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http://dx.doi.org/10.7314/APJCP.2014.15.1.257

Association of +405C>G and +936C>T Polymorphisms of the Vascular Endothelial Growth Factor Gene with Sporadic Breast Cancer in North Indians  

Kapahi, Ruhi (Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University)
Manjari, Mridu (Department of Pathology, Sri Guru Ram Das Institute of Medical Sciences and Research)
Sudan, Meena (Department of Radiotherapy, Sri Guru Ram Das Institute of Medical Sciences and Research)
Uppal, Manjit Singh (Department of Surgery, Sri Guru Ram Das Institute of Medical Sciences and Research)
Singh, Neeti Rajan (Department of Surgery, Sri Guru Ram Das Institute of Medical Sciences and Research)
Sambyal, Vasudha (Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University)
Guleria, Kamlesh (Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.1, 2014 , pp. 257-263 More about this Journal
Abstract
Background: Vascular endothelial growth factor (VEGF), an endothelial cell specific mitogen, has been implicated as a critical factor influencing tumor related angiogenesis. The aim of present study was to evaluate the relationship between VEGF +936C>T and +405C>G polymorphisms of VEGF with risk of breast cancer in Punjab, India. Materials and Methods: We screened DNA samples of 192 sporadic breast cancer patients and 192 unrelated healthy, gender and age matched control individuals for VEGF +936C>T and +405C>G polymorphisms using the PCR-RFLP method. Results: For the VEGF +405C>G polymorphism, we observed significantly increased frequency of GG genotype in cases as compared to controls and strong association of +405GG genotype was observed with three fold risk for breast cancer (OR=3.07; 95%CI 1.41-6.65; p=0.003). For the +936C>T polymorphism, significant associations of CT and combined CT+TT genotypes were observed with elevated risk of breast cancer (p=0.021; 0.023). The combined genotype combinations of GG-CC and GG-CT of +405C>G and +936C>T polymorphisms were found to be significantly associated with increased risk of breast cancer (p=0.04; 0.0064). Conclusions: The findings of the present study indicated significant associations of VEGF +936C>T and +405C>G polymorphisms with increased breast cancer risk in patients from Punjab, North India.
Keywords
Breast cancer; VEGF; polymorphisms; angiogenesis; Punjab, North India;
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1 Zhai R, Liu G, Asomaning K, et al (2008b). Genetic polymorphisms of VEGF, interactions with cigarette smoking exposure and esophageal adenocarcinoma risk. Carcinogenesis, 29, 2330-4.   DOI
2 Zhang B, Beeghly-Fadiel A, Lu W, et al (2011). Evaluation of functional genetic variants for breast cancer risk: results from the Shanghai breast cancer study. Am J Epidemiol, 173, 1159-70.   DOI
3 Zhao Z, Fu YX, Hewett-Emmett D, et al (2003). Investigating single nucleotide polymorphism (SNP) density in the human genome and its implications for molecular evolution. Gene, 312, 207-13.   DOI
4 Tzanakis N, Gazouli M, Rallis G, et al (2006). Vascular endothelial growth factor polymorphisms in gastric cancer development, prognosis, and survival. J Surg Oncol, 94, 624-30.   DOI   ScienceOn
5 Stevens A, Soden J, Brenchley PE, et al (2003). Haplotype analysis of the polymorphic human vascular endothelial growth factor gene promoter. Cancer Res, 63, 812-6.
6 Talar-Wojnarowska R, Gasiorowska A, Olakowski M, et al (2010). Vascular endothelial growth factor (VEGF) genotype and serum concentration in patients with pancreatic adenocarcinoma and chronic pancreatitis. J Physiol Pharmacol, 61, 711-6.
7 Tischer E, Mitchell R, Hartman T, et al (1991). The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. J Biol Chem, 266, 11947-54.
