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http://dx.doi.org/10.7314/APJCP.2013.14.2.891

Effect of Botulinum Toxin A on Proliferation and Apoptosis in the T47D Breast Cancer Cell Line  

Bandala, Cindy (Department of Research Support, Instituto Nacional de Rehabilitacion)
Perez-Santos, Jose Luis Martin (Vice-rectory of Research and Postgraduate Studies, Benemerita Universidad Autonoma de Puebla)
Lara-Padilla, Eleazar (Molecular Oncology and Oxidative Stress Laboratory, Escuela Superior de Medicina, Instituto Politecnico Nacional)
Delgado Lopez, Ma. Guadalupe (Laboratory of Cellular Biology, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Anaya-Ruiz, Maricruz (Laboratory of Cellular Biology, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.2, 2013 , pp. 891-894 More about this Journal
Abstract
The present study was performed to assess the activity of the botulinum toxin A on breast cancer cells. The T47D cell line was exposed to diverse concentrations of the botulinum toxin A and cell viability and apoptosis were estimated using MTT and propidium iodine/annexin V methods, respectively. Botulinum toxin A exerted greater cytotoxic activity in T47D cells in comparison with MCF10A normal cells; this appeared to be via apoptotic processes caspase-3 and -7. In conclusion, botulinum toxin A induces caspase-3 and -7 dependent apoptotic processes in the T47D breast cancer cell line.
Keywords
Breast cancer; botulinum toxin A; apoptosis; caspases;
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Times Cited By KSCI : 9  (Citation Analysis)
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1 Hassan ZK, Elamin MH, Daghestani MH, et al (2012). Oleuropein induces anti-metastatic effects in breast cancer. Asian Pac J Cancer Prev. 13, 4555-4559.   과학기술학회마을   DOI   ScienceOn
2 Hassan ZK, Daghestani MH (2012). Curcumin effect on MMPs and TIMPs genes in a breast cancer cell line. Asian Pac J Cancer Prev, 13, 3259-64.   과학기술학회마을   DOI   ScienceOn
3 Karsenty G, Rocha J, Chevalier S, et al (2009). Botulinum toxin type A inhibits the growth of LNCaP human prostate cancer cells in vitro and in vivo. Prostate, 69, 1143-50.   DOI   ScienceOn
4 Kim SH, Park IH, Lee H, et al (2012). Efficacy of exemestane after nonsteroidal aromatase inhibitor use in metastatic breast cancer patients. Asian Pac J Cancer Prev, 13, 979-83.   과학기술학회마을   DOI   ScienceOn
5 Kominami K, Nakabayashi J, Nagai T, et al (2012). The molecular mechanism of apoptosis upon caspase-8 activation: quantitative experimental validation of a mathematical model. Biochim Biophys Acta, 1823, 1825-40.   DOI   ScienceOn
6 Lee NK, Shin KH, Park IH, Lee KS, Ro J (2012). Stage-to-stage comparison of neoadjuvant chemotherapy versus adjuvant chemotherapy in pathological lymph node positive breast cancer patients. Jpn J Clin Oncol, 42, 995-1001.   DOI   ScienceOn
7 Mazo EB, Krivoborodov GG, Shkol'nikov ME, Efremov NS (2008). Botulinic toxin of type A in the treatment of prostatic adenoma and cancer. Urologia, 4, 63-6.
8 Nam HJ, Kang JK, Chang JS, et al (2012). Cells transformed by PLC-gamma 1 overexpression are highly sensitive to clostridium difficile toxin A-induced apoptosis and mitotic inhibition. J Microbiol Biotechnol, 22, 50-7.   과학기술학회마을   DOI   ScienceOn
9 Proietti S, Nardicchi V, Porena M, Giannantoni A (2012). Botulinum toxin type-A toxin activity on prostate cancer cell lines. Urologia, 79, 135-41.   DOI
10 Saraydin SU, Tuncer E, Tepe B, et al (2012). Antitumoral effects od Melissa officinalis on breast cancer in vitro and in vivo. Asian Pac J Cancer Prev, 13, 2765-70.   과학기술학회마을   DOI   ScienceOn
11 Sendur MA, Aksoy S, Ozdemir NY, Zengin N, Altundag K (2012). What is the mechanism of progression with Trastuzumab treatment-escape or resistance? Asian Pac J Cancer Prev. 13, 5929-30.   과학기술학회마을   DOI   ScienceOn
12 Smith RA, Duffy SW, Tabar L (2012). Breast cancer screening: the evolving evidence. Oncology. 26, 471-5.
13 Tegenge MA, Bohnel H, Gessler F, Bicker G (2012). Beurotransmitter vesicle release from human model neurons (NT2) is sensistive to botulinum toxin A. Cell Mol Neurobiol, 32, 1021-9.   DOI   ScienceOn
14 Thirunavukkarasusx N, Ghosal KJ, Kukreja R, et al (2011). Microarray analysis of differentially regulated genes in human neuronal and epithelial cell lines upon exposure to type A botulinum neurotoxin. Biochem Biophys Res Commun, 405, 684-90.   DOI   ScienceOn
15 Truin W, Voogd AC, Vreugdenhil G (2012). Effect of adjuvant chemotherapy in postmenopausal patients with invasive ductal versus lobular breast cancer. Ann Oncol. 23, 2859-65.   DOI   ScienceOn
16 Yang L, Sun TT, Wang N (2012). The incidence and mortality trends of female breast cancer in Beijing, China: between 2004 and 2008. Zhonghua Yu Fang Yi Xue Za Zhi, 46, 1009-14.
17 Yurinskaya VE, Moshkov AV, Wibberley AV (2012) Dual response of human leukemia U937 cells to hypertonic shrinkage: initial regulatory volume increase (RVI) and delayed apoptotic volume decrease (AVD). Cell Physiol Biochem, 30, 964-73.   DOI   ScienceOn
18 Edge SB (2013). Advances in breast surgery, 2002-2012. J Natl Compr Canc Netw. 11, 53-9.
19 Zhou SF, Shi WF, Meng D (2012). Interoperative radiotherapy of seventy-two cases of early breast cancer patients during breast-conserving surgery. Asian Pac J Cancer Prev, 13, 1131-5.   과학기술학회마을   DOI   ScienceOn
20 Bray F, Ren JS, Masuyer E, Ferlay J (2013). Global estimates of cancer prevalence for 27 sites in adult population in 2008. Int J Cancer, 132, 1133-45.   DOI   ScienceOn
21 Gorgal T, Charrua A, Silva JF, et al (2012). Expression of apoptosis-regulating genes in the rat prostate following botulinum toxin type A injection. BMC Urol, 10, 12.
22 Haghighat S, Akbari M, Ghaffari S (2012). Standardized breast cancer mortality rate compared to the general female population of Iran. Asian Pac J Cancer Prev, 13, 5525-8.   과학기술학회마을   DOI   ScienceOn
23 Hamedeyazdan S, Fathiazad F, Sharifi S, Nazemiyeh H (2012). Antiproliferative activity of Marrubium persicum extract in the MCF-7 human breast cancer cell line. Asian Pac J Cancer Prev. 13, 5843-8.   과학기술학회마을   DOI   ScienceOn
24 Hasegawa Y, Shimizu T, Takahashi N, Okada Y (2012). The apoptotic volume decrease is an upstream event of MAP kinase activation during staurosporine-induced apoptosis in HeLa cells. Int J Mol Sci, 13, 9363-79.   DOI