo P, Roger PH, Robert JS. 2010. The role of ethylene and cold temperature in the regulation of the apple polygalacturonase1 gene and fruit softening. Plant Physiol. 153:294-305.   DOI   ScienceOn
11 Fabrizio C, Sara S, Eric Van de Weg W, Walter G, Michela C, Joseph D, Bernie K, Silviero S. 2005. Role of the genes Md-ACO1 and Md-ACS1 in ethylene production and shelf life of apple (Malus domestica Borkh) Euthytica. 141:181-190.
12 Jim G. 2002. Molecular Biology of Fruit Ripening. Korean Journal of Breeding Science. Poster presentation.
13 Guzman P, Ecker JR. 1990. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell. 2:513-523.
14 Hadfield KA, Bennett AB. 1998. Polygalacturonases: many genes in search of a function. Plant Physiol. 117:337-343.   DOI
15 Harada T, Sunako T, Wakasa Y, Soejima J. Satoh T, Miizeki M. 2000. An allele of the 1-aminocyclopropane- 1-carboxylate synthase gene (Md-ACS1) accounts for the low level of ethylene production in climacteric fruits of some apple cultivars. Theor Appl Genet 101:742-746.   DOI   ScienceOn
16 Jinaguo L, Hong Z, Rongcai Y. 2010. Profiling the expression of genes related to ethylene biosynthesis, ethylene perception and cell wall degradation during fruit abscission and fruit ripening in apple. J. Amer. Soc. Hort. Sci. 135:391-401.
17 Kende H, Zeevaart JAD. 1997. The five "classical" plant hormones. Plant Cell 9:1197-1210.   DOI   ScienceOn
18 Kim WT, Yang SF. 1994. Structure and expression of cDNAs encoding 1-aminocyclopropane-1-carboxylate oxidase homologs isolated from excised mung bean hypocotyls. Planta 194:223-229.   DOI   ScienceOn
19 Lazan H, Ali ZM, Liang AKS, Yee KL. 1989. Polygalacturonase activity and variation in ripening of papaya fruit with tissue depth and heat treatment. Physiol. Plant. 77:93-98.   DOI
20 Lee E, Speirs J, Gray J, Brady CJ. 1990. Homologies to the tomato endopolygalacturonase gene in the peach genome. Plant Cell Environ. 13:513-521.   DOI
21 Pressey R, Avants JK. 1976. Pear polygalacturonases. Phytochemistry 15:1349-1351.   DOI   ScienceOn
22 Rosenfield C, Kiss E, Hrazdina G. 1996. MdACS-2(Accession No. U73815) and MdACS-3 (Accession No. U73816): Two new 1-aminocyclopropane-1-carboxylate synthase in ripening apple fruit (PGR96-122) Plant Physiol. 112:1735.   DOI
23 Lodhi MA, Ning Y, Weeden NF, Reisch BI. 1994. A simple and efficient method for DNA extraction from grapevine cultivars, Vitis species and Ampelopsis. Plant Mol. Biol. Rep. 12:6-13.   DOI   ScienceOn
24 Peiser G, Fa YS. 1998. Evidence for 1-(Malonylamino) cyclopropane-1-carboxylic acid being the major conjugate of aminocyclopropane-1-carboxylic acid in tomato fruit. Plant Physiol. 116:1527-1532.   DOI
25 Phoebe RJ, Joseph RE. 1998. The ethylene gas signal transduction pathway: A molecular perspective. Annu. Rev. Genet. 32:227-254.   DOI   ScienceOn
26 Ross GA, Karen MB, Michele AW, Teresa I-B, Suzanne JR, Gavin SR. 1998. Apple ACC-oxidase and polygalacturonase: ripening-specific gene expression and promoter analysis in transgenic tomato. Plant Molecular Biology. 38:449-460.   DOI   ScienceOn
27 Ross GA, Roswitha S, Ian CH, Daniel C, Elspeth AM. 2002. Overexpression of polygalacturonase in transgenic apple trees leads to a range of novel phenotypes involving changes in cell adhesion Plant Physiol. 129:122-133.   DOI   ScienceOn
28 Shujun C, Jeff P, John C. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Molecular Biology Reproter. 11:113-116.   DOI   ScienceOn
29 Summers JE, Voesenek LACJ, Blom CWPM, Lewis MJ, Jackson MB. 1996. Potamogeton pectinatus is constitutively incapable of synthesizing ethylene and lacks 1-aminocyclopropane- 1-carboxylic acid oxidase. Plant Physiol. 111: 901-908.
30 Sunako T, Sakuraba W, Senda M, Akada S, Ishikawa R, Niiseki M, Harada T. 1999. An allele of the ripeningspecific 1-amino-cyclopropane-1carboxylic synthase gene (ACS1) in apple fruit with a long storage life. Plant Physiol. 119:1297-1303.   DOI
31 Wakasa Y, Kudo H, Ishikawa R, Akada S, Senda M, Niizeki M, Harada T. 2006. Low expression of an endopolygalacturonase gene in apple fruit with long-term storage potential. Postharvest Biol. Technol. 39:193-198.   DOI   ScienceOn
32 Sunako, T, Ishikawa R, Senda M, Akada S, Niizeki M, Harada T. 2000. MdACS-5A (Accession No. AB034992) and 5B (Accession No. AB034993), two wound-responsive genes encoding 1-aminocyclopropane-1-carboxylate synthase in apple. (PGR00030) Plant Physiol. 112:620.
33 Theologis A. 1992. One rotten apple spoils the whole bushel: the role of ethylene in fruit ripening. Cell 70:181- 184.   DOI   ScienceOn
34 Vogen JP, Woeste KE, Theologis A, Kieber JJ. 1998. Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confercytokinin-insensitivity and ethylene overproduction respectively. Proc. Natl. Acad. Sci. USA 8:4766-4771.
35 Wang Z-Y, Macrae EA, Wright MA, Bolitho KM, Ross GS, Atkinson RG. 2000. Polygalacturonase gene expression in kiwifruit: relationship to fruit softening and ethylene production. Plant Mol Biol. 42:317-328.   DOI   ScienceOn