8 Watson CJ, Webb NJ, Bottomley MJ, et al (2000). Identification of polymorphisms within the vascular endothelial growth factor (VEGF) gene: correlation with variation in VEGF protein production. Cytokine, 12, 1232-5.   DOI   ScienceOn
9 Wolf G, Aigner RM, Schaffler G, et al (2004). The 936C>T polymorphism of the gene for vascular endothelial growth factor is associated with 18F-fluorodeoxyglucose uptake. Breast Cancer Res Treat, 88, 205-8.   DOI
10 Yapijakis C, Vairaktaris E, Vassiliou S, et al (2007). The low VEGF production allele of the +936C/T polymorphism is strongly associated with increased risk for oral cancer. J Cancer Res Clin Oncol, 133, 787-91.   DOI
11 Zhai R, Liu G, Zhou W, et al (2008a). Vascular endothelial growth factor genotypes, haplotypes, gender, and the risk of non-small cell lung cancer. Clin Cancer Res, 14, 612-7.   DOI
12 Rodrigues P, Furriol J, Tormo, E, et al (2012). The singlenucleotide polymorphisms +936 C/T VEGF and-710 C/T VEGFR1 are associated with breast cancer protection in a Spanish population. Breast Cancer Res Treat, 133, 769-78.   DOI   ScienceOn
13 Pharoah PD, Antoniou A, Bobrow M, et al (2002). Polygenic susceptibility to breast cancer and implications for prevention. Nat Genet, 31, 33-6.   DOI   ScienceOn
14 Ponder BA (2001). Cancer genetics. Nature, 411, 336-41.   DOI   ScienceOn
15 Renner W, Kotschan S, Hoffmann C, et al (2000). A common 936 C/T mutation in the gene for vascular endothelial growth factor is associated with vascular endothelial growth factor plasma levels. J Vasc Res, 37, 443-8.   DOI   ScienceOn
16 Schoppmann SF, Horvat R, Birner P (2002). Lymphatic vessels and lymphangiogenesis in female cancer: mechanisms, clinical impact and possible implications for antilymphangiogenic therapies (Review). Oncol Rep, 9, 455-60.
17 Roy H, Bhardwaj S, Yla-Herttuala S (2006). Biology of vascular endothelial growth factors. FEBS Lett, 580, 2879-87.   DOI   ScienceOn
18 Ruzzo A, Graziano F, Kawakami K, et al (2006). Pharmacogenetic profiling and clinical outcome of patients with advanced gastric cancer treated with palliative chemotherapy. J Clin Oncol, 24, 1883-91.   DOI   ScienceOn
19 Schneider BP, Radovich M, Sledge GW, et al (2008). Association of polymorphisms of angiogenesis genes with breast cancer. Breast Cancer Res Treat, 111, 157-63.   DOI   ScienceOn
20 Sfar S, Hassen E, Saad H, et al (2006). Association of VEGF genetic polymorphisms with Prostate carcinoma risk and clinical outcome. Cytokine, 35, 21-8.   DOI
21 Sivaprasad S, Govardhan B, Harithakrishna R, et al (2013). Association of vascular endothelial growth factor (VEGF) gene polymorphism and increased serum VEGF concentration with pancreatic adenocarcinoma. Pancreatol, 13, 267-72.   DOI
22 Morabito A, Sarmiento R, Bonginelli P, et al (2004). Antiangiogenic strategies, compounds, and early clinical results in breast cancer. Crit Rev Oncol Hematol, 49, 91-107.   DOI
23 Luo T, Chen L, He P, et al (2013). Vascular endothelial growth factor (VEGF) gene polymorphisms and breast cancer risk in a Chinese population. Asian Pac J Cancer Prev, 14, 2433-7.   과학기술학회마을   DOI   ScienceOn
24 Maltese P, Canestrari E, Ruzzo A, et al (2009). VEGF gene polymorphisms and susceptibility to colorectal cancer disease in Italian population. Int J Colorectal Dis, 24, 165-70.   DOI
25 Oliveira C, Lourenco GJ, Silva PM, et al (2011). Polymorphisms in the 5'-and 3'-untranslated region of the VEGF gene and sporadic breast cancer risk and clinicopathologic characteristics. Tumour Biol, 32, 295-300.   DOI   ScienceOn
26 Naik NA, Bhat IA, Afroze D, et al (2012). Vascular endothelial growth factor A gene (VEGFA) polymorphisms and expression of VEGFA gene in lung cancer patients of Kashmir Valley (India). Tumor Biol, 33, 833-9.   DOI
27 Nathanson KL, Weber BL (2001). "Other" breast cancer susceptibility genes: searching for more holy grail. Hum Mol Genet, 10, 715-20.   DOI
28 Nikzamir A, Esteghamati A, Hammedian AA, et al (2012). The role of vascular endothelial growth factor +405 G/C polymorphism and albuminuria in patients with type 2 diabetes mellitus. Mol Biol Rep, 39, 881-6.   DOI
29 Peyromaure M, Camparo P, Badoual C, et al (2007).The expression of vascular endothelial growth factor is associated with the risk of cancer progression after radical Prostatectomy. BJU Int, 99, 1150-3.   DOI   ScienceOn
30 Koukourakis MI, Papazoglou D, Giatromanolaki A, et al (2004). VEGF gene sequence variation defines VEGF gene expression status and angiogenic activity in non-small cell lung cancer. Lung Cancer, 46, 293-8.   DOI   ScienceOn
31 Kawakami M, Furuhata T, Kimura Y, et al (2003). Expression analysis of vascular endothelial growth factors and their relationships to lymph node metastasis in human colorectal cancer. J Exp Clin Cancer Res, 22, 229-37.
