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Differentially Expressed Genes Related to Cold-resistance in Barley (Hordeum vulgare L. cv. Nagaoka)  

Chun, Jong Un (Dept. of Well-being Resources, Sunchon National University)
Park, Jeong-Seon (Dept. of Well-being Resources, Sunchon National University)
Bae, Chang-Hyu (Dept. of Well-being Resources, Sunchon National University)
Shin, Jeong-Sheop (School of Life Sciences and Biotechnology, Korea University)
Publication Information
Korean Journal of Breeding Science / v.41, no.2, 2009 , pp. 73-83 More about this Journal
Abstract
To investigate genes related to vernalization and cold- resistance in barley (Hordeum vulgare L. cv. Nagaoka), differentially expressed genes were identified from cold-resistant barley leaves with suppression subtractive hybridization (SSH) and Northern blot analyses. The nucleotide and the deduced amino acid sequences of the putative gene products were compared. The bvrn-7 showed high homology(84%) with gene related to vernalization, and the bvrn-3, bvrn-12, bvrn-28, bvrn-29 and bvrn-36 related to cold-resistant genes had high identity of 88~98% with low temperature-induced genes. The results indicate that the 6 genes were closely related to vernalization and cold-resistance during low temperature treatment.
Keywords
suppression subtractive hybridization (SSH); barley; vernalization; cold-resistant gene;
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