Browse > Article
http://dx.doi.org/10.7314/APJCP.2014.15.7.2993

Extract of Saccharina japonica Induces Apoptosis companied by Cell Cycle Arrest and Endoplasmic Reticulum Stress in SK-Hep1 Human Hepatocellular Carcinoma Cells  

Jung, Hyun Il (Department of Microbiology, College of Natural Sciences, Pukyong National University)
Jo, Mi Jeong (Department of Microbiology, College of Natural Sciences, Pukyong National University)
Kim, Hyung-Rak (Department of Food Science and Nutrition, College of Fishery Sciences, Pukyong National University)
Choi, Yung Hyun (Department of Biochemistry, College of Oriental Medicine, Dongeui University)
Kim, Gun-Do (Department of Microbiology, College of Natural Sciences, Pukyong National University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.7, 2014 , pp. 2993-2999 More about this Journal
Abstract
Saccharina japonica is a family member of Phaeophyceae (brown macro-alga) and extensively cultivated in China, Japan and Korea. Here, the potential anti-cancer effect of n-hexane fraction of S. japonica was evaluated in SK-Hep1 human hepatocellular carcinoma cells. The N-hexane fraction reduced cell viability and increased the numbers of apoptotic cells in a both dose- and time-dependent manner. Apoptosis was activated by both caspase-dependent and independent pathways. The caspase-dependent cell death pathway is mediated by cell surface death receptors and activated caspase-8 amplified the apoptotic signal either through direct activation of downstream caspase-3 or pro-apoptotic proteins (Bad, Bax and Bak) subsequently leading to the release of cytochrome c. On the other hand, caspase-independent apoptosis appeared mediated by disruption of mitochondrial membrane potential and translocation of AIF to the nucleus where they induced chromatin condensation and/or large-scale DNA fragmentation. In addition, the n-hexane fraction induced endoplasmic reticulum (ER)-stress and cell cycle arrest. The results suggested that potential anti-cancer effects of n-hexane extract from S. japonica on SK-Hep1 cells.
Keywords
Caspase-dependent/independent apoptosis; Hep1 cells; cell cycle arrest; ER-stress; Saccharina japonica;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Plouguerne E, de Souza LM, Sassaki GL,et al (2013). Antiviral Sulfoquinovosyldiacylglycerols (SQDGs) from the Brazilian brown seaweed Sargassum vulgare. Mar Drugs, 11, 4628-40.   DOI
2 Senthilkumar K, Manivasagan P, Venkatesan J, et al (2013). Brown seaweed fucoidan: biological activity and apoptosis, growth signaling mechanism in cancer. Int J Biol Macromol, 60, 366-74.   DOI
3 Sevrioukova IF (2011). Apoptosis-inducing factor: structure, function, and redox regulation. Antioxid Redox Signal, 14, 2545-79.   DOI
4 Voeltz GK, Rolls MM, Rapoport TA (2002). Structural organization of the endoplasmic reticulum. EMBO Rep, 3, 944-50.   DOI
5 Wang WA, Groenendyk J, Michalak M (2014). Endoplasmic reticulum stress associated responses in cancer. Biochim Biophys Acta, [Epub ahead of print].
6 Wurstle ML, Laussmann MA, Rehm M (2012). The central role of initiator caspase-9 in apoptosis signal transduction and the regulation of its activation and activity on the apoptosome. Exp Cell Res, 318, 1213-20.   DOI
7 Zhao YJ, Wang JH, Fu B, et al (2009). Effects of 3-aminobenzamide on expressions of poly (ADP ribose) polymerase and apoptosis inducing factor in cardiomyocytes of rats with acute myocardial infarction. Chin Med J, 122, 1322-7.
8 Kaufman RJ (1999). Stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. Genes Dev, 13, 1211-3.   DOI   ScienceOn
9 Jacobson MD, Weil M, Raff MC (1997). Programmed cell death in animal development. Cell, 88, 347-54.   DOI   ScienceOn
10 Jin Z, El-Deiry WS (2005). Overview of cell death signaling pathways. Cancer Biol Ther, 4, 139-63.
11 Jo MJ, Kim HR, Kim G-D (2012). The anticancer effects of Saccharina japonica on 267B1/K-ras human prostate cancer cells. Int J Oncol, 41, 1789-97.   DOI
12 Khan KH, Blanco-Codesido M, Molife LR (2013). Cancer therapeutics: Targeting the apoptotic pathway. Crit Rev Oncol Hematol, 13, 267-9.
