Browse > Article
http://dx.doi.org/10.7314/APJCP.2014.15.21.9249

Curcumin-loaded PLGA Nanoparticles Conjugated with Anti-P-glycoprotein Antibody to Overcome Multidrug Resistance  

Punfa, Wanisa (Department of Biochemistry, Faculty of Medicine, Chiang Mai University)
Suzuki, Shugo (Department of Experimental Pathology and Tumor Biology Graduate School of Medical Science, Nagoya City University)
Pitchakarn, Pornsiri (Department of Biochemistry, Faculty of Medicine, Chiang Mai University)
Yodkeeree, Supachai (Department of Biochemistry, Faculty of Medicine, Chiang Mai University)
Naiki, Taku (Department of Experimental Pathology and Tumor Biology Graduate School of Medical Science, Nagoya City University)
Takahashi, Satoru (Department of Experimental Pathology and Tumor Biology Graduate School of Medical Science, Nagoya City University)
Limtrakul, Pornngarm (Department of Biochemistry, Faculty of Medicine, Chiang Mai University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.21, 2014 , pp. 9249-9258 More about this Journal
Abstract
Background: The encapsulation of curcumin (Cur) in polylactic-co-glycolic acid (PLGA) nanoparticles (Cur-NPs) was designed to improve its solubility and stability. Conjugation of the Cur-NPs with anti-P-glycoprotein (P-gp) antibody (Cur-NPs-APgp) may increase their targeting to P-gp, which is highly expressed in multidrugresistance (MDR) cancer cells. This study determined whether Cur-NPs-APgp could overcome MDR in a human cervical cancer model (KB-V1 cells) in vitro and in vivo. Materials and Methods: First, we determined the MDR-reversing property of Cur in P-gp-overexpressing KB-V1 cells in vitro and in vivo. Cur-NPs and Cur-NPs-APgp, in the range 150-180 nm, were constructed and subjected to an in vivo pharmacokinetic study compared with Cur. The in vitro and in vivo MDR-reversing properties of Cur-NPs and Cur-NPs-APgp were then investigated. Moreover, the stability of the NPs was determined in various solutions. Results: The combined treatment of paclitaxel (PTX) with Cur dramatically decreased cell viability and tumor growth compared to PTX treatment alone. After intravenous injection, Cur-NPs-APgp and Cur-NPs could be detected in the serum up to 60 and 120 min later, respectively, whereas Cur was not detected after 30 min. Pretreatment with Cur-NPs-APgp, but not with NPs or Cur-NPs, could enhance PTX sensitivity both in vitro and in vivo. The constructed NPs remained a consistent size, proving their stability in various solutions. Conclusions: Our functional Cur-NPs-APgp may be a suitable candidate for application in a drug delivery system for overcoming drug resistance. The further development of Cur-NPs-APgp may be beneficial to cancer patients by leading to its use as either as a MDR modulator or as an anticancer drug.
Keywords
Multidrug resistance; curcumin; nanoparticles; targeting drug delivery;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Abdelwahed W, Degobert G, Stainmesse S, Fessi H (2006). Freeze-drying of nanoparticles: formulation, process and storage considerations. Adv Drug Deliv Rev, 58, 1688-713.   DOI
2 Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB (2007). Bioavailability of curcumin: problems and promises. Mol Pharm, 4, 807-18.   DOI   ScienceOn
3 Anuchapreeda S, Leechanachai P, Smith MM, Ambudkar SV, Limtrakul PN (2002). Modulation of P-glycoprotein expression and function by curcumin in multidrug-resistant human KB cells. Biochem Pharmacol, 64, 573-82.   DOI
4 Bain LJ, McLachlan JB, LeBlanc GA (1997). Structureactivity relationships for xenobiotic transport substrates and inhibitory ligands of P-glycoprotein. Environ Health Perspect, 105, 812-8.   DOI   ScienceOn
5 Burgio DE, Gosland MP, McNamara a PJ (1998). Effects of P-glycoprotein modulators on etoposide elimination and central nervous system distribution. J Pharmacol Exp Ther, 287, 911-7.
