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http://dx.doi.org/10.22034/APJCP.2016.17.10.4665

Evaluating the Existence of Small Compressed Binucleated Squamous Cells in ASC-H  

Okodo, Mitsuaki (Department of Medical Technology, Kyorin University, Faculty of Health Sciences)
Okayama, Kaori (School of Medical Technology, Faculty of Health Science, Gumma Paz College)
Kitamura, Hiroshi (Department of Medical Oncology, Kyorin University School of Medicine)
Shiina, Natsuko (ILABO Cyto STD Laboratory, Inc)
Caniz, Timothy (ILABO Cyto STD Laboratory, Inc)
Ono, Midori (ILABO Cyto STD Laboratory, Inc)
Yabusaki, Hiromi (ILABO Cyto STD Laboratory, Inc)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.17, no.10, 2016 , pp. 4665-4669 More about this Journal
Abstract
Purpose: To evaluate the legitimacy of a diagnosis of ASC-H in 5 cases which were followed up monthly for over 2 years with both cytology and HPV testing. Methods: Some 5 cases out of a total of 25.0 self-sampled Pap test patients diagnosed as ASC-H provided 119 specimens over 2 years, with HPV-DNA testing perormed using a E6 primer. Results: Cases 1, 2 and 3 showed SIL after the ASC-H diagnosis, while cases 4 and 5 showed and maintained NILM. Cases 1, 2 and 3 were further characterized by small atypical compressed binucleated cells, in which HPV was detected by in situ PCR. Case 4 showed a high N/C ratio in cells in sheets with a mild increase in chromatin. Case 5 demonstrated a high N/C ratio in small cells with no increase in chromatin. Conclusion: The finding of a compressed binucleated cells can define the difference between degenerated endocervical columnar cells and small atypical cells suggestive of HSIL. When small compressed binucleated squamous cells are detected, there may be a chance of continuing HPV infection and undetected SIL.
Keywords
HPV; ASC-H; compressed binucleated squamous cells;
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1 Louro AP, Roberson J, Eltoum I, Chhieng DC (2003). Atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion. A follow-up study of conventional and liquid-based preparations in a high-risk population. Am J Clin Pathol, 120, 392-7.   DOI
2 Louro AP, Roberson J, Eltoum I, Chhieng DC (2013). Atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesion. Am J Clin Pathol, 120, 392-97.
3 Okayama K, Okodo M, Kumagai T, et al (2010a). Improvement in situ PCR -Detection of single-copy HPV-DNA. J Jpn Soc Clin Cytol, 49, 393-9.   DOI
4 Okayama K, Okodo M, Fujii M, et al (2010b). New cell findings on low-grade squamous intra epithelial lesions (LSIL)-minimizing the atypical squamous sells of undetermined significance (ASC-US) diagnosis rate. J Jpn Soc Clin Cytol, 49, 321-9.   DOI
5 Okayama K, Okodo M, Kumagai T, et al (2012). Improved accuracy of cytodiagnosis using the Kato self-collection devise: the usefulness of smear preparation in liquid-based cytology methods. Asian Pac J Cancer Prev, 13, 4521-24.   DOI
6 Okayama K, Okodo M, Kumagai T, et al (2013). Re-examination of the cell findings of HPV infection-The presence or absence of HPV-DNA. J Jpn Soc Clin Cytol, 52, 224-30.   DOI
7 Quddus MR, Sung CJ, Steinhoff MM, et al (2001). Atypical squamous metaplastic cells. Cancer Cytopathol, 93, 16-22.   DOI
8 Schoolland M, Sterrett GF, Knowles SA, et al (1998). The "Inconclusive-possible high grade epithelial abnormality" category in Papanicolaou smear reporting. Cancer, 84, 208-17.   DOI
9 Selvaggi SM (2003). Reporting of atypical squamous cells, cannot exclude a high-grade squamous intraepithelial lesion (ASC-H) on cervical samples: is it significant? Diagn Cytopathol, 29, 38-41.   DOI
10 Sheils LA, Wilbur DC (1997). Atypical squamous cells of undetermined significance. Stratification of the risk of association with, or progression to, squamous intraepithelial lesions based on morphologic subcategorization. Acta Cytol, 41, 1065-72.   DOI
11 Sherman ME, Solomon D, Schiffman M (2001). ASCUS LSIL triage study group. qualification of ASCUS. a comparison of equivocal LSIL and equivocal HSIL cervical cytology in the ASCUS LSIL Triage Study. Am J Clin Pathol, 116, 386-94.   DOI
12 Sherman ME, Castle PE, Solomon D (2006). Cervical cytology of atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesion (ASC-H). Cancer cytopathol, 108, 298-305.   DOI
13 Simsir A, Ioffe O, Sun P, et al (2006). Effect of Bethesda 2001 on reporting of atypical squamous cells (ASC) with special emphasis on atypical squamous cells-cannot rule out high grade (ASC-H). Diagn Cytopathol, 34, 62-6.   DOI
14 Solomon D, Davey D, Kurman R, et al (2002). The 2001 Bethesda System: terminology for reporting results of cervical cytology. JAMA, 287, 2114-9. ----   DOI
15 Alli PM, Ali SZ (2003). Atypical squamous cells of undetermined significance-rule out high-grade squamous intraepithelial lesion: cytopathologic characteristics and clinical correlates. Diagn Cytopathol, 28, 308-12.   DOI