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Disease Reaction of a Japonica Rice, Keumo3, and Detection of a Linked DNA Marker to Leaf Blast Resistance  

Lee, Jong-Hee (Department of Functional Crop Science, NICS, RDA)
Kwak, Do-Yeon (National Institute of Crop Science, RDA)
Pakr, Dong-Soo (Department of Functional Crop Science, NICS, RDA)
Roh, Jae-Hwan (National Institute of Crop Science, RDA)
Kang, Jong-Rae (Department of Functional Crop Science, NICS, RDA)
Kim, Choon-Song (Department of Functional Crop Science, NICS, RDA)
Jeon, Myeong-Gi (Department of Functional Crop Science, NICS, RDA)
Yeo, Un-Sang (Department of Functional Crop Science, NICS, RDA)
Yi, Gihwan (Department of Functional Crop Science, NICS, RDA)
Shin, Mun-Sik (Department of Rice and Winter Cereal Crop Science, NICS, RDA)
Oh, Byeong-Geun (Department of Functional Crop Science, NICS, RDA)
Hwang, Hung-Goo (Department of Rice and Winter Cereal Crop Science, NICS, RDA)
Publication Information
Korean Journal of Breeding Science / v.40, no.4, 2008 , pp. 408-413 More about this Journal
Abstract
Rice blast resistance is considered one of the most important traits in rice breeding and the disease, caused by Magnaporthe grisea Barr, has brought significant crop losses annually. Moreover, breakdown of resistance normally occurs in two to five years after cultivar release, thus a more durable resistance is needed for better control of this disease. We developed a new variety, Keumo3, which showed strong resistance to leaf blast. It was tested in 2003 to 2007 at fourteen blast nursery sites covering entire rice-growing regions of South Korea. It showed resistance reactions in 12 regions and moderate in 2 regions without showing susceptible reactions. Durability test by sequential planting method indicated that this variety had better resistance. Results showed that Keumo3 was incompatible against 19 blast isolates with the exception of KI101 by artificial inoculation. To understand the genetic control of blast resistance in rice cultivar Keumo3 and facilitate its utilization, recombinant inbred lines (RIL) consisting of 290 F5 lines derived from Akidagomachi/Keumo3 were analyzed and genotyped with Pizt InDel marker zt56591. The recombination value between the marker allele of zt56591 and bioassay data of blast nursery test was 1.1%. These results indicated that MAS can be applied in selecting breeding populations for blast resistance using zt56591 as DNA marker.
Keywords
Keumo3; Leaf blast; Marker assisted selection (MAS); Rice;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Liu G, Lu G, Zeng L, Wang GL. 2002. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol. Genet. Genomics 267: 472-480   DOI
2 Cho YC, Kwon SW, Choi IS, Lee SK , Jeon JS, Oh MK, Roh JH, Hwang HG, Yang SJ, Kim YG. 2007. Identification of major blast resistance genes in korean rice varieties (Oryza sativa L.) using molecular markers. J. Crop. Biothch. Sci: 265-276
3 Kim KH, Kim DH, Cho YH. 2001. Development of near isogenic lines and genetic analysis for amylose content of rice grain. Korean J. Breed Sci. 33(4):280-286
4 Han SS, Ra DS, Kim CK. 1995. Incidence of panicle blast, rice distribution during 1993-1994 and pathogenicity of new races of Pyricularia grisea in Korea. Korean J. Plant Pathol. 11(3):238-244   과학기술학회마을
5 Jin X, Lee EJ, Choi JE. 2007. Classification of korean rice cultivars based on reaction pattern to japanese isolates of blast pathogen J. Crop Sci. Biotech. 10:5-9
6 Kim DY, Shim HS, Choi SH, Lei C, Ling ZZ, Han SS. 2004. Comparative assay fungus population and resistant genes about Magnaporthe grisea between Korea and China. Res. Plant Dis. 10(4):217-226   DOI
7 Ryu JD, Yeh WH, Han SS, Lee YH, Lee EJ. 1987. Regional and annual fluctuation of races of Pyricularia oryzae during 1977-1985 in Korea. Korean J. Plant Pathol. 3: 174-179   과학기술학회마을
8 Hayashi K, Yoshida H, Ashikawa I. 2006. Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes. Theor. Appl. Genet. 113(2) :251-260   DOI
9 Roh JH, Cho YC, Kim YG, Oh IS, Lee JY, Han SS. 2007. Blast resistance of high quality rice cultivars Korean J. Bred. Sci. 39(3):288-295
10 Bonman JM, Khush GS, Nelson RJ. 1992. Breeding Rice for Resistance to Pests Annu. Rev. in Phytopathol. 30:507-528   DOI   ScienceOn
11 Jeung JU, Kim BR, Cho YC, Han SS, Moon HP, Lee YT, Jena KK. 2007. A novel gene, Pi40(t), linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice. Theor Appl Genet. 115 (8):1163-1177   DOI
12 Han SS, Ryu JD, Shim HS, Lee SW, Hong YK, Cha KH. 2001. Breakdown of resistance of rice cultivars by new race KI-1117a and Race distribution of rice blast fungus during 1999-2000 in Korea. Rec. Plant Dis (2):86-92
13 Lee EW, and Park SZ. 1979. Interpretation on the epidemic outbreak of rice blast disease in Korea, 1978. J. Korean Soc. Crop Sci. 24(1):1-10
14 Ra DS, Han SS, Kim CK. 1994. Temporal and spatial blast incidence in leading cultivars and elite lines of rice in Korea. Korean J. Plant Pathol. 10(1):47-53
15 Kim BR, Roh JH, Choi SH Ahn SW, Han SS. 2004. Durability of rice cultivars to blast in Korea by sequential planting method. Korean J. Bred. Sci. 36(5):350-356