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http://dx.doi.org/10.7314/APJCP.2015.16.11.4781

Proliferative and Inhibitory Activity of Siberian ginseng (Eleutherococcus senticosus) Extract on Cancer Cell Lines; A-549, XWLC-05, HCT-116, CNE and Beas-2b  

Cichello, Simon Angelo (Institute of Nutrition and Food Science, School of Public Health, Kunming Medical University)
Yao, Qian (Yunnan Tumour Hospital)
Dowell, Ashley (Southern Cross Plant Science, Southern Cross University)
Leury, Brian (School of Veterinary and Agricultural Science, The University of Melbourne)
He, Xiao-Qiong (Institute of Nutrition and Food Science, School of Public Health, Kunming Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.16, no.11, 2015 , pp. 4781-4786 More about this Journal
Abstract
Siberian ginseng (Eleutherococcus senticosus) is used primarily as an adaptogen herb and also for its immune stimulant properties in Western herbal medicine. Another closely related species used in East Asian medicine systems i.e. Kampo, TCM (Manchuria, Korea, Japan and Ainu of Hokkaido) and also called Siberian ginseng (Acanthopanax senticosus) also displays immune-stimulant and anti-cancer properties. These may affect tumour growth and also provide an anti-fatigue effect for cancer patients, in particular for those suffering from lung cancer. There is some evidence that a carbohydrate in Siberian ginseng may possess not only immune stimulatory but also anti-tumour effects and also display other various anti-cancer properties. Our study aimed to determine the inhibitory and also proliferative effects of a methanol plant extract of Siberan ginseng (E. senticosus) on various cancer and normal cell lines including: A-549 (small cell lung cancer), XWLC-05 (Yunnan lung cancer cell line), CNE (human nasopharyngeal carcinoma cell line), HCT-116 (human colon cancer) and Beas-2b (human lung epithelial). These cell lines were treated with an extract from E. senticosus that was evaporated and reconstituted in DMSO. Treatment of A-549 (small cell lung cancer) cells with E. senticosus methanolic extract showed a concentration-dependent inhibitory trend from $12.5-50{\mu}g/mL$, and then a plateau, whereas at 12.5 and $25{\mu}g/mL$, there is a slight growth suppression in QBC-939 cells, but then a steady suppression from 50, 100 and $200{\mu}g/mL$. Further, in XWLC-05 (Yunnan lung cancer cell line), E. senticosus methanolic extract displayed an inhibitory effect which plateaued with increasing dosage. Next, in CNE (human nasopharyngeal carcinoma cell line) there was a dose dependent proliferative response, whereas in Beas-2 (human lung epithelial cell line), an inhibitory effect. Finally in colon cancer cell line (HCT-116) we observed an initially weak inhibitory effect and then plateau.
Keywords
Eleutherococcus senticosus; A-549 (small cell lung cancer); XWLC-05 (Yunnan lung cancer cell line);
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Ahn J, Um MY, Lee H, et al (2013). Eleutheroside E, an active component of Eleutherococcus senticosus, ameliorates insulin resistance in type 2 diabetic db/db Mice. Evid Based Complement Alternat Med, 2013, 934183.
2 Asea A, Kaur P, Panossian A, et al (2013). Evaluation of molecular chaperons Hsp72 and neuropeptide Y as characteristic markers of adaptogenic activity of plant extracts. Phytomedicine, 20, 1323-9.   DOI
3 Bespalov VG, Aleksandrov VA, Iaremenko KV, et al (1992). The inhibiting effect of phytoadaptogenic preparations from bioginseng, Eleutherococcus senticosus and Rhaponticum carthamoides on the development of nervous system tumors in rats induced by N-nitrosoethylurea. Vopr Onkol, 38, 1073-80.
4 Bohn B, Nebe CT, Birr C (1987). Flow-cytometric studies with Eleutherococcus senticosus extract as an immunomodulatory agent. Arzneimittelforschung, 37, 1193-6.
5 Bone K (2007). The ultimate herbal compendium. a desktop guide for herbal prescriber. phytotherapy press, Warwick, QLD, Australia.
6 Cicero AF, Derosa G, Brillante R, et al (2004). Effects of siberian ginseng (Eleutherococcus senticosus maxim.) on elderly quality of life: a randomized clinical trial. Arch Gerontol Geriatr Suppl, 9, 69-73.
7 SA Cichello, DP Begg, M Jois, et al (2013). Prevention of diet-induced obesity in C57BL/BJ mice with addition of 2% dietary green tea but not with cocoa or coffee to a high-fat diet. Mediterranean J Nutr Metabolism, 6, 233-8.   DOI
8 Guo S, Liu Y, Lin Z, et al (2014). Effects of eleutheroside B and eleutheroside E on activity of cytochrome P450 in rat liver microsomes. BMC Complement Altern Med, 14, 1.   DOI
9 Ha ES, Hwang SH, Shin KS, et al (2004). Anti-metastatic activity of glycoprotein fractionated from Acanthopanax senticosus, involvement of NK-cell and macrophage activation. Arch Pharm Res, 27, 217-24.   DOI
10 Hacker B, Medon PJ (1984). Cytotoxic effects of Eleutherococcus senticosus aqueous extracts in combination with N6-(delta 2-isopentenyl)-adenosine and 1-beta-D-arabinofuranosylcytosine against L1210 leukemia cells. J Pharm Sci, 73, 270-2.   DOI
11 He XQ, Cichello SA, Duan JL, Zhou J (2014). Canola oil influence on azoxymethane-induced colon carcinogenesis, hypertriglyceridemia and hyperglycemia in Kunming mice. Asian Pac J Cancer Prev, 15, 2477-83.   DOI
12 Herb List (2012). Siberian ginseng retrieved from http://www.herbslist.net/siberian-ginseng-benefits.html
13 Hibasami H, Fujikawa T, Takeda H, et al (2000). Induction of apoptosis by Acanthopanax senticosus HARMS and its component, sesamin in human stomach cancer KATO III cells. Oncol Rep, 7, 1213-6.
