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http://dx.doi.org/10.7314/APJCP.2015.16.13.5325

Anti-proliferative and Apoptotic Effects of Dendrosomal Farnesiferol C on Gastric Cancer Cells  

Aas, Zohreh (Department of Biology, School of Natural Sciences, University of Tabriz)
Babaei, Esmaeil (Department of Biology, School of Natural Sciences, University of Tabriz)
Feizi, Mohammad Ali Hosseinpour (Department of Biology, School of Natural Sciences, University of Tabriz)
Dehghan, Gholamreza (Department of Biology, School of Natural Sciences, University of Tabriz)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.13, 2015 , pp. 5325-5329 More about this Journal
Abstract
Farnesiferol C is a natural compound with various anti-cancer properties that belongs to the class of sesquiterpene coumarins. However, the low bioavailability of farnesiferol C limits its therapeutic potential. Here, we overcame this problem utilizing dendrosome nano-particles and evaluated the anti-cancer effect of dendrosomal farnesiferol C (DFC) on the AGS gastric cancer cell line. The 3-(4,5-dimethyl-thiazol-2yl)-2,5- diphenyl tetrazolium bromide (MTT) assay and reverse transcriptase-polymerase chain reaction (RT-PCR) were respectively used to detect the anti-proliferative properties of DFC and expression ratio of Bax/Bcl-2 as a hallmark of apoptosis. Compared to the void farnesiferol C (FC), our data showed that DFC significantly suppresses the proliferation of AGS cells in a time- and dose-dependent manner (P<0.01). Also, DFC meaningfully increased the expression ratio of Bax/Bcl-2 in AGS cells (P<0.01). The findings demonstrate that our nano-based formulation of farnesiferol C could be considered as a potential therapeutic agent in cancer targeting.
Keywords
AGS cell line; Bax/Bcl-2; coumarin; dendrosome; phytochemicals;
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