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http://dx.doi.org/10.7314/APJCP.2014.15.9.3987

Curcumin Induces Apoptosis in SGC-7901 Gastric Adenocarcinoma Cells via Regulation of Mitochondrial Signaling Pathways  

Xue, Xia (Department of Pharmacy, The Second Hospital of Shandong University)
Yu, Jin-Long (Department of Pharmacy, The Second Hospital of Shandong University)
Sun, De-Qing (Department of Pharmacy, The Second Hospital of Shandong University)
Kong, Feng (Central Laboratory, The Second Hospital of Shandong University)
Qu, Xian-Jun (School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University)
Zou, Wen (Department of Pharmacy, The Second Hospital of Shandong University)
Wu, Jing (Department of Pharmacy, The Second Hospital of Shandong University)
Wang, Rong-Mei (Department of Pharmacy, The Second Hospital of Shandong University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.9, 2014 , pp. 3987-3992 More about this Journal
Abstract
Curcumin, a polyphenol compound derived from the rhizome of the plant Curcuma longa L. has been verified as an anticancer compound against several types of cancer. However, understanding of the molecular mechanisms by which it induces apoptosis is limited. In this study, the anticancer efficacy of curcumin was investigated in human gastric adenocarcinoma SGC-7901 cells. The results demonstrated that curcumin induced morphological changes and decreased cell viability. Apoptosis triggered by curcumin was visualized using Annexin V-FITC/7-AAD staining. Curcumin-induced apoptosis of SGC-7901 cells was associated with the dissipation of mitochondrial membrane potential (MMP) and the release of cytochrome c into the cytosol. Furthermore, the down-regulation of Bcl-2 and up-regulation of Bax that led to the cleavage of caspase-3 and increased cleaved PARP was observed in SGC-7901 cells treated with curcumin. Therefore, curcumin-induced apoptosis of SGC-7901 cells might be mediated through the mitochondria pathway, which gives the rationale for in vivo studies on the utilization of curcumin as a potential cancer therapeutic compound.
Keywords
Curcumin; human gastric adenocarcinoma; mitochondrial signaling pathway; apoptosis;
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1 Wang YQ, Zhang SJ, Lu H, et al (2013). A C 21-Steroidal Glycoside Isolated from the Roots of Cynanchum auriculatum Induces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells. Evid Based Complement Alternat Med, 2013, 180839.
2 Zhang S, Kuang G, Zhao G, et al (2013). Involvement of the mitochondrial p53 pathway in PBDE-47-induced SH-SY5Y cells apoptosis and its underlying activation mechanism. Food Chem Toxicol, 62, 699-706.   DOI   ScienceOn
3 Szaflarski W, Sujka-Kordowska P, Januchowski R, et al (2013). Nuclear localization of P-glycoprotein is responsible for protection of the nucleus from doxorubicin in the resistant LoVo cell line. Biomed Pharmacother, 67, 497-502.   DOI   ScienceOn
4 Tomek M, Akiyama T, Dass CR (2012). Role of Bcl-2 in tumour cell survival and implications for pharmacotherapy. J Pharm Pharmacol, 64, 1695-702.   DOI   ScienceOn
5 Tyagi M, Bhattacharyya R, Bauri AK, et al (2014). DNA damage dependent activation of checkpoint kinase-1 and mitogen-activated protein kinase-p38 are required in malabaricone C-induced mitochondrial cell death. Biochim Biophys Acta, 1840, 1014-27.   DOI   ScienceOn
