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http://dx.doi.org/10.7314/APJCP.2013.14.2.829

MicroRNA-21 Regulates the Invasion and Metastasis in Cholangiocarcinoma and May Be a Potential Biomarker for Cancer Prognosis  

Huang, Qiang (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
Liu, Lei (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
Liu, Chen-Hai (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
You, Hao (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
Shao, Feng (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
Xie, Fang (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
Lin, Xian-Sheng (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
Hu, San-Yuan (Department of General Surgery, Qilu Hospital of Shandong University)
Zhang, Chuan-Hai (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.2, 2013 , pp. 829-834 More about this Journal
Abstract
Background: MicroRNAs are noncoding RNA molecules that posttranscriptionally regulate gene expression. The aim of this study was to determine the role of microRNA-21 in cholangiocarcinomas and its relationship to cholangiocarcinoma RBE cell capacity for invasion and metastasis. Methods: MicroRNA-21 expression was investigated in 41 cases of cholangiocarcinoma samples by in situ hybridization and real-time PCR. Influence on cholangiocarcinoma cell line invasion and metastasis was analyzed with microRNA-21 transfected cells. In addition, regulation of reversion-inducing-cysteine-rich protein with kazal motifs (RECK) by microRNA-21 was elucidated to identify mechanisms. Results: In situ hybridization and real-time quantitative PCR results for patients with lymph node metastasis or perineural invasion showed significantly high expression of microRNA-21 (P<0.05). There was a dramatic decrease in cholangiocarcinoma cell line invasion and metastasis ability after microRNA-21 knockdown (P<0.05). However, overexpression significantly increased invasion and metastasis (P<0.05). Real-time PCR and Western-blot analysis showed that microRNA-21 could potentially inhibit RECK expression in RBE cells. Survival analysis showed that patients with higher expression levels of microRNA-21 more often had a poor prognosis (P<0.05). Conclusions: MicroRNA-21 may play an important role in cholangiocarcinoma invasion and metastasis, suggesting that MicroRNA-21 should be further evaluated as a biomarker for predicting cholangiocarcinoma prognosis.
Keywords
MicroRNA-21; cholangiocarcinoma; invasion; metastasis;
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