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http://dx.doi.org/10.7314/APJCP.2013.14.2.1023

Frequency and Type-distribution of Human Papillomavirus from Paraffin-embedded Blocks of High Grade Cervical Intraepithelial Neoplasia Lesions in Thailand  

Swangvaree, Sukumarn Sanersak (Gynecology Oncology Division, National Cancer Institute)
Kongkaew, Phon (Cytological Unit, Pathological Division, National Cancer Institute)
Ngamkham, Jarunya (Research Division, National Cancer Institute)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.2, 2013 , pp. 1023-1026 More about this Journal
Abstract
Cervical cancer is the most important female gynecological cancer, the second leading cause of cancer mortality in women worldwide and the second most common cancer in Thai women. The major cause of cervical cancer is persistent infection of human papillomavirus (HPV), leading to abnormal epithelial lesions, with progression to precancerous and invasive cancer. This study was conducted to investigate the frequency and type distribution of HPV in Thai women who had abnormal cytology. HPV detection from FFPE confirmed abnormal of high grade cervical intraepithelial lesions were for SPF-10-Innogenic Line Probe Assay. HPV-positivity was detected in 320/355 cases (90.14%) and HPV-negativity in 35/355 (9.86%). HPV-positive was found 147/320 cases (41.4%) of single infection, whereas 173/320 cases (48.7%) showed the multiple HPV infection. The most common seven types were HPV-16, -52, -18, -11, -51, -31 and -33, in that order. HPV 16 and 18, the important oncogenic HPV type, were observed in 64.8% of HSIL cases. Interestingly, a high proportion of multiple infections was found in this study and more than ten types could be detected in one case. Therefore, HPV infection screening program in women is essential, particularly in Thailand. Effective primary and secondary prevention campaigns that reinforce HPV screening for HPV detection and typing may be decrease the incidence and mortality of cervical cancer in the future and may lead to significantly improve the quality of life in Thai women.
Keywords
Human papillomavirus; frequency; type-distribution; cervical intraepithelial neoplasia; Thailand;
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1 Bello BD, Spinillo A, Alberizzi P, et al (2009). Validation of the SPF10 LiPA human papillomavirus typing assay using formalin-fixed paraffin-embedded cervical biopsy samples. J Clin Microbiol, 47, 2175-80.   DOI   ScienceOn
2 Bharti AC, Shukla S, Mahata S, et al (2010). Human papillomavirus and control of cervical cancer in India. Expert Rev Obstet Gynecol, 5, 329-46.   DOI   ScienceOn
3 Bhatla N, Lal N, Bao YP, et al (2008). A meta-analysis of human papillomavirus type-distribution in women form South Asia: Implication for vaccination. Vaccine, 26, 2811-7.   DOI   ScienceOn
4 Ciapponi A, Bardach A, Glujovsky D, et al (2011). Type-specific hpv prevalence in cervical cancer and high-grade lesion in latin america and the caribbean: systematic review and meta-analysis. PLOS one,6, 1-15.
5 Characterization of human papillomavirus type 16 from infected Thai women with different cytological finding. Virus Genes, 39, 30-8.
6 Correnti M, Medina F, Cavazza ME, et al (2011). Human papillomavirus (HPV) type distribution in cervical carcinoma, low-grade, and high-grade squamous intraepithelial lesion in Venezuelan women. Gynecologic Oncol,121, 517-31.
7 Dowhanick JJ, McBride AA, Howley PM (1995). Suppression of cellular proliferation by the papillomavirus E2 protein, J Virol, 69, 7791-9.
8 Gravitt PE, van Doorn LJ, Quint W, et al (2007). Human papillomavirus (HPV) genotyping using paired exfoliated cervicovaginal cells and paraffin-embedded tissues to highlight difficulties in attributing HPV types to specific lesions. J Clin Microbiol, 45, 3245-50.   DOI   ScienceOn
9 Human Papillomavirus Typing Assay Using Formalin-Fixed Paraffin-Embedded Cervical Biopsy Samples. J Clin Microbiol, 47, 2175-80.
10 International Agency for Research on Cancer (1995). IARC Monograph on the Evaluation of Carcinogenic Risks to Human, Vol 64. Human papillomavius. IARC, Lyon.
11 Jacob MV, van den Brule AJ, Snijder PJ, et al (1996). A non-radioactive PCR enzyme-immunoassay enables a rapid identification of HPV16 and 18 in cervical scrapes after GP5+/6+ PCR. J Med Virol , 49, 223-9.   DOI
12 Lurchachaiwong W, Junyangdikul P, Payungporn S, et al (2009). Entire genome.
13 Mateos Lindemann ML, Sanchez Calvo JM, Chacon de Antonio J, et al (2011). Prevalence and distribution of high-risk genotypes of hpv in women with severe cervical lesion in madrid, spain: importance of detecting genotype 16 and other high-risk genotypes. Adv Prev Med, 10,2011.
14 Munoz N (2000). Human papillomavirus and cancer: the epidemiological evidence. J Clin Virol, 19, 1-5.   DOI   ScienceOn
15 Ramet J, van Esso D, Meszner Z (2011). Positive paper- HPV and the primary prevention of cancer; improving vaccine uptake by paediatricians. Eur J Pediatr, 170, 309-21.   DOI   ScienceOn
16 Van Hamont D, van Ham MA, Bakkers JM, et al (2006). Evaluation of the SPF-10-INNO LiPA human papillomavirus (HPV) genotyping test and the roche linear array HPV genotyping test. J Clin Microbiol, 449, 3122-9.
17 Villiers EM, Fanquet C, Broker TR, et al (2004). Classification of papilloma-virus. Virology, 324, 17-27.   DOI   ScienceOn
18 Walboomers JM, Jacobs MV, Manos MM, et al (1999). Human papillomavirus is a necessary cause of invasive cervical cancer world-wide. J Pathol, 189, 12-9.   DOI   ScienceOn
19 World Health Organization (2010). Human paillomavirus Laboratory Manual. 1st ed. HPV Laboratory Network, WHO: Switzerland.
20 Zur Hausen H (2002). Papillomavirus and cancer: from basic studies to clinical application. Nature Rev, 2, 342-50.   DOI   ScienceOn