Browse > Article
http://dx.doi.org/10.7314/APJCP.2013.14.1.195

Clinicopathological Significance of Reduced SPARCL1 Expression in Human Breast Cancer  

Cao, Fang (Department of Surgery, the Affiliated Kunshan First People's Hospital, Jiangsu University)
Wang, Kuo (Department of Surgery, the Affiliated Kunshan First People's Hospital, Jiangsu University)
Zhu, Rong (Department of Surgery, the Affiliated Kunshan First People's Hospital, Jiangsu University)
Hu, Yong-Wei (Department of Surgery, the Affiliated Kunshan First People's Hospital, Jiangsu University)
Fang, Wen-Zheng (Department of Oncology Fuzhou General Hospital of Nanjing Command)
Ding, Hou-Zhong (Department of Surgery, the Affiliated Kunshan First People's Hospital, Jiangsu University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.1, 2013 , pp. 195-200 More about this Journal
Abstract
Secreted protein acidic and rich in cysteines-like protein 1 (SPARCL1), an extracellular matrix glycoprotein, has been implicated in the pathogenesis of several disorders including cancer. However, little is known about the expression and significance of SPARCL1 in human breast cancer. The aim of this study was to determine the expression pattern and clinicopathological significance of SPARCL1 in a Chinese breast cancer cohort. mRNA and protein expression of SPARCL1 in human breast cancer cell lines and breast cancer tissues was detected using the reverse transcription-polymerase chain reaction, real-time quantitative PCR, and Western blotting, respectively. Immunostaining of SPARCL1 in 282 Chinese breast cancer samples was examined and associations with clinicopathological parameters were analyzed. Compared to the positive expression in immortalized human breast epithelial cells, SPARCL1 was nearly absent in human breast cancer cell lines. Similarly, a significantly reduced expression of SPARCL1 was observed in human breast cancer tissues compared to that in normal breast epithelial tissues, for both mRNA and protein levels (P < 0.001). Immunohistochemical analysis showed that strong cytoplasmic immunostaining of SPARCL1 was observed in almost all normal breast samples (43/45) while moderate and strong immunostaining of SPARCL1 was only detected in 191 of 282 (67.7%) breast cancer cases. Moreover, down-regulation of SPARCL1 was significantly correlated with lymphatic metastasis (P = 0.020) and poor grade (P = 0.044). In conclusion, SPARCL1 may be involved in the breast tumorigenesis and serve as a promising target for therapy of breast cancer.
Keywords
Breast cancer; SPARCL1; RT-PCR; immunohistochemistry;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Rosman DS, Kaklamani V, Pasche B (2007). New insights into breast cancer genetics and impact on patient management. Curr Treat Options Oncol, 8, 61-73.   DOI   ScienceOn
2 Russo J, Calaf G, Sohi N, et al (1993). Critical steps in breast carcinogenesis. Ann N Y Acad Sci, 698, 1-20.   DOI
3 Shen FR, Liu M, Zhang X, et al (2012). Health-related quality of life among breast cancer patients and its Influencing Factor in a Chinese Population. Asian Pac J Cancer Prev, 13, 3747-50.   DOI   ScienceOn
4 St Croix B, Rago C, Velculescu V, et al (2000). Genes expressed in human tumor endothelium. Science, 289, 1197-202.   DOI   ScienceOn
5 Sullivan MM, Sage EH (2004). Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol, 36, 991-6.   DOI   ScienceOn
6 Zhang H, Widegren E, Wang DW, Sun XF (2011). SPARCL1: a potential molecule associated with tumor diagnosis, progression and prognosis of colorectal cancer. Tumour Biol, 32, 1225-31.   DOI   ScienceOn
7 Zaravinos A, Lambrou GI, Boulalas I, Delakas D, Spandidos DA (2011). Identification of common differentially expressed genes in urinary bladder cancer. PLoS One, 6, e18135.   DOI   ScienceOn
8 Isler SG, Schenk S, Bendik I, et al (2001). Genomic organization and chromosomal mapping of SPARC-like 1, a gene down regulated in cancers. Int J Oncol, 18, 521-6.
