Browse > Article
http://dx.doi.org/10.7314/APJCP.2014.15.7.3051

Expression and Effects of JMJD2A Histone Demethylase in Endometrial Carcinoma  

Wang, Hong-Li (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Liu, Mei-Mei (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Ma, Xin (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Fang, Lei (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Zhang, Zong-Feng (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Song, Tie-Fang (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Gao, Jia-Yin (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Kuang, Ye (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Jiang, Jing (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Li, Lin (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Wang, Yang-Yang (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Li, Pei-Ling (Department of Obstetrics and Gynaecology, Second Affiliated Hospital of Harbin Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.7, 2014 , pp. 3051-3056 More about this Journal
Abstract
Previous studies have demonstrated that JMJD2A is a potential oncogene and is overexpressed in human tumors. However, its role in the endometrial carcinoma remains largely unknown. In this study, we discovered that JMJD2A was overexpressed in endometrial carcinoma, using immunohistochemistry, quantitative realtime polymerase chain reaction, and western blotting. Downregulation of JMJD2A led to reduced endometrial carcinoma RL95-2 and ISK cell proliferation, invasion and metastasis as asessed with cell counting kit-8, cell migration and invasive assays. Collectively, our results support that JMJD2A is a promoter of endometrial carcinoma cell proliferation and survival, and is a potential novel drug target.
Keywords
JMJD2A; endometrial carcinoma; cell proliferation; tumor migration; tumor invasion;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Salvesen HB, Haldorsen IS, Trovik J (2012). Markers for individualised therapy in endometrial carcinoma. Lancet Oncol, 13, 353-61.   DOI   ScienceOn
2 Tsukada Y, Fang J, Erdjument-Bromage H, et al (2006). Histone demethylation by a family of JmjC domain-containing proteins. Nature, 439, 811-6.   DOI
3 Vousden KH, and Prives C (2009). Blinded by the light: the growing complexity of p53. Cell, 137, 413-31.   DOI   ScienceOn
4 Wang XW, Chen YL, Luo YL, Liu QY (2011). No association between the CYP1B1 C4326G polymorphism and endometrial cancer risk: a meta-analysis. Asian Pac J Cancer Prev, 12, 2343-8.
5 Qin C, Nguyen T, Stewart J, et al (2002). Estrogen up-regulation of p53 gene expression in MCF-7 breast cancer cells is mediated by calmodulin kinase IV-dependent activation of anuclearfactorB/CCAAT- bindingtranscriptionfactor-1complex. Mol Endocrinol, 16, 1793-809.   DOI   ScienceOn
6 Ryan AJ, Susil B, Jobling TW, Oehler MK (2005). Endometrial cancer. Cell and Tissue Research, 322, 53-61.   DOI
7 Shah YM, Basrur V, Rowan BG (2004). Selective estrogen receptor modulator regulated proteins in endometrial cancer cells. Mol Cell Endocrinol, 219, 127-39.   DOI   ScienceOn
8 Shin S, Janknecht R (2007). Activation of androgen receptor by histone demethylases JMJD2A and JMJD2D. Biochem Biophys Res Commun, 359, 742-6.   DOI   ScienceOn
9 Shirley SH, Rundhaug JE, Tian J, et al (2009). Transcriptional regulation of estrogen receptor by p53 in human breast cancer cells. Cancer Res, 69, 3405-14.   DOI   ScienceOn
10 Siegel R, Naishadham D, Jemal A (2013). Cancer statistics. CA Cancer J Clin, 63, 11-30.   DOI   ScienceOn
11 Sirbasku DA, Moreno-Cuevas JE (2000). Estrogen mitogenic action. ii. negative regulation of the steroid hormone-responsive growth of cell lines derived from human and rodent target tissue tumors and conceptual implications. In Vitro Cell Dev Biol Anim, 36, 428-46.   DOI
12 Soto AM, Sonnenschein C (1987). Cell proliferation of estrogen-sensitive cells: the case for negative control. Endocr Rev, 8, 44-52.   DOI   ScienceOn
13 Thanapprapasr D, Thanapprapasr K (2013). Molecular therapy as a future strategy in endometrial cancer. Asian Pac J Cancer Prev, 14, 3419-23.   과학기술학회마을   DOI   ScienceOn
14 Thomas DB (1984). Do hormones cause breast cancer? Cancer, 53, 595-604.   DOI
15 Cloos PA, Christensen J, Agger K, et al (2006). The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3. Nature, 442, 307-11.   DOI   ScienceOn
16 Di Cristofano A, Ellenson LH (2007). Endometrial carcinoma. Ann Rev Pathol, 2, 57-85.   DOI   ScienceOn
17 Berstein LM, Tchernobrovkina AE, Gamajunova VB, et al (2003). Tumor estrogen content and clinico-morphological and endocrine features of endometrial cancer. J Cancer Res Clin Oncol, 129, 245-9.
18 Chao A, Wang TH, Lee YS, et al (2006). Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression. Int J Cancer, 119, 91-8.   DOI   ScienceOn
19 Dickson RB, Lippman ME (1995). Growth factors in breast cancer. Endocr Rev, 16, 559-89.   DOI
20 Hecht JL, Mutter GL (2006). Molecular and pathologic aspects ofendometrial carcinogenesis. J Clin Oncol, 24, 4783-91.   DOI   ScienceOn
21 Katoh M, Katoh M (2004). Identification and characterization of JMJD2 family genes in silico. Int J Oncol, 24, 1623-8.
22 Angeloni SV, Martin MB, Garcia-Morales P, Castro-Galache MD, et al (2004). Regulation of estrogen receptor-alpha expression by the tumor suppressor gene p53 in MCF-7 cells. J Endocrinol, 180, 497-504.   DOI   ScienceOn
23 Kauffman EC, Robinson BD, Downes MJ, et al (2011). Role of androgen receptor and associated lysine-demethylase coregulators, LSD1 and JMJD2A, in localized and advanced human bladder cancer. Mol Carcinog, 50, 931-44.   DOI   ScienceOn
24 Kim TD, Shin S, Berry WL, et al (2012). The JMJD2A demethylase regulates apoptosis and proliferation in colon cancer cells. J Cell Biochem, 113, 1368-76.   DOI   ScienceOn
25 Amant F, Moerman P, Neven P, et al (2005). Endometrial cancer. Lancet, 366, 491-505.   DOI   ScienceOn
26 Arafa M, Somja J, Dehan P, Kridelka F, Goffin F, et al (2010). Current concepts in the pathology and epigenetics of endometrial carcinoma. Pathology, 42, 613-7.   DOI   ScienceOn
27 Berger CE, Qian Y, Liu G, Chen H, Chen X (2012). p53, a target of estrogen receptor (ER)${\alpha}$, modulates DNA damage-induced growth suppression in ER-positivebreast cancer cells. J Biol Chem, 287, 30117-27.   DOI
28 Berry WL, Shin S, Lightfoot SA, Janknecht R (2012). Oncogenic features of the JMJD2A histone demethylase in breast cancer. Int J Oncol, 41, 1701-6.   DOI
29 Li BX, Zhang MC, Luo CL, et al (2011). Effects of RNA interference-mediated gene silencing of JMJD2A on human breast cancer cell line MDA-MB-231 in vitro. J Exp Clin Cancer Res, 30, 90.   DOI