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http://dx.doi.org/10.7314/APJCP.2014.15.10.4255

Lactobacillus acidophilus and Lactobacillus crispatus Culture Supernatants Downregulate Expression of Cancer-testis Genes in the MDA-MB-231 Cell Line  

Azam, Rosa (Department of Medical Genetics, Tehran University of Medical Science)
Ghafouri-Fard, Soudeh (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences)
Tabrizi, Mina (Department of Medical Genetics, Tehran University of Medical Science)
Modarressi, Mohammad-Hossein (Department of Medical Genetics, Tehran University of Medical Science)
Ebrahimzadeh-Vesal, Reza (Department of Medical Genetics, Tehran University of Medical Science)
Daneshvar, Maryam (Department of Medical Genetics, Tehran University of Medical Science)
Mobasheri, Maryam Beigom (Cancer Research Center-Cancer Institute, Tehran University of Medical Science)
Motevaseli, Elahe (Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Science)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.10, 2014 , pp. 4255-4259 More about this Journal
Abstract
Lactobacilli are probiotics shown to have antitumor activities. In addition, they can regulate gene expression through epigenetic mechanisms. In this study, we aimed to assess anti tumor activities of Lactobacillus acidophilus and Lactobacillus crispatus on the MDA-MB-231 breast cancer cell line. The effects of culture supernatants were determined by MTT [3-(4,5-dimethylthiazol-2-y-2,5-diphenyltetrazolium bromide] assay. Changes in expression of 5 cancer-testis antigens (CTAs), namely AKAP4, ODF4, PIWIL2, RHOXF2 and TSGA10, were analyzed by quantitative real time RT-PCR. The culture supernatants of the 2 lactobacilli inhibited MDA-MB-231 cell proliferation. In addition, transcriptional activity of all mentioned CTAs except AKAP4 was significantly decreased after 24 hour treatment with culture supernatants. This study shows that Lactobacillus acidophilus and Lactobacillus crispatus have antiproliferative activity against MDA-MB-231 cells. In addition, these lactobacilli could decrease transcriptional activity of 4 CTAs. Previous studies have shown that expression of CTAs is epigenetically regulated, so it is possible that lactobacilli cause this expression downregulation through epigenetic mechanisms. As expression of CTAs in cancers is usually associated with higher grades and poor prognosis, downregulation of their expression by lactobacilli may have clinical implications.
Keywords
MDA-MB-231; Lactobacillus acidophilus; Lactobacillus crispatus; cancer-testis antigens;
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