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http://dx.doi.org/10.7314/APJCP.2014.15.3.1181

Promoter Polymorphisms of ST3GAL4 and ST6GAL1 Genes and Associations with Risk of Premalignant and Malignant Lesions of the Cervix  

de los Angeles Rivera-Juarez, Maria (Laboratorio de Biologia Molecular y Virologia, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Rosas-Murrieta, Nora Hilda (Centro de Quimica, Instituto de Ciencias, Benemerita Universidad Autonoma de Puebla)
Mendieta-Carmona, Victoriano (Laboratorio de Biologia Molecular y Virologia, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Hernandez-Pacheco, Raquel Esneidy (Facultad de Medicina, Benemerita Universidad Autonoma de Puebla)
Zamora-Ginez, Irma (Facultad de Medicina, Benemerita Universidad Autonoma de Puebla)
Rodea-Avila, Carlos (Hospital de Oncologia, UMAE Hospital de Oncologia. Centro Medico Nacional Siglo XXI)
Apresa-Garcia, Teresa (Unidad de Investigacion en Enfermedades Oncologicas, UMAE Hospital de Oncologia. Centro Medico Nacional Siglo XXI)
Garay-Villar, Onix (Servicio de Radioterapia, Area de Braquiterapia, UMAE Hospital de Oncologia. Centro Medico Nacional Siglo XXI)
Aguilar-Lemarroy, Adriana (Division de Inmunologia, Centro de Investigacion Biomedica de Occidente (CIBO))
Jave-Suarez, Luis Felipe (Division de Inmunologia, Centro de Investigacion Biomedica de Occidente (CIBO))
Diaz-Orea, Maria Alicia (Facultad de Medicina, Benemerita Universidad Autonoma de Puebla)
Milflores-Flores, Lorena (Escuela de Biologia, Benemerita Universidad Autonoma de Puebla)
Reyes-Salinas, Juan Salvador (Servicio de Radioterapia, Hospital de Especialidades)
Ceja-Utrera, Francisco Javier (Servicio de Radioterapia, Hospital de Especialidades)
Vazquez-Zamora, Victor Javier (Servicio de Radioterapia, Hospital de Especialidades)
Vargas-Maldonado, Tomas (Banco de Sangre Hospital, Hospital de Especialidades)
Reyes-Carmona, Sandra (Centro de Quimica, Instituto de Ciencias, Benemerita Universidad Autonoma de Puebla)
Sosa-Jurado, Francisca (Laboratorio de Biologia Molecular y Virologia, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Santos-Lopez, Gerardo (Laboratorio de Biologia Molecular y Virologia, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Reyes-Leyva, Julio (Laboratorio de Biologia Molecular y Virologia, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Vallejo-Ruiz, Veronica (Laboratorio de Biologia Molecular y Virologia, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.3, 2014 , pp. 1181-1186 More about this Journal
Abstract
Sialyltransferase gene expression is altered in several cancers, including examples in the cervix. Transcriptional regulation of the responsible genes depends on different promoters. We aimed to determine the association of single-nucleotide polymorphisms in the B3 promoter of the ST3GAL4 gene and the P1 promoter of the ST6GAL1 gene with cervical premalignant lesions or cervical cancer. A blood sample and/or cervical scrapes were obtained from 104 women with normal cytology, 154 with premalignant lesions and 100 with cervical cancer. We also included 119 blood samples of random donors. The polymorphisms were identified by sequencing from PCR products. For the B3 promoter, a fragment of 506 bp (from nucleotide -408 to +98) was analyzed, and for the P1 promoter a 490 bp (-326 to +164) fragment. The polymorphism analysis showed that at SNP rs10893506, genotypes CC and CT of the ST3GAL4 B3 promoter were associated with the presence of premalignant lesions (OR=2.89; 95%CI 1.72-4.85) and cervical cancer (OR=2.23; 95%CI 1.27-3.91). We detected only one allele of each polymorphism in the ST6GAL1 P1 promoter. We did not detect any genetic variability in the P1 promoter region in our study population. Our results suggest that the rs10893506 polymorphism -22C/T may increase susceptibility to premalignant and malignant lesions of the cervix.
Keywords
SNPs, single-nucleotide polymorphism; sialyltransferase; cervical cancer; cervical neoplasia;
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