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http://dx.doi.org/10.7314/APJCP.2014.15.7.3293

Inhibitory Effects of α-Pinene on Hepatoma Carcinoma Cell Proliferation  

Chen, Wei-Qiang (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University)
Xu, Bin (School of Life Science and Biopharmaceutical Sciences, Guangdong Pharmaceutical University)
Mao, Jian-Wen (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University)
Wei, Feng-Xiang (The Genetics Laboratory, Maternity and Child Healthcare Hospital, Longgang District)
Li, Ming (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University)
Liu, Tao (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University)
Jin, Xiao-Bao (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University)
Zhang, Li-Rong (School of Basic Medicine and Guangdong Key Laboratory for Bioactive Drug Research, Guangdong Pharmaceutical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.7, 2014 , pp. 3293-3297 More about this Journal
Abstract
Background: Pine needle oil from crude extract of pine needles has anti-tumor effects, but the effective component is not known. Methods: In the present study, compounds from a steam distillation extract of pine needles were isolated and characterized. Alpha-pinene was identified as an active anti-proliferative compound on hepatoma carcinoma BEL-7402 cells using the MTT assay. Results: Further experiments showed that ${\alpha}$-pinene inhibited BEL-7402 cells by arresting cell growth in the G2/M phase of the cell cycle, downregulating Cdc25C mRNA and protein expression, and reducing cycle dependence on kinase 1(CDK1) activity. Conclusion: Taken together, these findings indicate that ${\alpha}$-pinene may be useful as a potential anti-tumor drug.
Keywords
${\alpha}$-pinene; hepatoma carcinoma; proliferation; cell cycle proteins;
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