6 Chang G (2003). Multidrug resistance ABC transporters. FEBS Letters, 555, 102-5.   DOI   ScienceOn
7 Chearwae W, Anuchapreeda S, Nandigama K, Ambudkar SV, Limtrakul P (2004). Biochemical mechanism of modulation of human P-glycoprotein (ABCB1) by curcumin I, II, and III purified from Turmeric powder. Biochem Pharmacol, 68, 2043-52.   DOI   ScienceOn
8 Cho K, Wang X, Nie S, Chen ZG, Shin DM (2008). Therapeutic nanoparticles for drug delivery in cancer. Clin Cancer Res, 14, 1310-6.   DOI   ScienceOn
9 CIOMS (1985). International guiding principles for biomedical research involving animals. Altern Lab Anim, 12, 2.
10 Cirstoiu-Hapca A, Buchegger F, Lange N, et al (2010). Benefit of anti-HER2-coated paclitaxel-loaded immuno-nanoparticles in the treatment of disseminated ovarian cancer: Therapeutic efficacy and biodistribution in mice. J Control Release, 144, 324-31.   DOI
11 Hu YP, Pourquier P, Doignon F, Crouzet M, Robert J (1996). Effects of modulators of multidrug resistance on the expression of the MDR1 gene on human KB cells in culture. Anticancer Drugs, 7, 738-44.   DOI
12 Cisternino S, Rousselle C, Debray M, Scherrmann JM (2004). In situ transport of vinblastine and selected P-glycoprotein substrates: implications for drug-drug interactions at the mouse blood-brain barrier. Pharm Res, 21, 1382-9.   DOI
13 Dai X-Z, Yin H-T, Sun L-F, et al (2013). Potential therapeutic efficacy of curcumin in liver cancer. Asian Pac J Cancer Prev, 14, 3855-9.   과학기술학회마을   DOI   ScienceOn
14 Gallo JM, Li S, Guo P, Reed K, Ma J (2003). The effect of P-glycoprotein on paclitaxel brain and brain tumor distribution in mice. Cancer Res, 63, 5114-7.
15 Iangcharoen P, Punfa W, Yodkeeree S, et al (2011). Anti-Pglycoprotein conjugated nanoparticles for targeting drug delivery in cancer treatment. Arch Pharm Res, 34, 1679-89.   DOI
16 Jang SH, Wientjes MG, Au JL (2001). Kinetics of P-glycoproteinmediated efflux of paclitaxel. J Pharmacol Exp Ther, 298, 1236-42.
17 Kiziltepe T, Ashley JD, Stefanick JF, et al (2012). Rationally engineered nanoparticles target multiple myeloma cells, overcome cell-adhesion-mediated drug resistance, and show enhanced efficacy in vivo. Blood Cancer J, 2, 64.   DOI
18 Lazzari S, Moscatelli D, Codari F, et al (2012). Colloidal stability of polymeric nanoparticles in biological fluids. J Nanopart Res, 14, 920.   DOI
19 Maeda H, Wu J, Sawa T, Matsumura Y, Hori K (2000). Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review. J Control Release, 65, 271-84.   DOI   ScienceOn
20 Limtrakul P, Khantamat O, Pintha K (2004). Inhibition of P-glycoprotein activity and reversal of cancer multidrug resistance by Momordica charantia extract. Cancer Chemother Pharmacol, 54, 525-30.   DOI
21 Makadia HK, Siegel SJ (2011). Poly lactic-co-glycolic acid (PLGA) as biodegradable controlled drug delivery carrier. Polymers (Basel), 3, 1377-97.   DOI   ScienceOn
22 Manjunath K, Venkateswarlu V (2005). Pharmacokinetics, tissue distribution and bioavailability of clozapine solid lipid nanoparticles after intravenous and intraduodenal administration. J Control Release, 107, 215-28.   DOI   ScienceOn
23 Mechetner EB, Roninson IB (1992). Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A, 89, 5824-8.   DOI
24 Meng H, Mai WX, Zhang H, et al (2013). Codelivery of an optimal drug/siRNA combination using mesoporous silica nanoparticles to overcome drug resistance in breast cancer in vitro and in vivo. ACS Nano, 7, 994-1005.   DOI
25 Ohara Y, Oda T, Yamada K, et al (2012). Effective delivery of chemotherapeutic nanoparticles by depleting host Kupffer cells. Int J Cancer, 131, 2402-10.   DOI
26 Oyagbemi AA, Saba AB, Ibraheem AO (2009). Curcumin: from food spice to cancer prevention. Asian Pac J Cancer Prev, 10, 963-7.
