Browse > Article
http://dx.doi.org/10.7314/APJCP.2015.16.12.5025

Interferon-γ and Interleukin-10 Gene Polymorphisms are not Predictors of Chronic Hepatitis C (Genotype-4) Disease Progression  

Bahgat, Nermine Ahmed (Clinical and Chemical Pathology Department, Faculty of medicine, Cairo University)
Kamal, Manal Mohamed (Clinical and Chemical Pathology Department, Faculty of medicine, Cairo University)
Abdelaziz, Ashraf Omar (Endemic medicine and Hepatogastroenterology Department, Faculty of Medicine, Cairo University)
Mohye, Mohamed Ahmed (Endemic medicine and Hepatogastroenterology Department, Faculty of Medicine, Cairo University)
Shousha, Hend Ibrahim (Endemic medicine and Hepatogastroenterology Department, Faculty of Medicine, Cairo University)
ahmed, Mae Mohamed (Clinical and Chemical Pathology Department, Faculty of medicine, Cairo University)
Elbaz, Tamer Mahmoud (Endemic medicine and Hepatogastroenterology Department, Faculty of Medicine, Cairo University)
Nabil, Mohamed Mahmoud (Endemic medicine and Hepatogastroenterology Department, Faculty of Medicine, Cairo University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.12, 2015 , pp. 5025-5030 More about this Journal
Abstract
Immunoregulatory cytokines have an influence on hepatitis C virus (HCV) infection outcome. This study aimed to determine whether single nucleotide polymorphisms (SNP) in IFN- ${\gamma}$ and IL-10 genes are associated with susceptibility and/or are markers of prognosis regarding chronic hepatitis C outcomes. IFN ${\gamma}$ (+874T/A) and IL-10 (-1082G/A) genotypes were determined in 75 HCV genotype 4 patients with different disease severities (chronic hepatitis, n=25, liver cirrhosis and hepatocellular carcinoma (HCC) on top of liver cirrhosis, n=50) and 25 healthy participants using allele-specific polymerase chain reaction. No statistical differences in allele or genotype distributions of IFN ${\gamma}$ and IL-10 genes were detected between patients and controls or between patientgroups. No significant difference in the frequency of IL-10 SNP at position -1082 or IFN-${\gamma}$ at position +874T/A was found between chronic HCV genotype 4 and with progression of disease severity in liver cirrhosis or HCC. In conclusion; interferon-${\gamma}$ and interleukin-10 gene polymorphisms are not predictors of disease progression in patients with chronic hepatitis C (Genotype-4).
Keywords
Chronic hepatitis C; single nucleotide polymorphism; IFN-${\gamma}$; IL-10; HCV genotype 4;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Barrett S, Collins M, Kenny C, et al (2003). Polymorphisms in tumour necrosis factor-alpha, transforming growth factor-beta, interleukin-10, interleukin-6, interferon-gamma, and outcome of hepatitis C virus infection. J Med Virol, 71, 212-8.   DOI
2 Bei CH, Bai H, Yu HP, et al (2014). Combined effects of six cytokine gene polymorphisms and SNP-SNP interactions on hepatocellular carcinoma risk in Southern Guangxi, China. Asian Pac J Cancer Prev, 15, 6961-7.   DOI
3 Bouzgarrou N, Hassen E, Farhat K, et al (2009). Combined analysis of interferon-${\gamma}$ and interleukin-10 gene polymorphisms and chronic hepatitis C severity. Human Immunology, 230, 236-70.
4 Bruix J, Sherman M (2011). American association for the study of liver diseases. management of hepatocellular carcinoma: an update. Hepatology, 53, 1020-22.   DOI
5 Chuang JY, Yang SS, Lu YT, et al (2009). IL-10 promoter gene polymorphisms and sustained response to combination therapy in Taiwanese chronic hepatitis C patients. Dig Liver Dis, 41, 424-30.   DOI
6 Dai CY, Chuang WL, Hsieh MY, et al (2006). Polymorphism of interferon-gamma gene at position +874 and clinical characteristics of chronic hepatitis C. Transl Res, 148, 128-33.   DOI
7 Duan Y, Shi JN, Pan C, Chen HL, Zhang SZ (2014) Association between the interleukin-17A -197G>A (rs2275913) polymorphism and risk of digestive cancer. Asian Pac J Cancer Prev, 15, 9295-300.   DOI
8 Galal IF, Zakaria Z, Allam WR, et al (2014). Cross reactive cellular immune response to HCV genotype 1 and 4 antigens among genotype 4 exposed subjects. PLoS One, 9, 101264.   DOI
9 Gao QJ, Liu DW, Zhang SY, Jia M, Wu LH (2010). Association between IFN-gamma+874 polymorphisms and the clinical outcomes of hepatitis B and/or hepatitis C virus infection. Zhonghua Liu Xing Bing XueZaZhi, 31, 324-8.
