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http://dx.doi.org/10.7314/APJCP.2013.14.6.3499

MicroRNA-497 Suppresses Proliferation and Induces Apoptosis in Prostate Cancer Cells  

Wang, Li (Department of Urology, Affiliated Hospital of Hebei, University of Engineering)
Li, Bo (Department of Urology, Affiliated Hospital of Hebei, University of Engineering)
Li, Lei (Department of General Surgery, Affiliated Hospital of Hebei, University of Engineering)
Wang, Te (School of Government of Beijing Normal University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.6, 2013 , pp. 3499-3502 More about this Journal
Abstract
MicroRNAs (miRNAs) are a class of endogenously expressed small, non-coding, single-stranded RNAs that negatively regulate gene expression, mainly by binding to 3'- untranslated regions (3'UTR) of their target messenger RNAs (mRNAs), which cause blocks of translation and/or mRNA cleavage. Recently, miRNAprofiling studies demonstrated the microRNA-497 (miR-497) level to be down-regulated in all prostate carcinomas compared with BPH samples. The purpose of this study was to investigate the potential role of miR-497 in human prostate cancer. Proliferation, cell cycle and apoptosis assays were conducted to explore the potential function of miR-497 in human prostate cancer cells. Results showed that miR-497 suppressed cellular growth and initiated G0/G1 phase arrest of LNCaP and PC-3 cells. We also observed that miR-497 increased the percentage of apoptotic cells by increasing caspase-3/7 activity. Taken together, our results demonstrated that miR-497 can inhibit growth and induce apoptosis by caspase-3 activation in prostate cancer cells, which suggest its use as a potential therapeutic target in the future.
Keywords
MiR-497; prostate cancer; apoptosis; growth reduction;
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