14 Kimura Y, Sumiyoshi M (2004). Effects of various Eleutherococcus senticosus cortex on swimming time, natural killer activity and corticosterone level in forced swimming stressed mice. J Ethnopharmacol, 95, 447-53.   DOI
15 Huang DB, Ran RZ, Yu ZF (2005). Effect of Acanthopanax senticosus injection on the activities of human tumor necrosis factor and natural killer cell in blood in the patients with lung cancer. Zhongguo Zhong Yao Za Zhi, 30, 621-4.
16 Huang LZ, Huang BK, Ye Q, et al (2011). Bioactivity-guided fractionation for anti-fatigue property of Acanthopanax senticosus. J Ethnopharmacol, 133, 213-9.   DOI   ScienceOn
17 Ichihara T, Wanibuchi H, Iwai S, et al (2002). White, but not red, ginseng inhibits progression of intestinal carcinogenesis in rats. Asian Pac J Cancer Prev, 3, 243-250.
18 Kuo J, Chen KW, Cheng IS, et al (2010). The effect of eight weeks of supplementation with Eleutherococcus senticosus on endurance capacity and metabolism in human. Chin J Physiol, 53, 105-11.   DOI
19 Lee S, Son D, Ryu J, et al (2004). Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch Pharm Res, 27, 106-10.   DOI
20 Lee D, Park J, Yoon J (2012). Neuroprotective effects of Eleutherococcus senticosus bark on transient global cerebral ischemia in rats. J Ethnopharmacol, 139, 6-11.   DOI
21 Li C, Wang XY, Hu XW, Fang HT, Qiao SY (2008). Determination of eleutheroside B in antifatigue fraction of Acanthopanax senticosus by HPLC. Zhongguo Zhong Yao Za Zhi, 33, 2800-2.
22 Li J, Wei Q, Zuo GW (2014). Ginsenoside Rg1 induces apoptosis through inhibition of the EpoR-mediated JAK2/STAT5 signalling pathway in the TF-1/ Epo human leukemia cell line. Asian Pac J Cancer Prev, 15, 2453-9.   DOI
23 Shan BE, Li QX, Liang WJ (2004). Experimental study on anti-tumor effects of cortex Acanthopanacis senticosus in vivo and in vitro. Zhongguo Zhong Xi Yi Jie He Za Zhi, 24, 55-8.
24 Liu J, Cai SZ, Zhou Y, et al (2012). Senescence as a consequence of ginsenoside rg1 response on k562 human leukemia cell line. Asian Pac J Cancer Prev, 13, 6191-6.   DOI   ScienceOn
25 Sasanami T, Fujishima A, Moriya Y, et al (1982). NK activities in cancer patients-their modification by an immunopotentiator. Gan To Kagaku Ryoho, 9, 2186-92.
26 Schaffler K, Wolf OT, Burkart M (2013). No benefit adding Eleutherococcus senticosus to stress management training in stress-related fatigue/weakness, impaired work or concentration, a randomized controlled study. Pharmacopsychiatry, 46, 181-90.   DOI
27 Soo KH, Young PS, Kyoung KE, et al (2012). Acanthopanax senticosus has a heme oxygenase-1 signaling-dependent effect on Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages. J Ethnopharmacol, 142, 819-28.   DOI
28 Soya H, Deocaris CC, Yamaguchi K, et al (2008). Extract from Acanthopanax senticosus harms (Siberian ginseng) activates NTS and SON/PVN in the rat brain. Biosci Biotechnol Biochem, 72, 2476-80.   DOI
29 Tong L, Huang TY, Li JL (1994). Effects of plant polysaccharides on cell proliferation and cell membrane contents of sialic acid, phospholipid and cholesterol in S 180 and K 562 cells. Zhongguo Zhong Xi Yi Jie He Za Zhi, 14, 482-4.
30 Yamazaki T, Shimosaka S, Sasaki H, et al (2007). (+)-Syringaresinol-di-O-beta-D-glucoside, a phenolic compound from Acanthopanax senticosus harms, suppresses proinflammatory mediators in SW982 human synovial sarcoma cells by inhibiting activating protein-1 and/or nuclear factor-kappaB activities. Toxicol In Vitro, 21, 1530-7.   DOI
31 You ZM, Zhao L, Xia J, et al (2014). Down-regulation of phosphoglucose isomerase/autocrine motility factor enhances gensenoside Rh2 pharmacological action on leukemia $KG1{\alpha}$ cells. Asian Pac J Cancer Prev, 15, 1099-104.   DOI
32 Yoon TJ, Yoo YC, Lee SW, et al (2004). Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action. J Ethnopharmacol, 93, 247-53.   DOI