6 Wang RF, Zhang LH, Shan LH, et al (2013). Effects of the fibroblast activation protein on the invasion and migration of gastric cancer. Exp Mol Pathol, 95, 350-56.   DOI   ScienceOn
7 Wong RS (2011). Apoptosis in cancer: from pathogenesis to treatment. J Exp Clin Cancer Res, 30, 87.   DOI
8 Xue X, Sun DF, Sun CC, et al (2012). Inhibitory effect of riccardin D on growth of human non-small cell lung cancer: in vitro and in vivo studies. Lung Cancer, 76, 300-8.   DOI   ScienceOn
9 Zhang C, Yuan XR, Li HY, et al (2014). Downregualtion of dynamin-related protein 1 attenuates glutamate-induced excitotoxicity via regulating mitochondrial function in a calcium dependent manner in HT22 cells. Biochem Biophys Res Commun, 443, 138-43.   DOI   ScienceOn
10 Zhang GH, Cai LJ, Wang YF, et al (2013). Novel compound PS-101 exhibits selective inhibition in non-small-cell lung cancer cell by blocking the EGFR-driven antiapoptotic pathway. Biochem Pharmacol, 86, 1721-30.   DOI   ScienceOn
11 Mendez J, Morales Cruz M, Delgado Y, et al (2014). Delivery of chemically glycosylated cytochrome c immobilized in mesoporous silica nanoparticles induces apoptosis in hela cancer cells. Mol Pharm, 11, 102-11.   DOI   ScienceOn
12 Lorenzen S, Pauligk C, Homann N, et al(2013). Feasibility of perioperative chemotherapy with infusional 5-FU, leucovorin, and oxaliplatin with (FLOT) or without (FLO) docetaxel in elderly patients with locally advanced esophagogastric cancer. Br J Cancer, 108, 519-26.   DOI   ScienceOn
13 Lucena FR, de Araujo LC, Rodrigues Mdo D, et al (2013). Induction of cancer cell death by apoptosis and slow release of 5-fluoracil from metal-organic frameworks Cu-BTC. Biomed Pharmacother, 67, 707-13.   DOI   ScienceOn
14 Ma JQ, Ding J, Zhang L, et al (2014). Hepatoprotective properties of sesamin against CCl4 induced oxidative stress-mediated apoptosis in mice via JNK pathway. Food Chem Toxicol, 64, 41-8.   DOI   ScienceOn
15 Singh DV, Agarwal S, Singh P, Godbole MM, Misra K (2013). Curcumin conjugates induce apoptosis via a mitochondrion dependent pathway in MCF-7 and MDA-MB-231 cell lines. Asian Pac J Cancer Prev, 14, 5797-804.   과학기술학회마을   DOI   ScienceOn
16 Sharoar MG, Islam MI, Shahnawaz M, et al (2014). Amyloid $\beta$ binds procaspase-9 to inhibit assembly of Apaf-1 apoptosome and intrinsic apoptosis pathway. Biochim Biophys Acta, 1843, 685-93.   DOI   ScienceOn
17 Shin NR, Jeong EH, Choi CI, et al (2012). Overexpression of Snail is associated with lymph node metastasis and poor prognosis in patients with gastric cancer. BMC Cancer, 12, 521.   DOI   ScienceOn
18 Shoji M, Nakagawa K, Watanabe A, et al (2014). Comparison of the effects of curcumin and curcumin glucuronide in human hepatocellular carcinoma HepG2 cells. Food Chem, 151, 126-32.   DOI   ScienceOn
19 Ko JK, Choi KH, Peng J, et al (2011). Amphipathic tail-anchoring peptide and Bcl-2 homology domain-3 (BH3) peptides from Bcl-2 family proteins induce apoptosis through different mechanisms. J Biol Chem, 286, 9038-48.   DOI   ScienceOn
20 Hossein G1, Keshavarz M, Ahmadi S, et al (2013). Synergistic effects of pectasol-C modified citrus pectin an inhibitor of galectin-3 and paclitaxel on apoptosis of human SKOV-3 ovarian cancer cells. Asian Pac J Cancer Prev, 14, 7561-8.   과학기술학회마을   DOI   ScienceOn
