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http://dx.doi.org/10.7314/APJCP.2014.15.21.9319

Alkaloids from Beach Spider Lily (Hymenocallis littoralis) Induce Apoptosis of HepG-2 Cells by the Fas-signaling Pathway  

Ji, Yu-Bin (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Chen, Ning (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Zhu, Hong-Wei (State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences)
Ling, Na (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Li, Wen-Lan (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Song, Dong-Xue (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Gao, Shi-Yong (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Zhang, Wang-Cheng (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Ma, Nan-Nan (Engineering Research Center of Natural Anticancer Drugs, Harbin University of Commerce)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.21, 2014 , pp. 9319-9325 More about this Journal
Abstract
Alkaloids are the most extensively featured compounds of natural anti-tumor herbs, which have attracted much attention in pharmaceutical research. In our previous studies, a mixture of major three alkaloid components (5, 6-dihydrobicolorine, 7-deoxy-trans-dihydronarciclasine, littoraline) from Hymenocallis littoralis were extracted, analyzed and designated as AHL. In this paper, AHL extracts were added to human liver hepatocellular cells HepG-2, human gastric cancer cell SGC-7901, human breast adenocarcinoma cell MCF-7 and human umbilical vein endothelial cell EVC-304, to screen one or more AHL-sensitive tumor cell. Among these cells, HepG-2 was the most sensitive to AHL treatment, a very low dose ($0.8{\mu}g/ml$) significantly inhibiting proliferation. The non-tumor cell EVC-304, however, was not apparently affected. Effect of AHL on HepG-2 cells was then explored. We found that the AHL could cause HepG-2 cycle arrest at G2/M checkpoint, induce apoptosis, and interrupt polymerization of microtubules. In addition, expression of two cell cycle-regulated proteins, CyclinB1 and CDK1, was up-regulated upon AHL treatment. Up-regulation of the Fas, Fas ligand, Caspase-8 and Caspase-3 was observed as well, which might imply roles for the Fas/FsaL signaling pathway in the AHL-induced apoptosis of HepG-2 cells.
Keywords
Beach spider lily; AHL; HepG-2; apoptosis; cell arrest; cell cycle-regulated protein; Fas signaling;
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