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http://dx.doi.org/10.7314/APJCP.2014.15.23.10085

Induction of Apoptosis by IGFBP3 Overexpression in Hepatocellular Carcinoma Cells  

Han, Jian-Jun (Department of Cancer Intervention Treatment Center, Shandong Cancer Hospital and Institute)
Xue, De-Wen (Department of Cancer Intervention Treatment Center, Shandong Cancer Hospital and Institute)
Han, Qiu-Rong (Department of Obstetrics and Gynecology, Heze Municipal Hospital)
Liang, Xiao-Hong (Department of Immunology Shandong University School of Medicine)
Xie, Li (Department of Basic Research Center, Shandong Cancer Hospital and Institute)
Li, Sheng (Department of Hepatobiliary Surgery, Shandong Cancer Hospital and Institute)
Wu, Hui-Yong (Department of Cancer Intervention Treatment Center, Shandong Cancer Hospital and Institute)
Song, Bao (Department of Basic Research Center, Shandong Cancer Hospital and Institute)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.23, 2015 , pp. 10085-10089 More about this Journal
Abstract
Background: The insulin-like growth factor (IGF) system comprises a group of proteins that play key roles in regulating cell growth, differentiation, and apoptosis in a variety of cellular systems. The aim of this study was to investigate the role of insulin-like growth factor binding protein 3 (IGFBP3) in hepatocellular carcinoma. Materials and Methods: Expression of IGF2, IGFBP3, and PTEN was analyzed by qRT-PCR. Lentivirus vectors were used to overexpress IGFBP3 in hepatocellular carcinoma cell (HCC) lines. The effect of IGFBP3 on proliferation was investigated by MTT and colony formation assays. Results: Expression of IGF2, IGFBP3, and PTEN in several HCC cell lines was lower than in normal cell lines. After 5-aza-2'-deoxycytidine/trichostatin A treatment, significant demethylation of the promoter region of IGFBP3 was observed in HCC cells. Overexpression of IGFBP3 induced apoptosis and reduced colony formation in HUH7 cells. Conclusions: Expression of IGF2, IGFBP3, and PTEN in several HCC cell lines was lower than in normal cell lines. After 5-aza-2'-deoxycytidine/trichostatin A treatment, significant demethylation of the promoter region of IGFBP3 was observed in HCC cells. Overexpression of IGFBP3 induced apoptosis and reduced colony formation in HUH7 cells.
Keywords
Hepatocellular carcinoma; IGF signaling pathway; IGFBP3; proliferation;
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