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http://dx.doi.org/10.7314/APJCP.2013.14.1.557

Gene Expression Profiling of Intrahepatic Cholangiocarcinoma  

Subrungruang, Ittisak (Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University)
Thawornkuno, Charin (Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University)
Chawalitchewinkoon-Petmitr, Porntip (Department of Protozoology, Faculty of Tropical Medicine, Mahidol University)
Pairojkul, Chawalit (Department of Pathology, Faculty of Medicine, Khon Kaen University)
Wongkham, Sopit (Department of Biochemistry, Faculty of Medicine, Khon Kaen University)
Petmitr, Songsak (Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.1, 2013 , pp. 557-563 More about this Journal
Abstract
Intrahepatic cholangiocarcinoma (ICC) is ranked as one of the top five causes of cancer-related deaths. ICC in Thai patients is associated with infection with the liver fluke, Opisthorchis viverrini, but the molecular basis for development remains unclear. The present study employed a microarray approach to compare gene expression profiles of ICCs and normal liver tissues from the same patients residing in Northeast Thailand, a region with a high prevalence of liver fluke infection. In ICC samples, 2,821 and 1,361 genes were found to be significantly up- and down-regulated respectively (unpaired t-test, p<0.05; fold-change ${\gep}2.0$). For validation of the microarray results, 7 up-regulated genes (FXYD3, GPRC5A, CEACAM5, MUC13, EPCAM, TMC5, and EHF) and 3 down-regulated genes (CPS1, TAT, and ITIH1) were selected for confirmation using quantitative RT-PCR, resulting in 100% agreement. The metallothionine heavy metal pathway contains the highest percentage of genes with statistically significant changes in expression. This study provides exon-level expression profiles in ICC that should be fruitful in identifying novel genetic markers for classifying and possibly early diagnosis of this highly fatal type of cholangiocarcinoma.
Keywords
Intrahepatic cholangiocarcinoma; gene expression profile; metallothione heavy metal pathway;
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