Browse > Article
http://dx.doi.org/10.7314/APJCP.2014.15.5.2319

GSTT1 is Deregulated in Left Colon Tumors  

Coskunpinar, Ender (Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University)
Canbay, Emel (Kocaeli-Derince Education and Research Hospital)
Oltulu, Yasemin Musteri (Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University)
Tiryakioglu, Necip Ozan (Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University)
Bugra, Dursun (American Hospital, General Surgery Clinic)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.5, 2014 , pp. 2319-2321 More about this Journal
Abstract
Our aim was to determine GSTT1 expression levels in left colon tumors and paired normal tissue in order to identify specific alterations in GSTT1 mRNA levels. Alterations in GSTT1 expression in twenty-four left-sided colon tumors and paired cancer free tissue were determined by qRT-PCR. Significant fold changes were determined with t-test. When compared with cancer free tissue, left colon cancers showed a significant decrease in GSTT1 expression. However, GSTT1 mRNA levels among different grades increased gradually in correlation with tumor grade. Our results suggest that downregulation of GSTT1 in left-sided colon cancers is an early event and is reversed with cancer progression, probably due to cellular defense mechanisms as a response to changes in the microenvironment.
Keywords
Deregulation; GSTT1 expression; left colon cancer;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Mannervik, B (2012). Five decades with glutathione and the GSTome. J Biol Chem, 287, 6072-83.   DOI
2 Xu D, Yan S, Yin J, Zhang PN (2011). Null genotype of GSTT1 contributes to colorectal cancer risk in Asian populations: evidence from a meta-analysis. Asian Pac J Cancer Prev, 12, 2279-84.
3 Yi SM, Li G-Y (2012). Null genotype of GSTT1 contributes to esophageal cancer risk in Asian populations: evidence from a meta-analysis Asian Pac J Cancer Prev, 13, 4967-71.   과학기술학회마을   DOI   ScienceOn
4 Zhao Y, Marotta M, Eichler EE, Eng C, Tanaka H (2009). Linkage disequilibrium between two high-frequency deletion polymorphisms: implications for association studies involving the glutathione-S transferase (GST) genes. PLoS Genet, 5, 1000472.   DOI   ScienceOn
5 Lee SA, Kim JW, Roh JW, et al (2004). Genetic polymorphisms of GSTM1, p21, p53 and HPV infection with cervical cancer in Korean women. Gynecol Oncol, 93, 14-8.   DOI   ScienceOn
6 Mainwaring GW, Williams SM, Foster JR, Tugwood J, Green T (1996). The distribution of theta-class glutathione S-transferases in the liver and lung of mouse, rat and human. Biochem J, 318, 297-303.
7 Rotunno M, Lam TK, Vogt A, et al (2012). GSTM1 and GSTT1 copy numbers and mRNA expression in lung cancer. Mol Carcinog, 51, 142-50.   DOI   ScienceOn
8 Singh H, Sachan R, Devi S, Pandey SN, Mittal B (2008). Association of GSTM1, GSTT1 and GSTM3 gene polymorphisms and susceptibility to cervical cancer in a North Indian population. Am J Obstet Gynecol, 198, 1-6.   DOI   ScienceOn
9 Sugai T, Habano W, Jiao YF, et al (2006). Analysis of molecular alterations in left-and right-sided colorectal carcinomas reveals distinct path ways of carcinogenesis: proposal for new molecular profile of colorectal carcinomas. J Mol Diagn, 8, 193-201.   DOI   ScienceOn
10 Talalay P (2000). Chemoprotection against cancer by induction of phase 2 enzymes. Biofactors, 12, 5-11.   DOI
11 Tunali NE, Tiryakioglu NO (2011). Polymorphisms in the xenobiotic genes and susceptibility to bladder cancer. J Cell and Molecular Biology, 9, 5-13.
12 van Eijl S, Zhu Z, Cupitt J, et al (2012). Elucidation of xenobiotic metabolism pathways in human skin and human skin models by proteomic profiling. PloS one, 7, 41721.   DOI   ScienceOn
13 Whiteside TL (2008). The tumor microenvironment and its role in promoting tumor growth. Oncogene, 27, 5904-12.   DOI   ScienceOn
14 Economopoulos KP, Sergentanis TN (2010). GSTM1, GSTT1, GSTP1, GSTA1 and colorectal cancer risk: a comprehensive meta-analysis. Eur J Cancer, 46, 1617-31.   DOI   ScienceOn
15 Ferlay J, Shin HR, Bray F, et al (2010). Colorectal cancer incidence, mortality and prevalence worldwide in 2008-summary. Available from: GLOBOCAN 2008 v2.0, Cancer Incidence and Mortality Worldwide: IARC CancerBase No. 10. Lyon, France: Int J Cancer, 127, 2893-917.   DOI   ScienceOn
16 Dialyna IA, Arvanitis DA, Spandidos DA (2001). Genetic polymorphisms and transcriptional pattern analysis of CYP1A1, AhR, GSTM1, GSTP1 and GSTT1 genes in breast cancer. Int J Mol Med, 8, 79-87.
17 Diedrich A, Bock HC, Konig F, et al (2006). Expression of glutathione S-transferase T1 (GSTT1) in human brain tumours. Histol Histopathol, 21, 1199-207.
18 Glebov OK, Rodriguez LM, Nakahara K, et al (2003). Distinguishing right from left colon by the pattern of gene expression. Cancer Epidemiol Biomarkers Prev, 12, 755-62.
19 Grootjans J, Thuijls G, Verdam F, et al (2010). Non-invasive assessment of barrier integrity and function of the human gut. World J Gastrointest Surg, 2, 61.
20 Ha YS, Yan C, Park C, et al (2011). GSTT1: a marker of the aggressiveness of bladder cancer. Urol Int, 86, 41-6.   DOI   ScienceOn
21 Hayes JD, Pulford DJ (1995). The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. 18. Crit Rev Biochem Mol Biol, 30, 445-600.   DOI   ScienceOn
22 Ito M, Imai M, Muraki M, et al (2011). GSTT1 is upregulated by oxidative stress through p38-MK2 signaling pathway in human granulosa cells: possible association with mitochondrial activity. Aging, 3, 1213-23.
23 de Bruin WC, Wagenmans MJ, Board PG, Peters WH (1999). Expression of glutathione S-transferase theta class isoenzymes in human colorectal and gastric cancers. Carcinogenesis, 20, 1453-7.   DOI   ScienceOn
24 Chen S-H, Wu W-J, Tu H-P, et al (2013). Glutathione S-transferase expression in upper urinary tract urothelial carcinomas: a Taiwan study. Asian Pac J Cancer Prev, 14, 6475-9-84.   과학기술학회마을   DOI   ScienceOn
25 Cotton SC, Sharp L, Little J, Brockton N (2000). Glutathione S-transferase polymorphisms and colorectal cancer: a huge review. Am J Epidemiol, 151, 7-32.   DOI   ScienceOn