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http://dx.doi.org/10.7314/APJCP.2014.15.23.10115

IGF-1 from Adipose-Derived Mesenchymal Stem Cells Promotes Radioresistance of Breast Cancer Cells  

Yang, Hui-Ying (Department of Anatomy, Nanfang Hospital, Southern Medical University)
Qu, Rong-Mei (Department of Anatomy, Nanfang Hospital, Southern Medical University)
Lin, Xiao-Shan (Department of Radiation Oncology, Nanfang Hospital, Southern Medical University)
Liu, Tong-Xin (Department of Radiation Oncology, Zhejiang Cancer Hospital)
Sun, Quan-Quan (Department of Radiation Oncology, Zhejiang Cancer Hospital)
Yang, Chun (Department of Anatomy, Nanfang Hospital, Southern Medical University)
Li, Xiao-Hong (Department of Anatomy, Zunyi Medical College)
Lu, Wei (Department of Anatomy, Zunyi Medical College)
Hu, Xiao-Fang (Department of Anatomy, Zunyi Medical College)
Dai, Jing-Xing (Department of Anatomy, Nanfang Hospital, Southern Medical University)
Yuan, Lin (Department of Anatomy, Nanfang Hospital, Southern Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.23, 2015 , pp. 10115-10119 More about this Journal
Abstract
Purpose: The aim of this study was to investigate effects of adipose-derived mesenchymal stem cells (AMSCs) on radioresistance of breast cancer cells. Materials and Methods: MTT assays were used to detect any influence of AMSC supernatants on proliferation of breast cancer cells; cell migration assays were used to determine the effect of breast cancer cells on the recruitment of AMSCs; the cell survival fraction post-irradiation was assessed by clonogenic survival assay; ${\gamma}$-H2AX foci number post-irradiation was determined via fluorescence microscopy; and expression of IGF-1R was detected by Western blotting. Results: AMSC supernatants promoted proliferation and radioresistance of breast cancer cells. Breast cancer cells could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for the radioresistance of breast cancer cells. Conclusions: Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.
Keywords
Adipose-derived mesenchymal stem cells; insulin-like growth factor 1; breast cancer cell proliferation;
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