32 Kerbel RS (2008). Tumor angiogenesis. N Engl J Med, 358, 2039-49.   DOI   ScienceOn
33 Kim JG, Sohn SK, Chae YS, et al (2007). Vascular endothelial growth factor gene polymorphisms associated with prognosis for patients with gastric cancer. Ann Oncol, 18, 1030-6.   DOI
34 Lee SJ, Lee SY, Jeon HS, et al (2005). Vascular endothelial growth factor gene polymorphisms and risk of primary lung cancer. Cancer Epidemiol Biomarkers Prev, 14, 571-5.   DOI   ScienceOn
35 Krippl P, Langsenlehner U, Renner W, et al (2003). A common 936 C/T gene polymorphism of vascular endothelial growth factor is associated with decreased breast cancer risk. Int J Cancer, 106, 468-71.   DOI   ScienceOn
36 Lachheb J, Chelbi H, Ben Dhifallah I, et al (2008). Association of vascular endothelial growth factor polymorphisms with asthma in Tunisian children. Gene Regul Syst Bio, 2, 89-96.
37 Langsenlehner U, Wolf G, Langsenlehner T, et al (2008). Genetic polymorphisms in the vascular endothelial growth factor gene and breast cancer risk. The Austrian "tumor of breast tissue: incidence, genetics, and environmental risk factors" study. Breast Cancer Res Treat, 109, 297-304.   DOI
38 Lu H, Shu XO, Cui Y, et al (2005). Association of genetic polymorphisms in the VEGF gene with breast cancer survival. Cancer Res, 65, 5015-9.   DOI   ScienceOn
39 Jakubowska A, Gronwald J, Menkiszak J, et al (2008). The VEGF +936 C>T 3'UTR polymorphism reduces BRCA1-associated breast cancer risk in Polish women. Cancer Lett, 262, 71-6.   DOI   ScienceOn
40 Ishigami SI, Arii S, Furutani M, et al (1998). Predictive value of vascular endothelial growth factor (VEGF) in metastasis and prognosis of human colorectal cancer. Br J Cancer, 78, 1379-84.   DOI   ScienceOn
41 Jacobs EJ, Feigelson HS, Bain EB, et al (2006). Polymorphisms in the vascular endothelial growth factor gene and breast cancer in the Cancer Prevention Study II cohort. Breast Cancer Res, 8, 22.
42 Kariyazono H, Ohno T, Khajoee V, et al (2004). Association of vascular endothelial growth factor (VEGF) and VEGF receptor gene polymorphisms with coronary artery lesions of Kawasaki disease. Pediatr Res, 56, 953-9.   DOI   ScienceOn
43 Jain L, Vargo CA, Danesi R, et al (2009). The role of vascular endothelial growth factor SNPs as predictive and prognostic markers for major solid tumors. Mol Cancer Ther, 8, 2496-508.   DOI   ScienceOn
44 Jemal A, Bray F, Center, MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90.   DOI
45 Jin Q, Hemminki K, Enquist K, et al (2005). Vascular endothelial growth factor polymorphisms in relation to breast cancer development and prognosis. Clin Cancer Res, 11, 3647-53.   DOI   ScienceOn
46 Kataoka N, Cai Q, Wen W, et al (2006). Population-based casecontrol study of VEGF gene polymorphisms and breast cancer risk among Chinese women. Cancer Epidemiol Biomarkers Prev, 15, 1148-52.   DOI   ScienceOn
47 Folkman J (2002). Role of angiogenesis in tumor growth and metastasis. Semin Oncol, 29, 15-8.
48 Cooke RW, Drury JA, Mountford R, (2004). Genetic polymorphisms and retinopathy of prematurity. Invest Ophthalmol Vis Sci, 45, 1712-5.   DOI
49 Eroglu A, Ozturk A, Cam R, et al (2008). Vascular endothelial growth factor gene 936 C/T polymorphism in breast cancer patients. Med Oncol, 25, 54-5.   DOI   ScienceOn
50 Ferlay J, Shin HR, Bray F, et al (2008). GLOBOCAN 2008 v2.0, Cancer Incidence and Mortality Worldwide: IARC CancerBase No. 10. Lyon, France: International Agency for Research on Cancer; 2010.
