Browse > Article
http://dx.doi.org/10.7314/APJCP.2015.16.2.761

Wild Carrot Oil Extract is Selectively Cytotoxic to Human Acute Myeloid Leukemia Cells  

Tawil, Mirna (Department of Natural Sciences, School of Arts and Sciences, Lebanese American University)
Bekdash, Amira (Department of Natural Sciences, School of Arts and Sciences, Lebanese American University)
Mroueh, Mohammad (Department of Pharmaceutical Sciences, School of Pharmacy, Lebanese American University)
Daher, Costantine F. (Department of Natural Sciences, School of Arts and Sciences, Lebanese American University)
Abi-Habib, Ralph J. (Department of Natural Sciences, School of Arts and Sciences, Lebanese American University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.2, 2015 , pp. 761-767 More about this Journal
Abstract
Background: In this study, we used Daucus carota oil extract (DCOE) to target acute myeloid leukemia (AML) cells. All the AML cell lines tested were sensitive to the extract while peripheral mononuclear cells were not. Analysis of mechanism of cell death showed an increase in cells positive for annexinV and for active caspases, indicating that DCOE induces apoptotic cell death in AML. Inhibition of the MAPK pathway decreased sensitivity of AML cells to DCOE, indicating that cytotoxicity may be dependent on its activity. In conclusion, DCOE induces selective apoptosis in AML cells, possibly through a MAPK-dependent mechanism.
Keywords
AML; Daucus carota; apoptosis; MAPK;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Abi-Habib RJ, Liu S, Bugge T, Leppla SH, Frankel AE (2004). A urokinase-activated recombinant diphtheria toxin targeting the granulocyte-macrophage colony-stimulating factor receptor is selectively cytotoxic to human acute myeloid leukemia blasts. Blood, 104, 2143-48.   DOI
2 Abi-Habib RJ, Urieto JO, Liu SH, et al (2005). Braf status and mitogen-activated protein/extracellular signal-regulated kinase kinase 1/2 activity indicate sensitivity of melanoma cells to anthrax lethal toxin. Mol Cancer Ther, 4, 1303-10.   DOI
3 Akgul Y, Aktas LY, Anil H (2009). Compounds from flowers of Daucus carota L. SSP. carota and their antioxidant activity. Chem Nat Compd, 45, 889-92.   DOI
4 Alachkar H, Santhanam R, Harb JG, et al (2013). Silvestrol exhibits significant in vivo and in vitro antileukemic activities and inhibits FLT3 and miR-155 expressions in acute myeloid leukemia. J Hematol Oncol, 6, 21.   DOI   ScienceOn
5 American Cancer Society: Cancer Facts and Figures 2012. Atlanta, Ga: American Cancer Society.
6 Asmaa M, Al-Jamal H, Ang C, et al (2014). Apoptosis induction in MV4-11 and K562 human leukemic cells by Pereskia sacharosa (Cactaceae) leaf crude extract. Asian Pac J Cancer Prev, 15, 475-81.   DOI   ScienceOn
7 Barnes J (1998). Herbal medicine. Pharm J, 260, 344-8.
8 Bates DJ, Danilov AV, Lowrey CH, Eastman A (2013). Vinblastine rapidly induces NOXA and acutely sensitizes primary chronic lymphocytic leukemia cells to ABT-737. Mol Cancer Ther, 12, 1504-14.   DOI
9 Bennett JM, Kouides PA, Forman SJ (2002). The myelodysplastic syndromes: morphology, risk assessment, and clinical management. Int J Hematol, 2, 228-38.
10 Cuendet M, Christov K, Lantvit DD, et al (2004). Multiple myeloma regression mediated by bruceantin. Clin Cancer Res, 10, 1170-9.   DOI
11 Gupta K, Chakrabarti A, Rana S, et al (2011). Securinine, a myeloid differentiation agent with therapeutic potential for AML. PLoS One, 6, 21203.   DOI
