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http://dx.doi.org/10.7314/APJCP.2014.15.9.4013

Low Expression of the FoxO4 Gene may Contribute to the Phenomenon of EMT in Non-small Cell Lung Cancer  

Xu, Ming-Ming (Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University)
Mao, Guo-Xin (Department of Oncology, Affiliated Hospital of Nantong University)
Liu, Jian (Department of Oncology, Affiliated Hospital of Nantong University)
Li, Jian-Chao (Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University)
Huang, Hua (Department of Pathology, Affiliated Hospital of Nantong University)
Liu, Yi-Fei (Department of Pathology, Affiliated Hospital of Nantong University)
Liu, Jun-Hua (Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.9, 2014 , pp. 4013-4018 More about this Journal
Abstract
Because of its importance in tumor invasion and metastasis, the epithelial-mesenchymal transition (EMT) has become a research focus in the field of cancer. Recently, evidence has been presented that FoxO4 might be involved in EMT. Our study aimed to detect the expression of FoxO4, E-cadherin and vimentin in non-small cell lung cancers (NSCLCs). We also investigated clinical features and their correlations with the markers. In our study, FoxO4, E-cadherin and vimentin were assessed by immunohistochemistry in a tissue microarray (TMA) containing 150 cases of NSCLC. In addition, the expression level of FoxO4 protein was determined by Western blotting. The percentages of FoxO4, E-cadherin and vimentin positive expression in NSCLCs were 42.7%, 38.7% and 55.3%, respectively. Immunoreactivity of FoxO4 was low in NSCLC when compared with paired normal lung tissues. There were significant correlations between FoxO4 and TNM stage (P<0.001), histological differentiation (P=0.004) and lymph node metastasis (P<0.001), but no significant links with age (P=0.323), gender (P=0.410), tumor size (P=0.084), smoking status (P=0.721) and histological type (P=0.281). Our study showed that low expression of FoxO4 correlated with decreased expression of E-cadherin and elevated expression of vimentin. Cox regression analysis indicated FoxO4 to be an independent prognostic factor in NSCLC (P=0.046). These data suggested that FoxO4 might inhibit the process of EMT in NSCLC, and might therefore be a target for therapy.
Keywords
FoxO4; E-cadherin; vimentin; epithelial-mesenchymal transition; NSCLC;
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