13 Kim J, Jayaprakasha GK, Vikram A, et al (2012). Methyl nomilinate from citrus can modulate cell cycle regulators to induce cytotoxicity in human colon cancer (SW480) cells in vitro. Toxicol in vitro, 26, 1216-23.   DOI
14 Li XC, Jacob MR, Ding Y, et al (2006). Capisterones A and B, which enhance fluconazole activity in Saccharomyces cerevisiae, from the marine green alga Penicillus capitatus. J Nat Prod, 69, 542-6.   DOI
15 Manivannan K, Karthikai Devi G, Anantharaman P, et al (2011). Antimicrobial potential of selected brown seaweeds from Vedalai coastal waters, Gulf of Mannar. Asian Pac J Trop Biomed, 1, 114-20.   DOI
16 Matsuhiro B, Conte AF, Damonte EB, et al (2005). Structural analysis and antiviral activity of a sulfated galactan from the red seaweed Schizymenia binderi (Gigartinales, Rhodophyta). Carbohydr Res, 340, 2392-402.   DOI
17 Muthuirulappan S, Francis SP (2013). Anti-cancer mechanism and possibility of nano-suspension formulations for a marine algae product fucoxanthin. Asian Pac J Cancer Prev, 14, 2213-6.   DOI   ScienceOn
18 Boland ML, Chourasia AH, Macleod KF (2013). Mitochondrial dysfunction in Cancer. Front Oncol, 3, 292.
19 Ahn HK, Lee S, Sun JM, et al (2011). Sequential therapy with sunitinib and sorafenib in metastatic hepatocellular carcinoma. Invest New Drugs, 30, 1768-72.
20 Allan LA, Clarke PR (2009). Apoptosis and autophagy: Regulation of caspase-9 by phosphorylation. FEBS J, 276, 6063-73.   DOI   ScienceOn
21 Ashkenazi A, Dixit VM (1998). Death receptors: signaling and modulation. Science, 281, 1305-8.   DOI   ScienceOn
22 Bossy-Wetzel E, Newmeyer DD, Green DR (1998). Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization. EMBO J, 17, 37-49.   DOI   ScienceOn
23 Cregan SP, Dawson VL, Slack RS (2004). Role of AIF in caspase-dependent and caspase-independent cell death. Oncogene, 23, 2785-96.   DOI   ScienceOn
24 Donnelly N, Gorman AM, Gupta S, et al (2013). The $eIF2{\alpha}$ kinases: their structures and functions. Cell Mol Life Sci, 70, 3493-11.   DOI
25 Fitton JH (2003). Brown marine algae: a survey of therapeutic potentials. Altern Complement Ther, 9, 29-33.   DOI
26 Funahashi H, Imai T, Tanaka Y, et al (1999). Wakame seaweed suppresses the proliferation of 7, 12-dimethylbenz (a)-anthracene-induced mammary tumors in rats. Cancer Sci, 90, 922-7.   DOI
27 Hu Q, Wu D, Chen W, et al (2013). Proteolytic processing of the caspase-9 zymogen is required for apoptosome-mediated activation of caspase-9. J Biol Chem, 288, 15142-7.   DOI
28 Itoh H, Noda H, Amano H, et al (1995). Immunological analysis of inhibition of lung metastases by fucoidan (GIV-A) prepared from brown seaweed Sargassum thunbergii. Anticancer Res, 15, 1937-47.
29 Oyadomari S, Araki E, Mori M (2002). Endoplasmic reticulum stress-mediated apoptosis in pancreatic ${\beta}$-cells. Apoptosis, 7, 335-45.   DOI   ScienceOn
30 Rebecca S, Deepa N, Ahmedin J (2013). Cancer statistics, 2013. CA Cancer J Clin, 63, 11-30.   DOI   ScienceOn
31 Ohigashi H, Sakai Y, Yamaguchi K, et al (1992). Possible anti-tumor promoting properties of marine algae and in vivo activity of Wakame seaweed extract. Biosci Biotech Biochem, 56, 994-5.   DOI   ScienceOn