12 Hoffman D (1990). The new holistic herbal (3rdedn). Element Pres, Shaftesbury Dorset: Great Britain.
13 Kang SH, Jeong SJ, Kim SH, et al (2012). Icariside II induces apoptosis in U937 acute myeloid leukemia cells: role of inactivation of STAT3-related signaling. PLoS One, 7, 28706.   DOI
14 Kassab E, Darwish M, Timsah Z, et al (2013). Cytotoxicity of anthrax lethal toxin to human acute myeloid leukemia cells is non-apoptotic and dependent on extracellular signal-regulated kinase 1/2 activity. Transl oncol, 6, 25-32.   DOI
15 Nelson DM, Joseph B, Hillion J, et al (2011). Flavopiridol induces BCL-2 expression and represses oncogenic transcription factors in leukemic blasts from adults with refractory acute myeloid leukemia. Leuk Lymphoma, 52, 1999-2006.   DOI
16 Kma L (2014). Plant extracts and plant-derived compounds: promising players in countermeasure strategy against radiological exposure: a review. Asian Pac J Cancer Prev, 15, 2405-25.   DOI
17 Madan J, Pandey RS, Jain V, et al (2013). Poly (ethylene)-glycol conjugated solid lipid nanoparticles of noscapine improve biological half-life, brain delivery and efficacy in glioblastoma cells. Nanomedicine, 9, 492-503.   DOI
18 Maxia A, Marongiu B, Piras A, et al (2009). Chemical characterization and biological activity of essential oils from Daucus carota L. subsp. carota growing wild on the Mediterranean coast and on the Atlantic coast. Fitoterapia, 80, 57-61.   DOI
19 Omoregie SN, Omoruyi FO, Wright VF, Jones L, Zimba PV (2013). Antiproliferative activities of lesser galangal (Alpinia officinarum Hance Jam1), Turmeric (Curcuma longa L.), and Ginger (Zingiber officinale Rosc.) against acute monocytic leukemia. J Med Food, 16, 647-55.   DOI
20 Ramkumar KM, Manjula C, Elango B, et al (2013). In vitro cytotoxicity of Gymnema montanum in human leukaemia HL-60 cells; induction of apoptosis by mitochondrial membrane potential collapse. Cell Prolif, 46, 263-71.   DOI
21 Rossi P-G, Bao L, Luciani A, et al (2007). (E)-methylisoeugenol and elemicin: antibacterial components of Daucus carota L. essential oils against campylobacter jejuni. J Agric Food Chem, 55, 7332-6.   DOI
22 Shebaby WN, El-Sibai M, Smith KB, et al (2013). The antioxidant and anticancer effects of wild carrot oil extract. Phytother Res, 27, 737-44.   DOI   ScienceOn
23 Trifilio S, Zhou Z, Altman J, et al (2013). Dose-intense etoposidecyclophosphamide without stem cell transplantation for patients with intermediate and high cytogenetic risk primary refractory and relapsed acute myeloid leukemia. Leuk Res, 37, 872-6.   DOI
24 Staniszewska M, Kula J, Wieczorkiewicz M, Kusewicz D (2005). Essential oils of wild and cultivated carrots - the chemical composition and antimicrobial activity. J Essent Oil Res, 17, 579-83.   DOI
25 Tanios R, Bekdash A, Kassab E, et al (2013). Human recombinant arginase I(Co)-PEG5000 [HuArgI(Co)-PEG5000]-induced arginine depletion is selectively cytotoxic to human acute myeloid leukemia cells. Leuk Res, 37, 1565-71.   DOI
26 Thomas KJ, Nicholl JP, Coleman P (2001). Use and expenditure on complementary medicine in England: a population based survey. Complement Ther Med, 9, 2-11.   DOI
27 Van Wyk B-E, Wink M. 2004. Medicinal plants of the world. Briza Publications: Pretoria, South Africa.
28 Watanabe R, Tomita N, Kishimoto K, et al (2013). Absolute monocyte count in follicular lymphoma patients treated with rituximab plus cyclophosphamide, doxorubicin, vincristine, and prednisone. Leuk Res, 37, 1208-12.   DOI
29 Wehbe K, Mroueh M, Daher C (2009). The potential role of Daucus carota aqueous and methanolic extracts on inflammation and gastric ulcers in rats. J Comp Integ Med, 6, 16.
30 Wei Fu H, Zhang L, Yi T, Lin Feng Y, Kui Tian J (2009). A new sesquiterpene from the fruits of Daucus carota L. Molecules, 14:2862-2867.   DOI
31 Zeinab RA, Mroueh M, Diab-Assaf M, et al (2011). Chemopreventive effects of wild carrot oil against 7,12-Dimethyl Benz(a)anthracene-Induced Squamous Cell Carcinoma in Mice. Pharm Biol, 49, 955-61.   DOI