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http://dx.doi.org/10.7314/APJCP.2014.15.21.9375

A Piezoelectric Immunosensor for Early Cervical Cancer Detection  

Yang, Li (Frequency Control Laboratory, School of Automation and Engineering, University of Electronic Science and Technology of China)
Huang, Xianhe (Frequency Control Laboratory, School of Automation and Engineering, University of Electronic Science and Technology of China)
Sun, Liang (Department of Pathology, West China Second University Hospital, Sichuan University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.21, 2014 , pp. 9375-9378 More about this Journal
Abstract
Background: A piezoelectric immunosensor for early cervical cancer detection was developed involving short analyis time and less invasive technique for $p16^{INK4a}$, a protein that has been linked to cervical cancer. Materials and Methods: $5{\mu}L$ of 5.0 mg/mL $p16^{INK4a}$ antibody and then supernatant from different clinical samples from West China Second University Hospital (Sichuan, China) were dripped on the center of the AT-cut crystal through a micro-injector. Absorption of the $p16^{INK4a}$ by antibody caused a shift in the resonant frequency of the immunosensor, and the resonant frequency was correlated to the amount of the $p16^{INK4a}$ in the supernatant. Results: The greater severity of lesion grading, the greater the expression level of $p16^{INK4a}$. Conclusion: Degree of cervical cancer lesion development could be determined by detected amount of $p16^{INK4a}$ in different clinical samples.
Keywords
Quartz crystal microbalance; immunosensor; early cervical cancer; detection;
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Times Cited By KSCI : 2  (Citation Analysis)
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