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http://dx.doi.org/10.7314/APJCP.2014.15.21.9131

Analysis of the Biological Function of ELDF15 Using an Antisense Recombinant Expression Vector  

Liu, Yan (Chinese Center for Disease Control and Prevention)
Wang, Long (Beijing Laboratory Animal Research Center)
Wang, Zi-Jun (Chinese Center for Disease Control and Prevention)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.21, 2014 , pp. 9131-9136 More about this Journal
Abstract
ELDF15, homologous with AT2 receptor-interaction protein 1 (ATIP1), may play an important role in cell differentiation, proliferation, and carcinogenesis. We aimed to understand the biological function of ELDF15 via construction and transfection of a recombinant expression vector containing antisense ELDF15. Recombinant expression vectors were successfully constructed and transfected into K562 cells. A stable transfectant, known as pXJ41-asELDF15, stably produced antisense ELDF15. Compared with K562 and K562-zeo cells, K562-pXJ41-asELDF15 cells showed inhibition of cell proliferation. RT-PCR analysis showed that the expression and protein level of ELDF15 decreased significantly in K562 cells transfected with pXJ41-asELDF15. Expression of hemoglobin increased in K562 cells transfected with pXJ41-asELDF15 by benzidine staining. increases NBT reduction activity in K562 cells transfected with pXJ41-asELDF15.Colony forming efficiency in two-layer soft agar was clearly inhibited as assessed by electron microscopy. These results suggest that ELDF15 plays a potential role in cell differentiation, proliferation and carcinogenesis.
Keywords
ELDF15; gene; cell proliferation; differentiation; antisense technology; carcinogenesis;
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1 Ali Owchi M, Salehnia M, Moghadam MF, Boroujeni MB, Hajizadeh E (2009). The effect of bone morphogenetic protein 4 on the differentiation of mouse embryonic stem cell to erythroid lineage in serum free and serum supplemented media. Int J Biomed Sci, 5, 275-82.
2 Chen WC, Pan SL (2006). Recent advancement of tumor suppressor genes and their association with cellular senescence. Med Recap, 12, 711-13.
3 Cao YL (2012). Research progress of cancer genes and their detection methods. Life Sci Instr, 10, 10-6
4 Dean JL, Knudsen KE (2013). The role of tumor suppressor dysregulation in prostate cancer progression. Curr Drug Targets, 14, 460-71.   DOI
5 Ding GR, Wang XW, Li KC, et al (2009). Comparison of Hsps expression after r adio-frequency field exposure in three human glioma cell lines. Biomed Environ Sci, 22, 374-80.   DOI
6 Gao HX, Gao XF, Wang GQ, et al (2012). In vitro study of nucleostemin gene as a potential therapeutic target for human lung carcinoma. Biomed Environ Sci, 25, 91-97.
7 Guo XE, Ngo B, Modrek AS, et al (2014.) Targeting tumor suppressor networks for cancer therapeutics. Curr Drug Targets, 15, 2-16.   DOI
8 Horiuchi M, Iwanami J, Mogi M (2012). Regulation of angiotensin II receptors beyond the classical pathway. Clin Sci, 123,193-203.   DOI
9 Kong N, Zhang XM, Wang HT, et al (2013). Inhibition of growth and induction of differentiation of SMMC-7721 human hepatocellular carcinoma cells by oncostatin M. Asian Pac J Cancer Prev, 14, 747-52.   과학기술학회마을   DOI   ScienceOn
10 Liu Y, Zhang SF, Jin SM, et al (2003). Expression and location of a novel gene with cell differentiation in different tissues and cell lines. Acta Laboratorium Animals Scientia Sinica, 11, 85-7.
11 Li J, Meng FL, He LH, Zhang JZ (2012). Secreted protein HP1286 of Helicobacter pylori strain 26695 induces apoptosis of AGS cells. Biomed Environ Sci, 25, 614-19.
12 Liu M, Wu R, Yang F, et al (2013). Identification of FN1BP1 as a novel cell cycle regulator through modulating G1 checkpoint in human hepatocarcinoma hep3B cells. PloS One, 8, 57574.   DOI
13 Niu JY, Liu J, Liu L, et al (2012). Construction of eukaryotic plasmid expressing human TGFBI and its influence on human corneal epithelial cells. Int J Ophthalmol, 5, 38-44.
14 Olin JL, Veverka A, Nuzum DS (2011). Risk of cancer associated with the use of angiotensin II-receptor blockers. Am J Health Syst Pharm, 68, 2139-46.   DOI
15 PylayevaGupta Y, Grabocka E, BarSagi D (2011). RAS oncogenes: weaving a tumorigenic web. Nat Rev Cancer, 11, 761-74.   DOI   ScienceOn
16 Pappou EP, Ahuja N (2010). The role of oncogenes in gastrointestinal cancer. Gastrointest Cancer Res, 1, 2-15.
17 Heroor S, Beknal AK, Mahurkar N (2013). Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. In mice and on human neutrophils. Indian J Pharmacol, 45, 130-35.   DOI
18 Sankari SL, Masthan KM, Babu NA, Bhattacharjee T, Elumalai M (2012). Apoptosis in cancer--an update. Asian Pac J Cancer Prev, 13, 7873-8.
19 Taylor Tavares AL, Willatt L, Armstrong R, et al (2013). Mosaic deletion of the NF1 gene in a patient with cognitive disability and dysmorphic features but without diagnostic features of NF1. Am J Med Genet A, 161, 1185-8.   DOI
20 Tao HC, Wang HX, Dai M, et al (2013). Targeting SHCBP1 inhibits cell proliferation in human hepatocellular carcinoma cells. Asian Pac J Cancer Prev, 14, 5645-50.   과학기술학회마을   DOI
21 Wei YY, Hou J, Tang WR, Luo Y (2012). The cooperation between p53 and Ras in tumorigenesis. Yi Chuan, 34, 1513-21.   DOI
22 Wang J, Lv XW, Du YG (2009). Potential mechanisms involved in ceramide-induced apoptosis in human colon cancer HT29 cells. Biomed Environ Sci, 22, 76-85.   DOI
23 Wang S, Wang Z (2013). Epigenetic aberrant methylation of tumor suppressor genes in small cell lung cancer. J Thorac Dis, 5, 532-7.
24 Yong Huang, Quan Zou, Sheng-peng Wang, et al (2011). Construction and detection of expression vectors of microRNA-9a in BmN cells. J Zhejiang Univ Sci B, 12, 527-33.   DOI