51 Gentilini D, Somigliana E, Vigano P, et al (2008). The vascular endothelial growth factor +405G>C polymorphism in endometriosis. Hum Reprod, 23, 211-5.
52 Hefler LA, Mustea A, Konsgen D, et al (2007). Vascular endothelial growth factor gene polymorphisms are associated with prognosis in ovarian cancer. Clin Cancer Res, 13, 898-901.   DOI   ScienceOn
53 Gerger A, Langsenlehner U, Renner W, et al (2007). A multigenic approach to predict breast cancer risk. Breast Cancer Res Treat, 104, 159-64.   DOI   ScienceOn
54 Guan X, Zhao H, Niu J, et al (2009). The VEGF-634G>C promoter polymorphism is associated with risk of gastric cancer. BMC Gastroenterol, 9, 77   DOI
55 Hamedian AA, Esteghamati A, Noshad S, et al (2012). Vascular endothelial growth factor (VEGF) +405 C/G polymorphism is associated with essential hypertension in a population from Tehran of Iran. Mol Biol Rep, 39, 6213-8.   DOI
56 Hicklin DJ, Ellis LM (2005). Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis. J Clin Oncol, 23, 1011-27.
57 Huez I, Bornes S, Bresson D, Creancier L, Prats H (2001). New vascular endothelial growth factor isoform generated by internal ribosome entry site-driven CUG translation initiation. Mol Endocrinol, 15, 2197-210.   DOI
58 Blackwood MA, Weber BL (1998). BRCA1 and BRCA2: from molecular genetics to clinical medicine. J Clin Oncol, 16, 1969-77.
59 Bao G, Wang M, Guo S, et al (2011). Vascular endothelial growth factor +936 C/T gene polymorphism and glioma risk in a Chinese Han population. Genet Test Mol Biomarkers, 15, 103-6.   DOI
60 Bastide A, Karaa Z, Bornes S, et al (2008). An upstream open reading frame within an IRES controls expression of a specific VEGF-A isoform. Nucleic Acids Res, 36, 2434-45.   DOI
61 Chae YS, Kim JG, Sohn SK, et al (2008). Association of vascular endothelial growth factor gene polymorphisms with susceptibility and clinicopathologic characteristics of colorectal cancer. J Korean Med Sci, 23, 421-7.   DOI
62 Breast cancer facts and figures 2011-2012. http://www.cancer.org/research/cancerfactsstatistics/index. Accessed 9 September 2013
63 Carmeliet P (2005). VEGF as a key mediator of angiogenesis in cancer. Oncol, 69, 4-10.   DOI   ScienceOn
64 Chae YS, Kim JG, Sohn SK, et al (2006). Investigation of vascular endothelial growth factor gene polymorphisms and its association with clinicopathologic characteristics in gastric cancer. Oncol, 71, 266-72.   DOI   ScienceOn
65 Bhanoori M, Arvind Babu K, Pavankumar Reddy NG, et al (2005). The vascular endothelial growth factor (VEGF) +405G>C 5'-untranslated region polymorphism and increased risk of endometriosis in South Indian women: a case control study. Hum Reprod, 20, 1844-9.   DOI
66 Adeli K, Ogbonna G (1990). Rapid purification of human DNA from whole blood for potential application in clinical chemistry laboratories. Clin Chem, 36, 261-4.
67 Bae SJ, Ahn DH, Hong SP, et al (2008). Gender-specific association between polymorphism of vascular endothelial growth factor (VEGF 936C>T) gene and patients with stomach cancer. Yonsei Med J, 49, 783-91.   DOI   ScienceOn
68 Balasubramanian SP, Cox A, Cross SS, et al (2007). Influence of VEGF-A gene variation and protein levels in breast cancer susceptibility and severity. Int J Cancer, 121, 1009-16.   DOI   ScienceOn
69 Wooster R, Bignell G, Lancaster J, et al (1995). Identification of the breast cancer susceptibility gene BRCA2. Nature, 378, 789-92.   DOI   ScienceOn
70 Miki Y, Swensen J, Shattuck-Eidens D, et al (1994). A strong candidate for the breast and ovarian cancer susceptibility gene BRCA1. Science, 266, 66-71.   DOI