9 Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90.   DOI
10 Kallioniemi OP, Wagner U, Kononen J, Sauter G (2001). Tissue microarray technology for high-throughput molecular profiling of cancer. Hum Mol Genet, 10, 657-62.   DOI   ScienceOn
11 Kataja V, Castiglione M, ESMO Guidelines Working Group (2008). Locally recurrent or metastatic breast cancer: ESMO clinical recommendations for diagnosis, treatment and follow-up. Ann Oncol, 19, ii11-3.   DOI
12 Kononen J, Bubendorf L, Kallioniemi A, et al (1998). Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med, 4, 844-7.   DOI   ScienceOn
13 Lau CP, Poon RT, Cheung ST, Yu WC, Fan ST (2006). SPARC and Hevin expression correlate with tumour angiogenesis in hepatocellular carcinoma. J Pathol, 210, 459-68.   DOI   ScienceOn
14 Li P, Qian J, Yu G, et al (2012). Down-regulated SPARCL1 is associated with clinical significance in human gastric cancer. J Surg Oncol, 105, 31-7.   DOI   ScienceOn
15 Long N, Moore MA, Chen W, et al (2010). Cancer epidemiology and control in north-East Asia - past, present and future. Asian Pac J Cancer Prev, 11 suppl 2, 107-48.
16 Mencalha AL, Levinsphul A, Deterling LC, Pizzatti L, Abdelhay E (2008). SPARC-like1 mRNA is overexpressed in human uterine leiomyoma. Mol Med Report, 1, 571-4.
17 Nelson PS, Plymate SR, Wang K, et al (1998). Hevin, an antiadhesive extracellular matrix protein, is down-regulated in metastatic prostate adenocarcinoma. Cancer Res, 58, 232-6.
18 Bendik I, Schraml P, Ludwig CU (1998). Characterization of MAST9/Hevin, a SPARC-like protein, that is downregulated in non-small cell lung cancer. Cancer Res, 58, 626-9.
19 Claeskens A, Ongenae N, Neefs JM, et al (2000). Hevin is down-regulated in many cancers and is a negative regulator of cell growth and proliferation. Br J Cancer, 82, 1123-30.   DOI   ScienceOn
20 Eheman C, Henley SJ, Ballard-Barbash R, et al (2012). Annual Report to the Nation on the status of cancer, 1975-2008, featuring cancers associated with excess weight and lack of sufficient physical activity. Cancer, 118, 2338-66.   DOI   ScienceOn
21 Esposito I, Kayed H, Keleg S, et al (2007). Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia, 9, 8-17.   DOI
22 Hu H, Zhang H, Ge W, et al (2012). Secreted protein acidic and rich in cysteines-like 1 suppresses aggressiveness and predicts better survival in colorectal cancers. Clin Cancer Res, 18, 5438-48.   DOI   ScienceOn
23 Girard JP, Springer TA (1995). Cloning from purified high endothelial venule cells of hevin, a close relative of the antiadhesive extracellular matrix protein SPARC. Immunity, 2, 113-23.   DOI   ScienceOn
24 Girard JP, Springer TA (1996). Modulation of endothelial cell adhesion by hevin, an acidic protein associated with high endothelial venules. J Biol Chem, 271, 4511-7.   DOI
25 Hambrock HO, Nitsche DP, Hansen U, et al (2003). SC1/hevin. An extracellular calcium-modulated protein that binds collagen I. J Biol Chem, 278, 11351-8.   DOI   ScienceOn
26 Hulvat MC, Hansen NM, Jeruss JS (2009). Multidisciplinary care for patients with breast cancer. Surg Clin North Am, 89, 133-76, ix.   DOI   ScienceOn
27 Hurley PJ, Marchionni L, Simons BW, et al (2012). Secreted protein, acidic and rich in cysteine-like 1 (SPARCL1) is down regulated in aggressive prostate cancers and is prognostic for poor clinical outcome. Proc Natl Acad Sci USA, 109, 14977-82.   DOI
28 Isler SG, Ludwig CU, Chiquet-Ehrismann R, Schenk S (2004). Evidence for transcriptional repression of SPARC-like 1, a gene downregulated in human lung tumors. Int J Oncol, 25, 1073-9.