27 Sadauskas E, Wallin H, Stoltenberg M, et al (2007). Kupffer cells are central in the removal of nanoparticles from the organism. Part Fibre Toxicol, 4, 10.   DOI
28 Punfa W, Yodkeeree S, Pitchakarn P, Ampasavate C, Limtrakul P (2012). Enhancement of cellular uptake and cytotoxicity of curcumin-loaded PLGA nanoparticles by conjugation with anti-P-glycoprotein in drug resistance cancer cells. Acta Pharmacol Sin, 33, 823-31.   DOI
29 Qiao WJ, Cheng HY, Li CQ, et al (2011). Identification of pathways involved in paclitaxel activity in cervical cancer. Asian Pac J Cancer Prev, 12, 99-102.
30 Qureshi S, Shah AH, Ageel AM (1992). Toxicity studies on Alpinia galanga and Curcuma longa. Planta Med, 58, 124-7.   DOI   ScienceOn
31 Shehzad A, Wahid F, Lee YS (2010). Curcumin in cancer chemoprevention: molecular targets, pharmacokinetics, bioavailability, and clinical trials. Arch Pharm, 343, 489-99.   DOI
32 Shen F, Chu S, Bence AK, et al (2008). Quantitation of doxorubicin uptake, efflux, and modulation of multidrug resistance (MDR) in MDR human cancer cells. J Pharmacol Exp Ther, 324, 95-102.
33 Song Z, Feng R, Sun M, et al (2011). Curcumin-loaded PLGA-PEG-PLGA triblock copolymeric micelles: Preparation, pharmacokinetics and distribution in vivo. J Colloid Interface Sci, 354, 116-23.   DOI
34 Tabatabaei Mirakabad FS, Nejati-Koshki K, Akbarzadeh A, et al (2014). PLGA-Based Nanoparticles as Cancer Drug Delivery Systems. Asian Pac J Cancer Prev, 15, 517-35.   과학기술학회마을   DOI   ScienceOn
35 Yadav D, Anwar MF, Garg V, et al (2014). Development of polymeric nanopaclitaxel and comparison with free paclitaxel for effects on cell proliferation of MCF-7 and B16F0 carcinoma cells. Asian Pac J Cancer Prev, 15, 2335-40.   과학기술학회마을   DOI   ScienceOn
36 Torchilin VP (2005). Recent advances with liposomes as pharmaceutical carriers. Nat Rev Drug Discov, 4, 145-60.   DOI   ScienceOn
37 Tsai YM, Chien CF, Lin LC, Tsai TH (2011). Curcumin and its nano-formulation: the kinetics of tissue distribution and blood-brain barrier penetration. Int J Pharm, 416, 331-8.   DOI   ScienceOn
38 Wang W, Zhu R, Xie Q, et al (2012). Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles. Int J Nanomedicine, 7, 3667-77.
39 Yamate J, Tatsumi M, Nakatsuji S, et al (1993). Immunohistochemical observations of macrophages and perisinusoidal cells in carbon tetrachloride-induced rat liver injury. J Vet Med Sci, 55, 973-7.   DOI
40 Yin H-T, Zhang D-G, Wu X-L, Huang X-E, Chen G (2013). In vivo evaluation of curcumin-loaded nanoparticles in a A549 xenograft mice model. Asian Pac J Cancer Prev, 14, 409-12.   과학기술학회마을   DOI   ScienceOn
41 Zhang C, Qu G, Sun Y, et al (2008). Pharmacokinetics, biodistribution, efficacy and safety of N-octyl-O-sulfate chitosan micelles loaded with paclitaxel. Biomaterials, 29, 1233-41.   DOI
42 Zhang L, Gu FX, Chan JM, et al (2008). Nanoparticles in medicine: therapeutic applications and developments. Clin Pharmacol Ther, 83, 761-9.   DOI   ScienceOn