10 Gigi E, Raptopoulou-Gigi M, et al (2008). Cytokine mRNA expression in hepatitis C virus infection: Th1 predominance in patients with chronic hepatitis C and Th1-Th2 cytokine profile in subjects with self-limited disease. J Viral Hepat, 15, 145-54.
11 Grakoui A, Shoukry NH, Woollard DJ, et al (2003). HCV persistence and immune evasion in the absence of memory T-cell help. Science, 302, 659-62.   DOI
12 Guerra J, Garenne M, Mohamed MK, Fontanet A (2012). HCV burden of infection in Egypt: results from a nationwide survey. J Viral Hepat, 19, 560-7.   DOI
13 Helal SF, Gomaa HE, Thabet EH, Younan MA, HelmyNA (2014). Impact of IL-10 (-1082) promoter-single nucleotide polymorphism on the outcome of hepatitis C virus genotype 4 infection. Clin Med Insights Gastroenterol, 1, 19-24.
14 Heydtmann M, Adams DH (2009). Chemokines in the Immunopathogenesis of Hepatitis C Infection. Hepatology, 49, 676-88.   DOI
15 Kaplan DE, Sugimoto K, Newton K, et al (2007). Discordant role of CD4 T-cell response relative to neutralizing antibody and CD8 T-cell responses in acute hepatitis C. Gastroenterology, 132, 654-66.   DOI   ScienceOn
16 Knapp S, Hennig BJ, Frodsham AJ, et al (2003). Interlleukin-10 promoter polymorphisms and the outcome of hepatitis C virus infection. Immunogenetics, 55, 362-9.   DOI
17 Kusumoto K, Uto H, Hayashi K, et al (2006). Interleukin-10 or tumor necrosis factor-alpha polymorphisms and the natural course of hepatitis C virus infection in a hyperendemic area of Japan. Cytokine, 34, 24-31.   DOI
18 Ollier WE (2004). Cytokine genes and disease susceptibility. Cytokine, 28, 174-8.   DOI
19 Miller SA, Dykes DD, Polesky HF (1988). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res, 16, 1215.   DOI
20 Mullighan CG, Marshall SE, Bunce M, Welsh KI (1999). Variation in immunoregulatory genes determines the clinical phenotype of common variable immunodeficiency.GenesImmun, 1, 137-48.
21 Pravica V, Perrey C, Stevens A, LeeJ H, Hutchinson IV (2000). A single nucleotide polymorphism in the first intron of the human IFN gamma gene: Absolute correlation with a polymorphic CA microsatellite marker of high IFN gamma production. Hum Immunol, 61, 863-6.   DOI
22 Sarvari J, NorozianH, FattahiMR, PirbonyehN, Moattari A (2014). The role of interferon gamma gene polymorphism (+874A/T, +2109A/G, and -183G/T) in response to treatment among hepatitis C infected patients in fars province, Southern Iran. Hepat Mon, 14, 14476.
23 Swi.tek BJ (2012). Is interleukin-10 gene polymorphism a predictive marker in HCV infection? Cytokine Growth Factor Rev, 23, 47-59.   DOI
24 Vidigal PG, Germer JJ, Zein NN (2002). Polymorphisms in the interleukin-10, tumor necrosis factor-alpha and transforming growth factor-beta 1 genes in chronic hepatitis C patients treated with interferon and ribavirin. J Hepatol, 36, 271-7.
25 Wu LM, Zhou L, Xu J, et al (2013). Lack of association between genetic polymorphisms in cytokine genes and tumor recurrence in patients with hepatocellular carcinoma undergoing transplantation. Hepatobiliary Pancreat Dis Int, 12, 54-9.   DOI
26 Asahina Y, Tsuchiya K, Nishimura T, et al (2013). $\alpha$-fetoprotein levels after interferon therapy and risk of hepatocarcinogenesis in chronic hepatitis C. Hepatology, 58, 1253-62.   DOI
27 Xiao YS, Gao Q, Xu XN, et al (2013). Combination of intratumoral invariant natural killer T cells and interferon-gamma is associated with prognosis of hepatocellular carcinoma after curative resection. PLoS One, 8, 70345.   DOI
28 Zekri Ael-R, Nassar AA, El-Din El-Rouby MN, et al (2014). Disease progression from chronic hepatitis C to cirrhosis and hepatocellular carcinoma is associated with increasing DNA promoter methylation. Asian Pac J Cancer Prev, 14, 6721-6.
29 Abbas Z, Moatter T, Hussainy A, Jafri W (2005). Effect of cytokine gene polymorphism on histological activity index, viral load and response to treatment in patients with chronic hepatitis C genotype 3. World J Gastroenterol, 11, 6656-61.   DOI
30 Al-Kubaisy WA, Obaid KJ, Noor NA, Ibrahim NS, Al-Azawi AA (2014). Hepatitis C virus prevalence and genotyping among hepatocellular carcinoma patients in Baghdad. Asian Pac J Cancer Prev, 15, 7725-30.   DOI