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Genetic Diversity and Discrimination of Recently Distributed Korean Cultivars by SSR Markers  

Sun, Ming-Mao (Department of Crop Science, Chungbuk National University)
Choi, Keun-Jin (Korea Seed & Variety Service)
Kim, Hong-Sig (Department of Crop Science, Chungbuk National University)
Song, Beom-Heon (Department of Crop Science, Chungbuk National University)
Woo, Sun-Hee (Department of Crop Science, Chungbuk National University)
Lee, Chul-Won (Department of Crop Science, Chungbuk National University)
Jong, Seung-Keun (Department of Crop Science, Chungbuk National University)
Cho, Yong-Gu (Department of Crop Science, Chungbuk National University)
Publication Information
Korean Journal of Breeding Science / v.41, no.2, 2009 , pp. 115-125 More about this Journal
Abstract
This study was undertaken to investigate the genetic diversity and to develop a technique for cultivar discrimination using SSR markers in rice. Sixty-seven recently distributed rice cultivars in Korea from 1998 to 2005 were evaluated by 20 SSR markers. A total of 149 alleles were produced ranging from 4 to 14 alleles with an average of 7.5 alleles per locus. The molecular weight of alleles per locus varied from 4 bp (RM253) to 51 bp (RM335), and PIC values ranged from 0.45 (RM202) to 0.87 (RM204) with an average of 0.67. Of them, seven markers, RM204, RM257, RM21, RM224, RM249, RM253, and RM264, were selected as key markers for differentiating rice varieties. The seven markers produced a total of 67 alleles with an average of 9.6 alleles per marker. PIC values ranged from 0.48 (RM253) to 0.87 (RM204) with an average of 0.72. The 63 cultivars (94%) out of 67 cultivars could be individually identified by the genotype using the seven SSR markers, which will be applicable to discriminating rice cultivars.
Keywords
cultivar; discrimination; SSR; rice;
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