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http://dx.doi.org/10.7314/APJCP.2014.15.9.4079

Increased Argonaute 2 Expression in Gliomas and its Association with Tumor Progression and Poor Prognosis  

Feng, Bo (Department of Neurology, Hospital Affiliated to Binzhou medical University)
Hu, Peng (Department of Spine, Hospital Affiliated to Binzhou medical University)
Lu, Shu-Jun (Department of Neurology, Hospital Affiliated to Binzhou medical University)
Chen, Jin-Bo (Department of Neurology, Hospital Affiliated to Binzhou medical University)
Ge, Ru-Li (Department of Neurology, Hospital Affiliated to Binzhou medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.9, 2014 , pp. 4079-4083 More about this Journal
Abstract
Background: Previous studies have showed that argonaute 2 is a potential factor related to genesis of several cancers, however, there have been no reports concerning gliomas. Methods: Paraffin specimens of 129 brain glioma cases were collected from a hospital affiliated to Binzhou Medical University from January 2008 to July 2013. We examined both argonaute 2 mRNA and protein expression by real-time quantitative PCR (qRT-PCR), Western blot analysis, and immunohistochemistry (IHC). The survival curves of the patients were determined using the Kaplan-Meier method and Cox regression, and the log-rank test was used for statistical evaluations. Results: Both argonaute 2 mRNA and protein were upregulated in high-grade when compared to low-grade tumor tissues. Multivariate analysis revealed that argonaute 2 protein expression was independently associated with the overall survival (HR=4.587, 95% CI: 3.001-6.993; P=0.002), and that argonaute 2 protein expression and WHO grading were independent prognostic factors for progression-free survival (HR=4.792, 95% CI: 3.993-5.672; P<0.001, and HR=2.109, 95% CI: 1.278-8.229; P=0.039, respectively). Kaplan-Meier analysis with the log-rank test indicated that high argonaute 2 protein expression had a significant impact on overall survival (P=0.0169) and progression-free survival (P=0.0324). Conclusions: The present study showed that argonaute 2 expression is up-regulated in gliomas. Argonaute 2 might also serve as a novel prognostic marker.
Keywords
Glioma; argonaute 2; prognostic marker;
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