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http://dx.doi.org/10.7314/APJCP.2013.14.12.7489

Enhancement of Anti-tumor Activity of Newcastle Disease Virus by the Synergistic Effect of Cytosine Deaminase  

Lv, Zheng (College of Life Science of Northeast Agricultural University)
Zhang, Tian-Yuan (College of Life Science of Northeast Agricultural University)
Yin, Jie-Chao (College of Life Science of Northeast Agricultural University)
Wang, Hui (College of Life Science of Northeast Agricultural University)
Sun, Tian (College of Life Science of Northeast Agricultural University)
Chen, Li-Qun (National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Bai, Fu-Liang (College of Life Science of Northeast Agricultural University)
Wu, Wei (National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences)
Ren, Gui-Ping (College of Life Science of Northeast Agricultural University)
Li, De-Shan (College of Life Science of Northeast Agricultural University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.14, no.12, 2013 , pp. 7489-7496 More about this Journal
Abstract
This study was conducted to investigate enhancement of anti-tumor effects of the lentogenic Newcastle disease virus Clone30 strain (NDV rClone30) expressing cytosine deaminase (CD) gene against tumor cells and in murine groin tumor-bearing models. Cytotoxic effects of the rClone30-CD/5-FC on the HepG2 cell line were examined by an MTT method. Anti-tumor activity of rClone30-CD/5-FC was examined in H22 tumor-bearing mice. Compared to the rClone30-CD virus treatment alone, NDV rClone30-CD/5-FC at 0.1 and 1 MOIs exerted significant cytotoxic effects (P<0.05) on HepG2 cells. For treatment of H22 tumor-bearing mice, recombinant NDV was injected together with 5-FC given by either intra-tumor injection or tail vein injection. When 5-FC was administered by intra-tumor injection, survival for the rClone30-CD/5-FC-treated mice was 4/6 for 80 days period vs 1/6, 0/6 and 0/6 for the mice treated with rClone30-CD, 5-FC and saline alone, respectively. When 5-FC was given by tail vein injection, survival for the rClone30-CD/5-FC-treated mice was 3/6 vs 2/6, 0/6 and 0/6 for the mice treated with rClone30-CD, 5-FC or saline alone, respectively. In this study, NDV was used for the first time to deliver the suicide gene for cancer therapy. Incorporation of the CD gene in the lentogenic NDV genome together with 5-FC significantly enhances cell death of HepG2 tumor cells in vitro, decreases tumor volume and increases survival of H22 tumor-bearing mice in vivo.
Keywords
Cytosine deaminase; NDV; HepG2; H22; suicide gene therapy;
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1 Huber BE, Austin EA, Richards CA, et al (1994). Metabolism of 5-fluorocytosine to 5-fluorouracil in human colorectal tumor cells transduced with the cytosine deaminase gene: significant antitumor effects when only a small percentage of tumor cells express cytosine deaminase. Proc Natl Acad Sci USA, 9, 18302-6.
2 Ichikawa T, Tamiya T, Adachi Y, et al (2000). In vivo efficacy and toxicity of 5-fluorocytosine/cytosine deaminase gene therapy for malignant gliomas mediated by adenovirus. Cancer Gene Ther, 7, 74-82.   DOI   ScienceOn
3 Kaliberova LN, Manna DLD, Krendelchtchikova V, et al (2008). Molecular chemotherapy of pancreatic cancer using novel mutant bacterial cytosine deaminase gene. Mol Cancer Ther, 7, 2845-54.   DOI   ScienceOn
4 Karcher J, Dyckhoff G, Beckhove P, et al (2004). Anti-tumor vaccination in patients with head and neck squamous cell carcinomas with autologous virus-modified tumor cells. Cancer Res, 64, 8057-61.   DOI   ScienceOn
5 Khatri A, Zhang B, Doherty E, et al (2006). Combination of cytosine deaminase with uracil phosphoribosyl transferase leads to local and distant bystander effects against RM1 prostate cancer in mice. J Gene Med, 8, 1086-96.   DOI   ScienceOn
6 Knipe DM, Howley PM, Griffin DE, et al (2006). Fields virology. 5th ed, Philly, Lippincott Williams & Wilkins.
7 Liang W, Wang H, Sun TM, et al (2003). Application of autologous tumor cell vaccine and NDV vaccine in treatment of tumors of digestive tract. World J Gastroenterol, 9, 495-98.   DOI
8 Nakaya T, Cros J, Park MS, et al (2001). Recombinant Newcastle disease virus as a vaccine vector. J Virol, 75, 11868-73.   DOI   ScienceOn
9 Negroni L, Samson M, Guigonis J, et al (2007). Treatment of colon cancer cells using the cytosine deaminase/5-fluorocytosine suicide system induces apoptosis, modulation of the proteome and $Hsp90\beta$ phosphorylation. Mol Cancer Ther, 6, 2747-56.   DOI
10 Ockert D, Schirrmacher V, Beck N, et al (1996). Newcastle disease virus-infected intact autologous tumor cell vaccine for adjuvant active specific immunotherapy of resected colorectal carcinoma. Clin Cancer Res, 2, 21-28.
11 Patyar S, Joshi R, Byrav DSP, et al (2010). Bacteria in cancer therapy: a novel experimental strategy. J Biomed Sci, 17, 21-29.   DOI   ScienceOn
12 Peeters BP, Leeuw OS, Koch G, et al (1999). Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. J Virol, 73, 5001-9.
