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http://dx.doi.org/10.7314/APJCP.2013.14.12.7551

Plasma Post-operative miR-21 Expression in the Prognosis of Gastric Cancers  

Ma, Guo-Jian (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital)
Gu, Rong-Min (Department of General Surgery, Jiangsu Cancer Hospital)
Zhu, Ming (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital)
Wen, Xu (Department of General Surgery, Jiangsu Cancer Hospital)
Li, Jin-Tian (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital)
Zhang, Yuan-Ying (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital)
Zhang, Xiao-Mei (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital)
Chen, Sen-Qing (Department of Genetics and Molecular Biology, Jiangsu Cancer Hospital)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.12, 2013 , pp. 7551-7554 More about this Journal
Abstract
Tumor-associated microRNAs have been detected in serum or plasma, but whether plasma microRNA-21 (miR-21) could be a potential circulating biomarker for gastric cancer (GC) prognosis in Chinese is still uncertain. Real-time quantitative reverse transcription PCR (qRT-PCR) was employed in this study to compare the relative expression of miR-21 between pre-operative and post-operative paired plasmas from 42 patients with primary GCs. The results showed that the expression levels of miR-21 in the post-operative plasmas were significantly reduced by an average of 18.2 times in all patients when compared to the pre-operative plasmas, and by 22.1 times in the subgroup of patients without family history, while only 1.76 times in the subgroup of patients with a family history. With respect of clinicopathological characteristics, the plasma miR-21 expression was highly associated with differentiation degree and lymph node metastasis rate. The results suggested plasma miR-21 could be a novel potential biomarker for GC prognosis and evaluation of surgery outcomes, especially in patients without a family history.
Keywords
microRNA-21; gastric cancer; quantitative PCR; plasma;
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