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http://dx.doi.org/10.7314/APJCP.2014.15.4.1871

Ethanolic Extract from Derris scandens Benth Mediates Radiosensitzation via Two Distinct Modes of Cell Death in Human Colon Cancer HT-29 Cells  

Hematulin, Arunee (Radiobiology Research Unit, Department of Radiation Technology, Faculty of Allied Health Sciences)
Ingkaninan, Kornkanok (Department of Pharmaceutical Chemistry and Pharmacognosy, Naresuan University)
Limpeanchob, Nanteetip (Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Naresuan University)
Sagan, Daniel (Radiobiology Research Unit, Department of Radiation Technology, Faculty of Allied Health Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.4, 2014 , pp. 1871-1877 More about this Journal
Abstract
Enhancing of radioresponsiveness of tumors by using radiosensitizers is a promising approach to increase the efficacy of radiation therapy. Recently, the ethanolic extract of the medicinal plant, Derris scandens Benth has been identified as a potent radiosensitizer of human colon cancer HT29 cells. However, cell death mechanisms underlying radiosensitization activity of D scandens extract have not been identified. Here, we show that treatment of HT-29 cells with D scandens extract in combination with gamma irradiation synergistically sensitizes HT-29 cells to cell lethality by apoptosis and mitotic catastrophe. Furthermore, the extract was found to decrease Erk1/2 activation. These findings suggest that D scandens extract mediates radiosensitization via at least two distinct modes of cell death and silences pro-survival signaling in HT-29 cells.
Keywords
Radiosensitizer; Derris scandens benth; radiotherapy; apotosis mitotic catastrophe;
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