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http://dx.doi.org/10.7314/APJCP.2013.14.1.507

Construction and Expression of an Eukaryotic Expression Vector Containing the IER3 Gene  

Wang, Zhen (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University)
Yu, Hong-Sheng (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University)
Yao, Ru-Yong (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University)
Qiu, Wen-Sheng (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University)
Yue, Lu (Department of Oncology, Affiliated Hospital of Medical College, Qingdao University)
Sui, Ai-Hua (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University)
Liu, Xiang-Ping (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University)
Liu, Shi-Hai (Central Laboratory, Affiliated Hospital of Medical College, Qingdao University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.1, 2013 , pp. 507-510 More about this Journal
Abstract
Background: More and more research indicate that the immediately early response gene 3 (IER3) is involved inmany biological provesses, such as apoptosis and immunoreaction, as well as viral infection, tumorigenesis and tumour progression. Methods: Here we describe the construction of an eukaryotic expression vector containing IER3 gene and its expression in A549 cells as assessed through fluorescence microscopyand Western-blotting. Results: Fluorescence detection displayed that GFP in cytoplasm was high during 48 and 72 hours post-transfection. In addition, Western blotting showed significant increase in IER3 gene expression in the transfected cells compared with controls. Conclusion: The recombinate plasmid expression vector was constructed successfully, which may provide a basis for further exploration of function of IER3 in lung cancer.
Keywords
IER3 gene; eukaryotic expression vector; tumor;
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