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http://dx.doi.org/10.7314/APJCP.2013.14.12.7601

Gambogenic Acid Induction of Apoptosis in a Breast Cancer Cell Line  

Zhou, Jing (Department of Clinical Medicine, Taizhou People's Hospital)
Luo, Yan-Hong (Department of Oncology, Jiangsu Cancer Hospital)
Wang, Ji-Rong (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University)
Lu, Bin-Bin (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University)
Wang, Ke-Ming (Department of Oncology, Second Affiliated Hospital of Nanjing Medical University)
Tian, Ye (Department of Clinical Medicine, Taizhou People's Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.12, 2013 , pp. 7601-7605 More about this Journal
Abstract
Background: Gambogenic acid is a major active compound of gamboge which exudes from the Garcinia hanburyi tree. Gambogenic acid anti-cancer activity in vitro has been reported in several studies, including an A549 nude mouse model. However, the mechanisms of action remain unclear. Methods: We used nude mouse models to detect the effect of gambogenic acid on breast tumors, analyzing expression of apoptosis-related proteins in vivo by Western blotting. Effects on cell proliferation, apoptosis and apoptosis-related proteins in MDA-MB-231 cells were detected by MTT, flow cytometry and Western blotting. Inhibitors of caspase-3,-8,-9 were also used to detect effects on caspase family members. Results: We found that gambogenic acid suppressed breast tumor growth in vivo, in association with increased expression of Fas and cleaved caspase-3,-8,-9 and bax, as well as decrease in the anti-apoptotic protein bcl-2. Gambogenic acid inhibited cell proliferation and induced cell apoptosis in a concentration-dependent manner. Conclusion: Our observations suggested that Gambogenic acid suppressed breast cancer MDA-MB-231 cell growth by mediating apoptosis through death receptor and mitochondrial pathways in vivo and in vitro.
Keywords
Gambogenic acid; apoptosis; breast cancer; MDA-MB-231 cells;
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