13 Pierrefite-Carle V, Baque P, Gavelli A, et al (1999). Cytosine deaminase/5-fluorocytosine-based vaccination against liver tumors: evidence of distant bystander effect. J Natl Cancer Inst, 91, 2014-19.   DOI
14 Ravindra PV, Tiwari AK, Romer-Oberdorfer A, et al (1999). Generation of recombinant lentogenic Newcastle disease virus from cDNA. J Gen Virol, 80, 2987-95.   DOI
15 Schirrmacher V, Haas C, Bonifer R, et al (1999). Human tumor cell modification by virus infection: an efficient and safe way to produce cancer vaccine with pleiotropic immune stimulatory properties when using Newcastle disease virus. Gene Ther, 6, 63-73.   DOI   ScienceOn
16 Schlag P, Manasterski M, Gerneth T, et al (1992). Active specific immunotherapy with Newcastle-disease-virusmodified autologous tumor cells following resection of liver metastases in colorectal cancer. Cancer Immunol Immunothe, 35, 325-30.   DOI
17 Steiner HH, Bonsanto MM, Beckhove P, et al (2004). Anti-tumor vaccination of patients with glioblastoma multiforme: a pilot study to assess feasibility, safety, and clinical benefit. J Clin Oncol, 22, 4272-81.   DOI   ScienceOn
18 Schneider T, Gerhards R, Kirches E, Firsching R (2001). Preliminary results of active specific immunization with modified tumor cell vaccine in glioblastoma multiforme. J Neurooncol, 53, 39-46.   DOI   ScienceOn
19 Springer CJ (2004). Suicide Gene Therapy, Methods and Reviews, (Totowa) New Jersey.
20 Springer CJ, Niculescu-Duvaz I (1996). Gene-directed enzyme prodrug therapy (GDEPT): choice of prodrugs. Adv Drug Deliv Rev, 22, 351-64.   DOI   ScienceOn
21 Stolworty TS, Aaron MK, Candice LW, et al (2008). Yeast cytosine deaminase mutants with increased thermostability impart sensitivity to 5-fluorocytosine. J Mol Biol, 377, 854-69.   DOI   ScienceOn
22 Topf N, Worgall S, Hackett NR, Crystal RG (1998). Regional 'pro-drug' gene therapy: intravenous administration of an adenoviral vector expressing the E. coli cytosine deaminase gene and systemic administration of 5-fluorocytosine suppresses growth of hepatic metastasis of colon carcinoma. Gene Ther, 5, 507-13.   DOI   ScienceOn
23 Toshiaki T, Duflot-Dance A, Tiraby M, et al (2009). Bystander effect from cytosine deaminase and uracil phosphoribosyl transferase genes in vitro: a partial contribution of gap junctions. Cancer Lett, 282, 43-47.   DOI   ScienceOn
24 Vigil A, Martinez O, Chua MA, Garcia-Sastre A (2008). Recombinant Newcastle disease virus as a vaccine vector for cancer therapy. Mol Ther, 16, 1883-90.   DOI
25 Zhao H, Peeters BP (2003). H. Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication. J Gen Virol, 84, 781-88.   DOI   ScienceOn
26 Wallack MK, Sivanandham M, Balch CM, et al (1998). Surgical adjuvant active specific immunotherapy for patients with stage III melanoma: the final analysis of data from a phase III, randomized, double-blind, multicenter vaccinia melanoma oncolysate trial. J Am Coll Surg, 187, 69-77.   DOI   ScienceOn
27 Zamarin D, Martinez-Sobrido L, Kelly K, et al (2009). Enhancement of oncolytic properties of recombinant Newcastle Disease Virus through antagonism of cellular innate immune responses. Mol Ther, 17, 697-706.   DOI   ScienceOn
28 Zamarin D, Vigil A, Kelly K, et al (2009). Genetically engineered Newcastle disease virus for malignant melanoma therapy. Gene Ther, 16, 796-804.   DOI   ScienceOn
29 Batliwalla, Bateman, Serrano, et al (1998 ). A 15-year followup of AJCC stage III malignant melanoma patients treated postsurgically with Newcastle disease virus (NDV) oncolysate and determination of alterations in the CD8 T cell repertoire. Mol Med, 4, 783-94.
30 Arica B, Calis S, Kas H, S, et al (2002). 5-Fluorouracil encapsulated alginate beads for the treatment of breast cancer. Int J Pharm, 242, 267-69.   DOI   ScienceOn
31 Bentires-Alj M, Hellin AC, Lechanteur C, et al (2000). Cytosine deaminase suicide gene therapy for peritoneal carcinomatosis. Cancer Gene Ther, 7, 20-26.   DOI   ScienceOn
32 Bentires-Alj M, Hellin AC, Lechanteur C, et al (2000). Cytosine deaminase suicide gene therapy for peritoneal carcinomatosis. Cancer Gene Ther, 7, 20-26.   DOI   ScienceOn
33 Cassel WA, Murray DR (1988). Treatment of stage II malignant melanoma patients with a Newcastle disease virus oncolysate. Nat Immun Cell Growth Regul, 7, 351-52.
34 Dachs GU, Hunt MA, Syddall S, et al (2009). Bystander or no bystander for gene directed enzyme prodrug therapy. Molecules, 14, 4517-45.   DOI   ScienceOn
35 Fabian Z, Csatary CM, Szeberenyi J, et al (2007). p53-independent endoplasmic reticulum stress-mediated cytotoxicity of a Newcastle disease virus strain in tumor cell lines. J Virol, 81, 2817-30.   DOI   ScienceOn
36 Frederick MA (2002) (ed). Current Protocols in Molecular Biology, New York, John Wiley & Sons.
37 Fuchita M, Ardiani A, Zhao L, et al (2009). Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo. Cancer Res, 69, 4791-99.   DOI   ScienceOn