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http://dx.doi.org/10.7314/APJCP.2013.14.3.1829

In Vitro Cytotoxic Activity of Seed Oil of Fenugreek Against Various Cancer Cell Lines  

Al-Oqail, Mai Mohammad (Department of Pharmacognosy, College of Pharmacy)
Farshori, Nida Nayyar (Department of Pharmacognosy, College of Pharmacy)
Al-Sheddi, Ebtesam Saad (Department of Pharmacognosy, College of Pharmacy)
Musarrat, Javed (Department of Zoology, College of Science, King Saud University)
Al-Khedhairy, Abdulaziz Ali (Department of Zoology, College of Science, King Saud University)
Siddiqui, Maqsood Ahmed (Department of Zoology, College of Science, King Saud University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.3, 2013 , pp. 1829-1832 More about this Journal
Abstract
In the present study, investigations were carried out to screen the anticancer activities of fenugreek seed oil against cancer cell lines (HEp-2, MCF-7, WISH cells), and a normal cell line (Vero cells). Cytotoxicity was assessed with MTT and NRU assays, and cellular morphological alterations were studied using phase contrast light microscopy. All cells were exposed toi 10-1000 ${\mu}g/ml$ of fenugreek seed oil for 24 h. The results show that fenugreek seed oil significantly reduced the cell viability, and altered the cellular morphology in a dose dependent manner. Among the cell lines, HEp-2 cells showed the highest decrease in cell viability, followed by MCF-7, WISH, and Vero cells by MTT and NRU assays. Cell viability at 1000 ${\mu}g/ml$ was recorded as 55% in HEp-2 cells, 67% in MCF-7 cells, 75% in WISH cells, and 86% in Vero cells. The present study provides preliminary screening data for fenugreek seed oil pointing to potent cytotoxicity against cancer cells.
Keywords
Cytotoxicity; cancer cells; fenugreek; cellular morphology;
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1 Amin A, Alkaabi A, Al-Falasi S, et al (2005). Chemopreventive activities of Trigonella foenum graecum (Fenugreek) against breast cancer. Cell Biol Int, 29, 687-94.   DOI   ScienceOn
2 Chen T-J, Jeng J-Y, Lin C-W, et al (2006). Quercetin inhibition of ROS-dependent and independent apoptosis in rat glioma C6 cells. Toxicology, 1-2, 113-126.
3 Danna JL (2006). Medicinal plant specialist group. Vol. 12.Frei E (1982): The national cancer chemotherapy program. Science, 217, 600-06.
4 Das A, Banik NL, Ray SK (2010). Flavonoids activated caspases for apoptosis in human glioblastoma T98G and U87MG cells but not in human normal astrocytes. Cancer, 116, 164-76.
5 Fawzy TKH (1994). Medicinal Plants in Libya. Arab Encyclopedia House. Libya-Fresheny, R.I.A.: Culture of animal cells: A manual of Basic Techniques. 3rd Ed., John Wiley and Sons Inc New York 1985, 486.
6 Gupta SK, Kalaiselvan V, Srivastava S, et al (2010). Trigonella foenum-graecum (Fenugreek) protects against seleniteinduced oxidative stress in experimental cataractogenesis. Biol Trace Elem Res, 136, 258-68.   DOI   ScienceOn
7 Horwitz W (1980). AOCS, Official Methods of Analysis of the Association of Official Analytical Chemist, 13th Edn. Washington DC, USA, 7, 56-132.
8 Johnson IS, Armstrong JG, Gorman M, et al (1963). The vinca alkaloids: A new class of oncolytic agents. Cancer Res, 23, 1390-427.
9 Kaviarasan S, Anuradha CV (2007). Fenugreek (Trigonella foenum graecum) seed polyphenols protect liver from alcohol toxicity: a role on hepatic detoxification system and apoptosis. Pharmazie, 62, 299-304.
10 Kaviarasan S, Nalini R, Palani G, et al (2011). Fenugreek (Trigonella Foenum Graecum) seed extract prevents ethanolinduced toxicity and apoptosis in chang liver cells. Alcohol & Alcoholism, 41, 267-73.
11 Khosla P, Gupta DD, Nagpal RK (1995). Effect of Trigonella foenum graecum (Fenugreek) on serum lipids in normal and diabetic rats. Int J Pharmacol, 27, 89-93.
12 Liu Y, Kakani R, Nair MG (2012). Compounds in functional food fenugreek spice exhibit anti-inflammatory and antioxidant activities. Food Chem, 131, 1187–1192.   DOI   ScienceOn
13 Moon JY, Mosaddik A, Kim H, et al (2011). Cho M, Choi H-K, Kim YS, Cho SK. The chloroform fraction of guava (Psidium cattleianum sabine) leaf extract inhibits human gastric cancer cell proliferation via induction of apoptosis. Food Chem, 125, 369-75.   DOI   ScienceOn
14 Raju J, Bird RP (2006). Alleviation of hepatic steatosis accompanied by modulation of plasma and liver TNF-alpha levels by Trigonella foenum graecum (fenugreek) seeds in Zucker obese (fa/fa) rats. Int J Obes, 30, 1298-307.   DOI
15 Sasipawan M, Weerapreeyakul N, Barusrux S, et al (2011). Cytotoxic and apoptotic effects of six herbal plants against the human hepatocarcinoma (HepG2) cell line. Chin Med, 6, 39.   DOI   ScienceOn
16 Sharma RD, Raghuram TC (1990). Hypoglycaemic effect of fenugreek seeds in non-insulin dependent diabetic subjects. Nutr Res, 10, 731-39.   DOI   ScienceOn
17 Thirunavukkarasu V, Anuradha CV, Viswanathan P (2003). Protective effect of Fenugreek (Trigonella foenum graecum) seeds in experimental ethanol toxicity. Phyto Res,17, 737-43.   DOI   ScienceOn
18 Siddiqui MA, Singh G, Kashyap MP, et al (2008). Influence of cytotoxic doses of 4-hydroxynonenal on selected neurotransmitter receptors in PC-12 cells. Toxicol. In Vitro, 22, 1681-88.   DOI   ScienceOn
19 Siddiqui MA, Kashyap MP, Kumar V, et al (2010). Protective potential of trans-resveratrol against 4-hydroxynonenal induced damage in PC12 cells. Toxicol In Vitro, 24, 1592-98.   DOI   ScienceOn
20 Talib WH, Mahasneh AM (2011). Antiproliferative activity of plant extracts used against cancer in traditional medicine. Sci Pharm, 78, 33-45.
21 Vijayan P, Vijayaraj P, Setty PH C, et al (2004). The cytotoxic activity of the total alkaloids isolated from different parts of Solanum pseudocapsicum. Biol Pharm Bull, 27, 528-30.   DOI   ScienceOn
22 Vijayarathna S, Sreenivasan S (2012). Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines. Asian Pac J Trop Biomed, 2, 826-9.   DOI   ScienceOn
23 Wall MEMC, Wani CE, Cook KH, et al (1966). Palmer ATM, Sim GA. Plant Antitumor Agents. I. The isolation and structure of camptothecin, a novel alkaloidal leukemia and tumor inhibitor from Camptotheca acuminata. J Amer Chem Soc, 88, 3888-90.   DOI
24 Wani C, Taylor HL, Wall ME, et al (1971). Coggon P, McPhail A: Plant antitumor agents VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. J Amer Chem Soc, 93, 2325-27.   DOI
25 World Health Organization (WHO) (1998). World Health Statistics Annual 1996, World Health Organization, Geneva.