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http://dx.doi.org/10.7314/APJCP.2013.14.4.2289

Aberrant Expression of miR-20a and miR-203 in Cervical Cancer  

Zhao, Shan (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University)
Yao, De-Sheng (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University)
Chen, Jun-Ying (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University)
Ding, Nan (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.4, 2013 , pp. 2289-2293 More about this Journal
Abstract
MicroRNAs (miRNAs) are small, non-coding RNAs that are critical regulators of various diseases. MicroRNA-20a (miR-20a) and microRNA-203 (miR-203) have previously shown significant alteration in a range of cancers. In this study, the expression levels of miR-20a and miR-203 in 100 cervical cancer tissues were detected by qRT-PCR and compared to patient matched-nontumor cervical tissues. Correlations between expression level and clinicopathologic characteristics of cervical cancer were also analyzed. Finally, we studied the effect of miR-20a and miR-203 on cell proliferation in cervical cancer cell lines by MTT. We found that the expression level of miR-20a (P<0.001) was significantly higher in cervical cancer patients than in healthy controls, while that of miR-203 (P<0.001) was lower. Aberrant expression of miR-20a was correlated with lymph node metastasis (LNM), histological grade and tumor diameter, but down-regulated miR-203 was correlated with LNM only. Furthermore, we found that over-expression of miR-203 decreased cell proliferation, while reduction of miR-20a also prevented tumor progression. Our results support the involvement of miR-20a and miR-203 in cervical tumorigenesis. We propose that miRNAs might be used as therapeutic agents for cervical cancer.
Keywords
miR-20a; miR-203; cervical cancer; therapeutic agents;
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