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http://dx.doi.org/10.7314/APJCP.2014.15.24.10911

Steroidal Saponins from Paris polyphylla Suppress Adhesion, Migration and Invasion of Human Lung Cancer A549 Cells Via Down-Regulating MMP-2 and MMP-9  

He, Hao (School of Pharmaceutical Sciences, Xi'an Medical University)
Zheng, Lei (School of Pharmaceutical Sciences, Xi'an Medical University)
Sun, Yan-Ping (School of Pharmaceutical Sciences, Xi'an Medical University)
Zhang, Guang-Wei (Clinical Medicine College, Xi'an Medical University)
Yue, Zheng-Gang (Shanxi Collaborative Innovation Center of Chinese Medicinal Resource Industrialization, School of Pharmacy, Shanxi College of Traditional Chinese Medicine)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.24, 2015 , pp. 10911-10916 More about this Journal
Abstract
Background: Tumor metastases are the main reasons for oncotherapy failure. Paris polyphylla (Chinese name: Chonglou) has traditionally been used for its anti-cancer actions. In this article, we focus on the regulation of human lung cancer A549 cell metastases and invasion by Paris polyphylla steroidal saponins (PPSS). Materials and Methods: Cell viability was evaluated in A549 cells by MTT assay. Effects of PPSS on invasion and migration were investigated by wound-healing and matrigel invasion chamber assays. Adhesion to type IV collagen and laminin was evaluated by MTT assay. Expression and protease activity of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were analyzed by Western blotting and gelatin zymography, respectively. Results: PPSS exerted growth inhibitory effects on A549 cells, and effectively inhibited A549 cell adhesion, migration and invasion in a concentration-dependent manner. Western blotting and gelatin zymography analysis revealed that PPSS inhibited the expression and secretion of MMP-2 and MMP-9 in A549 cells. Conclusions: PPSS has the potential to suppress the migration, adhesion and invasion of A549 cells. PPSS could be a potential candidate for interventions against lung cancer metastases.
Keywords
Paris polyphylla; steroidal saponins; lung cancer; metastasis; MMP-2; MMP-9;
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Times Cited By KSCI : 6  (Citation Analysis)
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