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http://dx.doi.org/10.7314/APJCP.2014.15.3.1255

Association of Benign Prostate Hyperplasia with Polymorphisms in VDR, CYP17, and SRD5A2 Genes among Lebanese Men  

El Ezzi, Asmahan Ali (Radioimmunoassay Laboratory, Lebanese Atomic Energy Commission)
Zaidan, Wissam Rateeb (Radioimmunoassay Laboratory, Lebanese Atomic Energy Commission)
El-Saidi, Mohammed Ahmed (Office of Research, Utah Valley University)
Al-Ahmadieh, Nabil (Department of Urology, Mount Lebanon Hospital)
Mortenson, Jeffrey Benjamin (Department of Biology, Utah Valley University)
Kuddus, Ruhul Haque (Department of Biology, Utah Valley University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.3, 2014 , pp. 1255-1262 More about this Journal
Abstract
Background: The aim of the study was to investigate any associations between benign prostate hyperplasia (BPH) and single nucleotide polymorphisms (SNPs) in the VDR gene (FokI, BsmI, ApaI and Taq${\alpha}$I loci) and the CYP17 gene (MspA1I locus), as well as TA repeat polymorphism in SRD5A2 gene among Lebanese men. Materials and Methods: DNA extracted from blood of 68 subjects with confirmed BPH and 79 age-matched controls was subjected to PCR/PCR-restriction fragment length polymorphism analysis. The odds ra=tio (OR) of having a genotype and the relative risk (RR) of developing BPH for having the genotype were calculated and the alleles were designated risk-bearing or protective. Results: Our data indicated that the A and B alleles of the VDR ApaI and BsmI SNPs were highly associated with increased risk of BPH (p=0.0168 and 0.0002, respectively). Moreover, 63% of the controls compared to 43% of the subjects with BPH were homozygous for none of the risk-bearing alleles (p=0.0123) whereas 60% of the controls and 28% of the subjects with BPH were homozygous for two or more protective alleles (p<0.0001). Conclusions: For the first time, our study demonstrated that ApaI and BsmI of the VDR gene are associated with risk of BPH among Lebanese men. Our study also indicated that overall polymorphism profile of all the genes involved in prostate physiology could be a better predictor of BPH risk.
Keywords
Benign prostate hyperplasia (BPH); single nucleotide polymorphism (SNP); TA repeat polymorphism;
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