21 Huang JX, Zhao K, Lin M, et al (2013). HER2 gene amplification in esophageal squamous cell carcinoma is less than in gastroesophageal junction and gastric adenocarcinoma. Oncol Lett, 6, 13-8.   DOI
22 Li Y, Zhang S, Geng JX, Hu XY (2013). Curcumin inhibits human non-small cell lung cancer A549 cell proliferation through regulation of Bcl-2/Bax and cytochrome C. Asian Pac J Cancer Prev, 14, 4599-602.   과학기술학회마을   DOI   ScienceOn
23 Jiang AP, Zhou DH, Meng XL, et al (2014). Down-regulation of epidermal growth factor receptor by curcumin-induced UBE1L in human bronchial epithelial cells. J Nutr Biochem, 25, 241-9.   DOI   ScienceOn
24 Li L, Yang F, Wang X, et al (2014). Effect of 15-hydroxyprostaglandin dehydrogenase gene on the proliferation of gastric cancer cell murine forestomach carcinoma. Exp Ther Med, 7, 290-4.   DOI
25 Li PM, Li YL, Liu B, et al (2014). Curcumin inhibits MHCC97H liver cancer cells by activating ROS/TLR-4/caspase signaling pathway. Asian Pac J Cancer Prev, 15, 2329-34.   과학기술학회마을   DOI   ScienceOn
26 Liu Z, Lu H, Jiang Z, et al (2005). Apolipoprotein l6, a novel proapoptotic Bcl-2 homology 3-only protein, induces mitochondria-mediated apoptosis in cancer cells. Mol Cancer Res, 3, 21-31.
27 Chan SL, Yu VC (2004). Proteins of the bcl-2 family in apoptosis signalling: from mechanistic insights to therapeutic opportunities. Clin Exp Pharmacol Physiol, 31, 119-28.   DOI   ScienceOn
28 Ajani JA, Buyse M, Lichinitser M, et al (2013). Combination of cisplatin/S-1 in the treatment of patients with advanced gastric or gastroesophageal adenocarcinoma: Results of noninferiority and safety analyses compared with cisplatin/5-fluorouracil in the First-Line Advanced Gastric Cancer Study. Eur J Cancer, 49, 3616-24.   DOI   ScienceOn
29 Banjerdpongchai R, Khaw-On P (2013). Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells. Asian Pac J Cancer Prev, 14, 7537-42.   과학기술학회마을   DOI   ScienceOn
30 Aporta A, Catalan E, Galan-Malo P, et al (2014). Granulysin induces apoptotic cell death and cleavage of the autophagy regulator Atg5 in human hematological tumors. Biochem Pharmacol, 87, 410-23.   DOI   ScienceOn
31 Chen SS, Yang XC, Chi F, et al (2013). A phase II study of preoperative chemotherapy with modified FOLFOX6 followed by surgery and postoperative chemoradiation in patients with localized gastric adenocarcinoma. Oncol Res, 20, 327-32.   DOI
32 Gajek A, Denel M, Bukowska B, et al (2014). Pro-apoptotic activity of new analog of anthracyclines-WP 631 in advanced ovarian cancer cell line. Toxicol In vitro, 28, 273-81.   DOI   ScienceOn
33 Gopal PK, Paul M, Paul S (2014). Curcumin induces caspase mediated apoptosis in JURKAT cells by disrupting the redox balance. Asian Pac J Cancer Prev, 15, 93-100.   과학기술학회마을   DOI   ScienceOn
34 Hasan M, Belhaj N, Benachour H, et al (2014). Liposome encapsulation of curcumin: Physico-chemical characterizations and effects on MCF7 cancer cell proliferation. Int J Pharm, 461, 519-28.   DOI   ScienceOn
35 Liu B, Xu N, Man Y, et al (2013). Apoptosis in living animals is assisted by scavenger cells and thus may not mainly go through the cytochrome c-caspase pathway. J Cancer, 4, 716-23.   DOI   ScienceOn
36 Rodriguez-Nieto S, Zhivotovsky B (2006). Role of alterations in the apoptotic machinery in sensitivity of cancer cells to treatment. Curr Pharm Des, 12, 4411-25.   DOI   ScienceOn