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http://dx.doi.org/10.7314/APJCP.2014.15.12.5023

Inactivated Sendai Virus Strain Tianjin Induces Apoptosis in Human Breast Cancer MDA-MB-231 Cells  

Chen, Jun (Department of Microbiology, Basic Medical College, Tianjin Medical University)
Han, Han (Department of Microbiology, Basic Medical College, Tianjin Medical University)
Chen, Min (Department of Nursing, The 44th Hospital of PLA)
Xu, Xiao-Zhu (The Second People Hospital of Guizhou Province)
Wang, Bin (Department of Anesthesiology, Tianjin Research Institute of Anesthesiology, Tianjin Medical University General Hospital)
Shi, Li-Ying (Department of Microbiology, Basic Medical College, Tianjin Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.12, 2014 , pp. 5023-5028 More about this Journal
Abstract
Sendai virus strain Tianjin is a novel genotype. Here, we investigate the antitumor and proapoptotic effects of ultraviolet-inactivated Sendai virus strain Tianjin (UV-Tianjin) on human breast cancer MDA-MB-231 cells in vitro, as well as the involvement of the apoptotic pathway in the mechanism of UV-Tianjin-induced antitumor effects. MTT assays showed that treatment with UV-Tianjin dose-dependently inhibited the proliferation of MDA-MB-231 cells but not normal MCF 10A breast epithelium cells. Hoechst staining and flow cytometric analysis revealed that UV-Tianjin induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Moreover, UV-Tianjin treatment resulted in reduction in the mitochondria membrane potential (MMP) and release of cytochrome complex (cyt c) via regulation of Bax and Bcl-2, as well as activation of caspase-9, caspase-3, Fas, FasL and caspase-8 in MDA-MB-231 cells. In summary, our study suggests that UV-Tianjin exhibits anticancer activity in human breast cancer MDA-MB-231 cells through inducing apoptosis, which may involve both the endogenous mitochondrial and exogenous death receptor pathways.
Keywords
Sendai virus strain Tianjin; human breast cancer MDA-MB-231 cells; apoptosis; caspase;
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