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http://dx.doi.org/10.7314/APJCP.2014.15.6.2655

Talin-1 Correlates with Reduced Invasion and Migration in Human Hepatocellular Carcinoma Cells  

Fang, Kun-Peng (First Affiliated Hospital of Anhui Medical University)
Zhang, Jian-Lin (First Affiliated Hospital of Anhui Medical University)
Ren, Yan-Hong (First Affiliated Hospital of Anhui Medical University)
Qian, Ye-Ben (First Affiliated Hospital of Anhui Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.6, 2014 , pp. 2655-2661 More about this Journal
Abstract
Background: Talin-1 is a cytoskeleton protein that participates in cell migration and plays a role in tumor formation, migration, and metastasis in different types of cancer. Chinese investigators have observed that the levels of Talin-1 protein and mRNA expression in HCC tissues are significantly lower than in the adjacent non-cancerous tissue. However, Japanese investigators have reported that Talin-1 is upregulated in HCC. Tln2 as homologous gene of Tln-1, which encodes a very similar protein, but the role of Talin-2 is very little known in primary liver cancer (PLC). We investigated whether the expression of Talin-1 in PLC may be associated with the histological subtype as well as the role of Talin-1 in tumor cell invasion and migration using human hepatocellular carcinoma cell lines. Materials and Methods: We measured the mRNA expression levels of Talin-1 and Talin-2 in five human liver cancer cell lines and normal human liver cell ($LO_2$ cell line) by real-time PCR and the protein expression levels of Talin-1 by Western blot. Migration and invasion of the cells were assessed using transwell assays and cell scratch experiments, respectively, and proliferation was assessed by soft AGAR colony formation. Results: Talin-1 and Talin-2 expression differed significantly between the five human liver cancer cell lines and $LO_2$ cell line (p<0.05). Compared with the $LO_2$ cell line, the invasion and migration capabilities of the five cancer cell lines differed significantly (p<0.05). Similarly, the colony-forming ability differed (p<0.05). Conclusions: High levels of Talin-1 expression are correlated with reduced invasion and migration as well as decreased malignancy in human liver cancer cell lines; the suppression of Talin-1 promotes invasion and migration. In addition, Talin-2 may be correlated with invasion and migration in human hepatocellular carcinoma.
Keywords
Hepatocellular carcinoma; HCC; Talin-1; Talin-2; migration; invasion; cell lines;
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